Align Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I beta; GSI beta; EC 6.3.1.2 (characterized)
to candidate WP_012974532.1 AZL_RS10140 type I glutamate--ammonia ligase
Query= SwissProt::Q3V5W6 (468 letters) >NCBI__GCF_000010725.1:WP_012974532.1 Length = 469 Score = 606 bits (1563), Expect = e-178 Identities = 295/467 (63%), Positives = 357/467 (76%), Gaps = 2/467 (0%) Query: 1 MSKSVQLIKDHDVKWIDLRFTDTKGTQHHVTMPARDALEDDFFEVGKMFDGSSIAGWKGI 60 +SK LIK+HDVK++DLRFTD +G HH +++D FE G MFDGSSIAGWKGI Sbjct: 4 ISKVFDLIKEHDVKYVDLRFTDPRGKLHHTAQHV-STIDEDVFEDGIMFDGSSIAGWKGI 62 Query: 61 EASDMILLPDDDTAVLDPFTEDATLILVCDIIEPSTMQGYDRDPRAIAHRAEEYLKTTGI 120 E SDMIL D TAV+DPF TL ++CD+ +P T Y R PR IA AE+Y + GI Sbjct: 63 EESDMILNLDPTTAVMDPFAAQPTLNILCDVYDPGTGGAYARCPRGIAKAAEKYTASAGI 122 Query: 121 GDTVFAGPEPEFFIFDEVKFKSDISGSMFKIYSEQGSWMSDQDIEGGNKGHRPGVKGGYF 180 GDTVF GPE EFF+FD+VK+ D++ ++ S++G++ S +D E GN GHRPG KGGYF Sbjct: 123 GDTVFFGPEAEFFVFDDVKYSVDMNKVSYEFASDEGAYASGKDYEDGNLGHRPGTKGGYF 182 Query: 181 PVPPFDHDHEIRTAMCNALEEMGQTVEVHHHEVATAGQNEIGVKFNTLVKKADEVQTLKY 240 PV P D +++R M + L EMG VE HHHEVA A Q+E+G+KF+TLV+ AD +Q KY Sbjct: 183 PVAPVDSCNDLRAEMLSVLAEMGVPVEKHHHEVAAA-QHELGIKFDTLVRTADNMQYYKY 241 Query: 241 VVHNVADAYGRTATFMPKPLYGDNGSGMHVHMSIAKDGKNTFAGEGYAGLSETALYFIGG 300 VVHNVA AYG+TATFMPKP++GDNGSGMH H SI KDG+ FAG YA LSE ALY+IGG Sbjct: 242 VVHNVAHAYGKTATFMPKPVFGDNGSGMHCHQSIWKDGQPLFAGNQYADLSELALYYIGG 301 Query: 301 IIKHGKALNGFTNPATNSYKRLVPGFEAPVMLAYSARNRSASIRIPYVNSPRGRRIEARF 360 IIKH KALN FTNP+TNSYKRLVPG+EAPV+LAYSARNRSAS RIPYV SP+G+R+E RF Sbjct: 302 IIKHAKALNAFTNPSTNSYKRLVPGYEAPVLLAYSARNRSASCRIPYVASPKGKRVEVRF 361 Query: 361 PDPAANPYLAFAALLMAGLDGIQNKIHPGDAADKNLYDLPPEEAKEIPQVCGSLKEALEE 420 PDP+ANPYLAFAA+LMAGLDGIQNKIHPGDA DKNLYDLPPEE ++P VCGSL+EAL Sbjct: 362 PDPSANPYLAFAAMLMAGLDGIQNKIHPGDAMDKNLYDLPPEELAKVPTVCGSLREALNS 421 Query: 421 LDKGRAFLTKGGVFSDDFIDAYIALKSEEEIKVRTFVHPLEYELYYS 467 L + FL KG VF+ D I+ YI L++EE + T HP+EY++YYS Sbjct: 422 LREDNEFLKKGDVFTADMIEGYIDLRTEEMMAFETMPHPIEYKMYYS 468 Lambda K H 0.318 0.137 0.409 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 774 Number of extensions: 34 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 468 Length of database: 469 Length adjustment: 33 Effective length of query: 435 Effective length of database: 436 Effective search space: 189660 Effective search space used: 189660 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 51 (24.3 bits)
Align candidate WP_012974532.1 AZL_RS10140 (type I glutamate--ammonia ligase)
to HMM TIGR00653 (glnA: glutamine synthetase, type I (EC 6.3.1.2))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00653.hmm # target sequence database: /tmp/gapView.20724.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00653 [M=462] Accession: TIGR00653 Description: GlnA: glutamine synthetase, type I Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 1.7e-206 672.3 0.0 1.9e-206 672.1 0.0 1.0 1 lcl|NCBI__GCF_000010725.1:WP_012974532.1 AZL_RS10140 type I glutamate--am Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_000010725.1:WP_012974532.1 AZL_RS10140 type I glutamate--ammonia ligase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 672.1 0.0 1.9e-206 1.9e-206 2 462 .] 6 468 .. 5 468 .. 0.99 Alignments for each domain: == domain 1 score: 672.1 bits; conditional E-value: 1.9e-206 TIGR00653 2 evlkllkeenvkfvdlrfvDikGklkkveipvseleeealeegiaFDgssveGfksieesDlllkpdpe 70 +v++l+ke++vk+vdlrf+D +Gkl++ + vs+++e+++e+gi+FDgss+ G+k+ieesD++l dp lcl|NCBI__GCF_000010725.1:WP_012974532.1 6 KVFDLIKEHDVKYVDLRFTDPRGKLHHTAQHVSTIDEDVFEDGIMFDGSSIAGWKGIEESDMILNLDPT 74 6899***************************************************************** PP TIGR00653 71 tlvivPfraekvlrvicdvyepvtkepyerdpRsiakraeeelk.tklgdevyfGpEaEFflfdkvefk 138 t+v++Pf+a+++l++ cdvy+p t+ y r+pR iak ae++ +++gd+v+fGpEaEFf+fd+v+ + lcl|NCBI__GCF_000010725.1:WP_012974532.1 75 TAVMDPFAAQPTLNILCDVYDPGTGGAYARCPRGIAKAAEKYTAsAGIGDTVFFGPEAEFFVFDDVKYS 143 *******************************************999*********************** PP TIGR00653 139 easnssflevdseegewn..reveegnkgykikkkggYfdvepvDkakdirrelvlaleelglevevsH 205 +n+ +e s eg++ +++e gn g+++ +kggYf+v+pvD+ +d+r e++++l+e+g+ ve++H lcl|NCBI__GCF_000010725.1:WP_012974532.1 144 VDMNKVSYEFASDEGAYAsgKDYEDGNLGHRPGTKGGYFPVAPVDSCNDLRAEMLSVLAEMGVPVEKHH 212 ****************99899************************************************ PP TIGR00653 206 HEvataqaEidikfdklvkaaDeivlyKyvvknvakkhGktatFmpKplfgdngsGmHvhlslwkdgen 274 HEva aq+E++ikfd+lv++aD+++ yKyvv+nva+ +GktatFmpKp+fgdngsGmH+h+s+wkdg++ lcl|NCBI__GCF_000010725.1:WP_012974532.1 213 HEVAAAQHELGIKFDTLVRTADNMQYYKYVVHNVAHAYGKTATFMPKPVFGDNGSGMHCHQSIWKDGQP 281 ********************************************************************* PP TIGR00653 275 lfageegyagLsetalyyigGilkHakalaAltnptvnsYkRLvpGyEAPvylaysaknRsaliRiPas 343 lfag++ ya+Lse alyyigGi+kHakal+A+tnp++nsYkRLvpGyEAPv laysa+nRsa+ RiP++ lcl|NCBI__GCF_000010725.1:WP_012974532.1 282 LFAGNQ-YADLSELALYYIGGIIKHAKALNAFTNPSTNSYKRLVPGYEAPVLLAYSARNRSASCRIPYV 349 ******.************************************************************** PP TIGR00653 344 anpkakRiEvRspDpsanpYLafaallmAgldGiknkidpgepldknlyelseeelkelGieqlpesLe 412 a+pk+kR+EvR+pDpsanpYLafaa+lmAgldGi+nki+pg+++dknly+l++eel++ ++++ sL+ lcl|NCBI__GCF_000010725.1:WP_012974532.1 350 ASPKGKRVEVRFPDPSANPYLAFAAMLMAGLDGIQNKIHPGDAMDKNLYDLPPEELAK--VPTVCGSLR 416 **********************************************************..********* PP TIGR00653 413 ealdelesdk..evlkevlgeelieafielkrkEveelrlkvhpvElekyld 462 eal++l +d+ +++++v++ ++ie +i+l+++E+ +++ +hp+E ++y++ lcl|NCBI__GCF_000010725.1:WP_012974532.1 417 EALNSLREDNefLKKGDVFTADMIEGYIDLRTEEMMAFETMPHPIEYKMYYS 468 **********8888899*********************************95 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (462 nodes) Target sequences: 1 (469 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.02u 0.01s 00:00:00.03 Elapsed: 00:00:00.02 # Mc/sec: 8.42 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory