Alignments for a candidate for gltX in Azospirillum lipoferum B510

GapMind for Amino acid biosynthesis

Alignments for a candidate for gltX in Azospirillum lipoferum B510

Align Glutamate--tRNA ligase; Glutamyl-tRNA synthetase; GluRS; EC 6.1.1.17 (characterized)
to candidate WP_012974856.1 AZL_RS12200 glutamate--tRNA ligase

Query= SwissProt::Q8DLI5
         (485 letters)



>NCBI__GCF_000010725.1:WP_012974856.1
          Length = 447

 Score =  254 bits (649), Expect = 4e-72
 Identities = 179/461 (38%), Positives = 233/461 (50%), Gaps = 39/461 (8%)

Query: 1   MTVRVRLAPSPTGNLHIGTARTAVFNWLYARHRGGKFILRIEDTDRERSRPEYTENILEG 60
           M+  VR APSPTG +H+G  R A+ NWL+AR  GG F+ R++DTD ERS  E+ + I   
Sbjct: 1   MSTAVRFAPSPTGRIHVGNVRQALVNWLFARKTGGSFLFRLDDTDEERSTREFADAIAAD 60

Query: 61  LQWLGLTWDEGPYFQSDRLDLYRQAIQTLLDKGLAYYCYCTPEELEALRAEQKAKGQAPR 120
           L WLGLTWD     +SDR   Y +A   L   G  Y CY TPEEL   RA   ++G+ P 
Sbjct: 61  LTWLGLTWDRFAR-ESDRYPRYDEATAALKAAGRLYPCYETPEELNLKRASLVSQGRPPI 119

Query: 121 YDNRHRHLTPEEQAAFEAAGRTPVIRFKIEDDRQIEWQDLVRGRVSWQGADLGGDMVIAR 180
           YD     LT E++A  E  GR    RFK+     +EW DLVRG V ++G  L   ++I  
Sbjct: 120 YDRAALRLTDEDRARLEGEGRKAHWRFKL-TPTPVEWTDLVRGPVRFEGTALSDPVLIR- 177

Query: 181 AAPRGEIGYPLYNLVVVVDDIAMGITDVIRGEDHIGNTPKQILLYEALGATPPNFAHTPL 240
                E G PLY L  VVDD+   IT VIRGEDH+ NT  QI ++EA+GA  P FAH PL
Sbjct: 178 -----EDGRPLYTLTSVVDDVDFAITHVIRGEDHVANTAVQIQIFEAMGAAVPVFAHLPL 232

Query: 241 ILNSTGQKLSKRDGVTSISDFRAMGYLAP-ALANYMTLLGWSPPEGVGELFTLDLAAKHF 299
           + ++ GQ LSKR G  S+   R    + P A+ + +  LG S P  +    TLD  A  F
Sbjct: 233 LTDAGGQGLSKRLGSLSVGSLRDEDGIEPMAVNSLLAKLGTSDP--IEARLTLDELAAEF 290

Query: 300 SFERINKAGARFDWDKLNWLNRQYIQQLEPEEFLAELIPL--WQGAGYAFDEERDRPWLF 357
              ++ +   +FD ++L  LN + I  L P E +A  +      GA  AF       W  
Sbjct: 291 DLSKVARGTPKFDPEELGRLNAR-ILHLTPFEAVASRLEALGLTGADAAF-------W-- 340

Query: 358 DLAQLLQPGLNTLREAIDQGAVFFIPSVTFDSEAMAQLGQPQSATILAYLLEHLPAEPAL 417
              + ++P L  L +A D  AV   P               + A  LA     LP EP  
Sbjct: 341 ---EAVRPNLARLDDARDWWAVTHAPVAPL----------VEDAGFLAQAAALLPDEPWD 387

Query: 418 TVAMGQQLIQQAAKAAGVKKGATM-RTLRAALTGAVHGPDL 457
               G      A K A  +KG  +   LR ALTG  HGP+L
Sbjct: 388 LGTWGAW--TNAVKGATGRKGKELFLPLRRALTGRDHGPEL 426


Lambda     K      H
   0.320    0.136    0.418 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 633
Number of extensions: 39
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 485
Length of database: 447
Length adjustment: 33
Effective length of query: 452
Effective length of database: 414
Effective search space:   187128
Effective search space used:   187128
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis