Alignments for a candidate for proC in Rhizobium leguminosarum WSM1325

GapMind for Amino acid biosynthesis

Alignments for a candidate for proC in Rhizobium leguminosarum WSM1325

Align Pyrroline-5-carboxylate reductase; P5C reductase; P5CR; PCA reductase; EC 1.5.1.2 (characterized)
to candidate WP_012758436.1 RLEG_RS14900 pyrroline-5-carboxylate reductase

Query= SwissProt::P22008
         (273 letters)



>NCBI__GCF_000023185.1:WP_012758436.1
          Length = 275

 Score =  184 bits (467), Expect = 2e-51
 Identities = 112/272 (41%), Positives = 157/272 (57%), Gaps = 10/272 (3%)

Query: 2   STPRIAFIGAGNMAASLIGGLRAQGVPAAQIRASDPGAE----QRAKIAGEFAIDVVESN 57
           ST  +  IGAGNM  +++ G    GVP + +   DPG           AG   +  + ++
Sbjct: 8   STHPVVLIGAGNMGGAMLSGWLKSGVPGSAVIVVDPGPSPAMLSTISEAGATHLTALPTD 67

Query: 58  AEAVADADVVVLSVKPQAMKAVCQALAPALKPEQLIVSIAAGIPCASLEAWLGQPRPVVR 117
            +A     V+ L+VKPQ M+ V  A+  A+ PE ++VS+AAG     LE  LG+   +VR
Sbjct: 68  LKA----SVLFLAVKPQVMETVLPAVKSAVGPETVVVSVAAGKTLGFLERHLGKAA-MVR 122

Query: 118 CMPNTPALLRQGASGLYANAQVSAAQCEQAGQLLSAVGIALWLDDEAQIDAVTAVSGSGP 177
            MPNTPA++ +G +G +ANA V+  Q E+   LL   G   W+  EA IDAVTA+SGSGP
Sbjct: 123 AMPNTPAMVGRGVTGAFANAGVNDQQRERVHSLLRVSGPVEWVPAEADIDAVTALSGSGP 182

Query: 178 AYFFLLMQAMTDAGEKLGLSRETASRLTLQTALGAAQMALSSEVEPAELRRRVTSPNGTT 237
           AY F L++ M +AG KLGL  + A RL  +T  GA ++   S  + + LR  VTSP GTT
Sbjct: 183 AYVFYLVECMAEAGRKLGLQADLAMRLARETVAGAGELMHQSPDDASRLRENVTSPGGTT 242

Query: 238 EAAIKSFQA-NGFEALVEQALNAASQRSAELA 268
            AA+    A +G + L + AL AA +R+ ELA
Sbjct: 243 AAALAVLMAEDGMQPLFDAALAAARKRAEELA 274


Lambda     K      H
   0.315    0.127    0.348 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 194
Number of extensions: 10
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 273
Length of database: 275
Length adjustment: 25
Effective length of query: 248
Effective length of database: 250
Effective search space:    62000
Effective search space used:    62000
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 47 (22.7 bits)

Align candidate WP_012758436.1 RLEG_RS14900 (pyrroline-5-carboxylate reductase)
to HMM TIGR00112 (proC: pyrroline-5-carboxylate reductase (EC 1.5.1.2))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00112.hmm
# target sequence database:        /tmp/gapView.6145.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00112  [M=263]
Accession:   TIGR00112
Description: proC: pyrroline-5-carboxylate reductase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
    8.2e-82  260.7   3.2    9.2e-82  260.6   3.2    1.0  1  lcl|NCBI__GCF_000023185.1:WP_012758436.1  RLEG_RS14900 pyrroline-5-carboxy


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000023185.1:WP_012758436.1  RLEG_RS14900 pyrroline-5-carboxylate reductase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  260.6   3.2   9.2e-82   9.2e-82       1     263 []      12     273 ..      12     273 .. 0.95

  Alignments for each domain:
  == domain 1  score: 260.6 bits;  conditional E-value: 9.2e-82
                                 TIGR00112   1 iaiiGaGnmgeallsgllkkgakakkeilviers.eeklaalakelgvevtsdaeeavkeadvvllavK 68 
                                               +++iGaGnmg a+lsg+lk+g++  + ++v+++  ++++   + e+g++  ++      +a+v++lavK
  lcl|NCBI__GCF_000023185.1:WP_012758436.1  12 VVLIGAGNMGGAMLSGWLKSGVP-GSAVIVVDPGpSPAMLSTISEAGATHLTALP-TDLKASVLFLAVK 78 
                                               579*****************998.7999999999788888999999988888865.566778******* PP

                                 TIGR00112  69 PqdleevlaelkseektkeklliSilAGvtiekleqlleaekrvvRvmPNtaakvgagvtaiaassevs 137
                                               Pq++e+vl  +ks     e++++S++AG t+  le++l+ ++++vR+mPNt+a+vg+gvt+++a++ v+
  lcl|NCBI__GCF_000023185.1:WP_012758436.1  79 PQVMETVLPAVKS-AVGPETVVVSVAAGKTLGFLERHLG-KAAMVRAMPNTPAMVGRGVTGAFANAGVN 145
                                               *********9999.8889********************8.599************************** PP

                                 TIGR00112 138 eeqkelveellkavGkvveve.eklldavtalsGSgPAfvflliealadagvklGLpreeakelaaqtl 205
                                               ++q+e+v++ll+  G v +v+ e+++davtalsGSgPA+vf+l+e++a+ag klGL++++a++la++t+
  lcl|NCBI__GCF_000023185.1:WP_012758436.1 146 DQQRERVHSLLRVSGPVEWVPaEADIDAVTALSGSGPAYVFYLVECMAEAGRKLGLQADLAMRLARETV 214
                                               ********************************************************************* PP

                                 TIGR00112 206 kGaaklleesgehpalLkdkVtsPgGtTiaglavLeekg.vrsavieaveaavkrseeL 263
                                               +Ga++l+++s +++++L+++VtsPgGtT+a+lavL++++ ++  +++a+ aa kr+eeL
  lcl|NCBI__GCF_000023185.1:WP_012758436.1 215 AGAGELMHQSPDDASRLRENVTSPGGTTAAALAVLMAEDgMQPLFDAALAAARKRAEEL 273
                                               **********************************987655****************998 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (263 nodes)
Target sequences:                          1  (275 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.00u 0.00s 00:00:00.00 Elapsed: 00:00:00.00
# Mc/sec: 10.33
//
[ok]

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis