Align 2-isopropylmalate synthase; Alpha-IPM synthase; Alpha-isopropylmalate synthase; EC 2.3.3.13 (characterized)
to candidate WP_012970588.1 ALVIN_RS06805 2-isopropylmalate synthase
Query= SwissProt::P42455 (616 letters) >NCBI__GCF_000025485.1:WP_012970588.1 Length = 547 Score = 527 bits (1357), Expect = e-154 Identities = 276/564 (48%), Positives = 379/564 (67%), Gaps = 26/564 (4%) Query: 40 RYMPFEVEVEDISLPDRTWPDKKITVAPQWCAVDLRDGNQALIDPMSPERKRRMFELLVQ 99 RY P V ++LP+R WP++ I AP W +VDLRDGNQAL +PM+ E+KRR+++LLV+ Sbjct: 8 RYRPGPV----VNLPNRQWPNRTIREAPIWASVDLRDGNQALHEPMNVEQKRRLWDLLVR 63 Query: 100 MGFKEIEVGFPSASQTDFDFVREIIEKGMIPDDVTIQVLVQAREHLIRRTFEACEGAKNV 159 +GFKEIEVGFP+ASQ D+DFVR +IE+ IP+DVTIQVLVQARE LIRRT+EA G + Sbjct: 64 LGFKEIEVGFPAASQPDYDFVRWLIEEDRIPEDVTIQVLVQAREDLIRRTYEALAGVRQA 123 Query: 160 IVHFYNSTSILQRNVVFRMDKVQVKKLATDAAELIKTIAQDYPDTNWRWQYSPESFTGTE 219 IVH YNSTS +QR+ VF D+ VK +A AE + A+DYP+T W +QYSPES+T TE Sbjct: 124 IVHVYNSTSPVQRDWVFGQDREGVKAIARQGAEWVAQGARDYPETRWTFQYSPESYTATE 183 Query: 220 VEYAKEVVDAVVEVMDPTPENPMIINLPSTVEMITPNVYADSIEWMHRNLNRRDSIILSL 279 +YA EV +AV++V PTPE P IINLP+TVE+ +PNV+AD +E +++RRD+I+LS+ Sbjct: 184 PDYAIEVCEAVLDVWQPTPERPCIINLPATVEVSSPNVFADQVEHFANHISRRDAIVLSV 243 Query: 280 HPHNDRGTGVGAAELGYMAGADRIEGCLFGNGERTGNVCLVTLALNMLTQGVDPQLDFTD 339 H HNDRG V AAEL +AGADR+EG L GNGERTGN L+TLA+N+ +QG+DP+LD + Sbjct: 244 HTHNDRGGAVAAAELALLAGADRVEGTLLGNGERTGNADLLTLAMNLYSQGIDPKLDLSR 303 Query: 340 IRQIRSTVEYCNQLRVPERHPYGGDLVFTAFSGSHQDAVNKGLDAMAAKVQPGASSTEVS 399 +I + C L V RHP+ G+LV+ A+SGSHQDA+ K L Sbjct: 304 PDEIVAVCTECTGLPVHPRHPWVGELVYMAYSGSHQDAIRKCLS---------------- 347 Query: 400 WEQLRDTEWEVPYLPIDPKDVGRDYEAVIRVNSQSGKGGVAYIMKTDHGLQIPRSMQVEF 459 Q D W V YLPIDP D+GR+Y+AVIRVNSQSGKGGVA++++ + GL +PR +QVE Sbjct: 348 -RQTPDAPWNVAYLPIDPADLGRNYQAVIRVNSQSGKGGVAFVLEQEFGLSLPRWLQVEL 406 Query: 460 STVVQNVTDAEGGEVNSKAMWDIFATEYLERTAPVEQIALRVENAQTENEDASITAELIH 519 + +VQ ++ GG +++ + IF ++ AP + R++ N I A + Sbjct: 407 AALVQRESETRGGVLDAAQIRAIFERHFIVDQAPHRLLGYRLD----RNGHDVIEARIGT 462 Query: 520 NGKDVTVDGRGNGPLAAYANALEK-LGIDVEIQEYNQHARTSGDDAEAAAYVLAEVNGRK 578 + + G+G G +AA+ +A + G + + +Y +HA G DAEAAAYV E++G+ Sbjct: 463 PDGERMLHGQGQGAMAAFVDAWTRWSGRSLSVLDYQEHAIGEGTDAEAAAYVRVEIDGQA 522 Query: 579 VWGVGIAGSITYASLKAVTSAVNR 602 + G I ASL+A+ SA+NR Sbjct: 523 ITGAAIDRDTVAASLRALLSALNR 546 Lambda K H 0.316 0.133 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 971 Number of extensions: 34 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 616 Length of database: 547 Length adjustment: 36 Effective length of query: 580 Effective length of database: 511 Effective search space: 296380 Effective search space used: 296380 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 53 (25.0 bits)
Align candidate WP_012970588.1 ALVIN_RS06805 (2-isopropylmalate synthase)
to HMM TIGR00970 (leuA: 2-isopropylmalate synthase (EC 2.3.3.13))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00970.hmm # target sequence database: /tmp/gapView.15818.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00970 [M=564] Accession: TIGR00970 Description: leuA_yeast: 2-isopropylmalate synthase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 4.8e-216 704.5 0.0 5.5e-216 704.4 0.0 1.0 1 lcl|NCBI__GCF_000025485.1:WP_012970588.1 ALVIN_RS06805 2-isopropylmalate Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_000025485.1:WP_012970588.1 ALVIN_RS06805 2-isopropylmalate synthase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 704.4 0.0 5.5e-216 5.5e-216 3 561 .. 7 546 .. 5 547 .] 0.97 Alignments for each domain: == domain 1 score: 704.4 bits; conditional E-value: 5.5e-216 TIGR00970 3 kkykpfkaiklsnrkwpdkvitraprwlsvdlrdGnqalidpmsverkkryfkllvriGfkeievgfps 71 ++y+p ++l+nr+wp++ i ap w svdlrdGnqal +pm+ve+k+r+++llvr+Gfkeievgfp+ lcl|NCBI__GCF_000025485.1:WP_012970588.1 7 RRYRPGPVVNLPNRQWPNRTIREAPIWASVDLRDGNQALHEPMNVEQKRRLWDLLVRLGFKEIEVGFPA 75 69******************************************************************* PP TIGR00970 72 asqtdfdfvreiieqglipddvtiqvltqsreelikrtvealsGakkaivhlynatsdlfrevvfrasr 140 asq d+dfvr +ie+ ip+dvtiqvl+q+re+li+rt eal+G ++aivh+yn+ts+++r+ vf ++r lcl|NCBI__GCF_000025485.1:WP_012970588.1 76 ASQPDYDFVRWLIEEDRIPEDVTIQVLVQAREDLIRRTYEALAGVRQAIVHVYNSTSPVQRDWVFGQDR 144 ********************************************************************* PP TIGR00970 141 eevlalavegsklvrklvkdaaksketrwsfeyspesfsdtelefavevceavkeviepteerpiifnl 209 e v a+a +g + +++ a +++etrw+f+yspes++ te+++a+evceav +v++pt+erp i nl lcl|NCBI__GCF_000025485.1:WP_012970588.1 145 EGVKAIARQG---AEWVAQGARDYPETRWTFQYSPESYTATEPDYAIEVCEAVLDVWQPTPERPCIINL 210 **********...6667788999********************************************** PP TIGR00970 210 patvevatpnvyadsieylstniaerekvilslhphndrGtavaaaelGllaGadrieGclfGnGertG 278 patvev++pnv+ad++e+++++i+ r+ ++ls+h+hndrG avaaael llaGadr+eG l+GnGertG lcl|NCBI__GCF_000025485.1:WP_012970588.1 211 PATVEVSSPNVFADQVEHFANHISRRDAIVLSVHTHNDRGGAVAAAELALLAGADRVEGTLLGNGERTG 279 ********************************************************************* PP TIGR00970 279 nvdlvtlalnlytqGvspnldfsdldeilrvvercnkipvherhpygGdlvvtafsGshqdaikkGlda 347 n dl+tla+nly+qG++p+ld+s dei+ v +c +pvh+rhp+ G+lv+ a+sGshqdai+k l lcl|NCBI__GCF_000025485.1:WP_012970588.1 280 NADLLTLAMNLYSQGIDPKLDLSRPDEIVAVCTECTGLPVHPRHPWVGELVYMAYSGSHQDAIRKCLSR 348 ******************************************************************975 PP TIGR00970 348 ldkkkaaadtlwkvpylpldpkdvgreyeavirvnsqsGkGGvayvlktdlGldlprrlqiefssvvkd 416 + + d+ w+v ylp+dp d+gr+y+avirvnsqsGkGGva+vl ++ Gl+lpr lq+e++ +v+ lcl|NCBI__GCF_000025485.1:WP_012970588.1 349 Q-----TPDAPWNVAYLPIDPADLGRNYQAVIRVNSQSGKGGVAFVLEQEFGLSLPRWLQVELAALVQR 412 4.....45789********************************************************** PP TIGR00970 417 iadskGkelsskeisdlfkeeyllnveqlerislvdyaveddGteskvitavvkikgekkdieGsGnGp 485 ++++G+ l++ +i +f+ ++ + ++ ++ +l y ++ +G + vi+a + + ++ ++G G+G lcl|NCBI__GCF_000025485.1:WP_012970588.1 413 ESETRGGVLDAAQIRAIFERHFIV-DQAPH--RLLGYRLDRNG--HDVIEARIGTPDGERMLHGQGQGA 476 *******************99999.55555..56789******..89********************** PP TIGR00970 486 lsalvdaladllnvdvavadysehalgsGddakaasyvelsvrrasdaekatvwGvGiaedvtsaslra 554 ++a+vda ++ +++v+dy+eha+g G da+aa+yv++ + ++ + G i+ d +aslra lcl|NCBI__GCF_000025485.1:WP_012970588.1 477 MAAFVDAWTRWSGRSLSVLDYQEHAIGEGTDAEAAAYVRVEIDGQA------ITGAAIDRDTVAASLRA 539 ***************************************9998888......89*************** PP TIGR00970 555 vlsavnr 561 +lsa+nr lcl|NCBI__GCF_000025485.1:WP_012970588.1 540 LLSALNR 546 *****98 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (564 nodes) Target sequences: 1 (547 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.02u 0.01s 00:00:00.03 Elapsed: 00:00:00.03 # Mc/sec: 9.82 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory