Align (R)-citramalate synthase (EC 2.3.3.21) (characterized)
to candidate WP_012965904.1 FERP_RS07010 2-isopropylmalate synthase
Query= BRENDA::Q58787 (491 letters) >NCBI__GCF_000025505.1:WP_012965904.1 Length = 489 Score = 504 bits (1297), Expect = e-147 Identities = 258/491 (52%), Positives = 353/491 (71%), Gaps = 3/491 (0%) Query: 1 MMVRIFDTTLRDGEQTPGVSLTPNDKLEIAKKLDELGVDVIEAGSAITSKGEREGIKLIT 60 M V I DTTLRDGEQTPGVSL+ KL IA+ LD LGVD+IEAG+AI S+GE + IK I+ Sbjct: 1 MSVIILDTTLRDGEQTPGVSLSVEQKLMIAEALDNLGVDIIEAGTAIASEGEFKAIKAIS 60 Query: 61 KEGLNAEICSFVRALPVDIDAALECDVDSVHLVVPTSPIHMKYKLR-KTEDEVLETALKA 119 + GL AEICSF R DIDAA + DS+ +V P+S IH+ K KT +++++ +++ Sbjct: 61 EAGLKAEICSFGRIKKEDIDAAADAGADSIFMVAPSSDIHINSKFPGKTREDIIQMSIEM 120 Query: 120 VEYAKEHGLIVELSAEDATRSDVNFLIKLFNEGEKVGADRVCVCDTVGVLTPQKSQELFK 179 VEYAKE GLIVE EDA+R+D F+ KL GE+ GADR+ DTVGVLTP+++Q++ Sbjct: 121 VEYAKERGLIVEFGGEDASRADFEFIKKLLKAGEEAGADRLTFTDTVGVLTPERAQQIMS 180 Query: 180 KITENVNLPVSVHCHNDFGMATANTCSAVLGGAVQCHVTVNGIGERAGNASLEEVVAALK 239 ++ + V PV+ H H+DFG+ATANT AV GGA + H VNG+GERAGNA+LEEVV AL+ Sbjct: 181 ELKKVVKKPVAFHGHDDFGLATANTIFAVKGGADEIHCCVNGLGERAGNAALEEVVMALE 240 Query: 240 ILYGYDTKIKMEKLYEVSRIVSRLMKLPVPPNKAIVGDNAFAHEAGIHVDGLIKNTETYE 299 LYG TKI + +Y S++V +L ++ VPPNK IVG+NAF HE+GIH ++++ +TYE Sbjct: 241 YLYGIKTKINKKAIYPTSKLVEKLTRVVVPPNKPIVGENAFTHESGIHTSAVLRDAKTYE 300 Query: 300 PIKPEMVGNRRRIILGKHSGRKALKYKLDLMGINVSDEQLNKIYERVKEFGDLGKYISDA 359 PI PE++G R I+LGKH+G+ +++ + +G + EQ+ +I +RVKE GD GK ++DA Sbjct: 301 PISPEVIGRSRSIVLGKHAGKASVEAIMKELGYKATKEQMQEILKRVKEIGDKGKRVTDA 360 Query: 360 DLLAIVREVTGKLVEEKIKLDELTVVSGNKITPIASVKLHYKGEDITLIETAYGVGPVDA 419 D+ AI+ V E K++L +L VVSG I P ASVKL G++ +E GVGPVDA Sbjct: 361 DVRAIIETVLQIKRERKVELVDLNVVSGANIMPTASVKLKINGKE--YVEAGVGVGPVDA 418 Query: 420 AINAVRKAISGVADIKLVEYRVEAIGGGTDALIEVVVKLRKGTEIVEVRKSDADIIRASV 479 AINA+++A+ ADI+LV Y V+AI GGTDAL++V+V+L+KG +IV R + DII ASV Sbjct: 419 AINAIKRAVKEYADIELVSYHVDAITGGTDALVDVIVQLKKGDKIVTARGARTDIIMASV 478 Query: 480 DAVMEGINMLL 490 +A +EG+NMLL Sbjct: 479 EAFVEGLNMLL 489 Lambda K H 0.316 0.136 0.373 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 690 Number of extensions: 35 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 491 Length of database: 489 Length adjustment: 34 Effective length of query: 457 Effective length of database: 455 Effective search space: 207935 Effective search space used: 207935 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 52 (24.6 bits)
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory