Protein WP_012975006.1 in Azospirillum sp. B510
Annotation: NCBI__GCF_000010725.1:WP_012975006.1
Length: 896 amino acids
Source: GCF_000010725.1 in NCBI
Candidate for 5 steps in Amino acid biosynthesis
| Pathway | Step | Score | Similar to | Id. | Cov. | Bits | Other hit | Other id. | Other bits |
|---|
| L-isoleucine biosynthesis | leuC | lo | 3-isopropylmalate dehydratase large subunit 2; EC 4.2.1.33; Alpha-IPM isomerase 2; IPMI 2; Isopropylmalate isomerase 2 (uncharacterized) | 30% | 71% | 129.8 | Aconitate hydratase A; ACN; Aconitase; (2R,3S)-2-methylisocitrate dehydratase; (2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydratase; IP210; Iron-responsive protein-like; IRP-like; Major iron-containing protein; MICP; Probable 2-methyl-cis-aconitate hydratase; RNA-binding protein; EC 4.2.1.3; EC 4.2.1.99 | 66% | 1192.2 |
| L-leucine biosynthesis | leuC | lo | 3-isopropylmalate dehydratase large subunit 2; EC 4.2.1.33; Alpha-IPM isomerase 2; IPMI 2; Isopropylmalate isomerase 2 (uncharacterized) | 30% | 71% | 129.8 | Aconitate hydratase A; ACN; Aconitase; (2R,3S)-2-methylisocitrate dehydratase; (2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydratase; IP210; Iron-responsive protein-like; IRP-like; Major iron-containing protein; MICP; Probable 2-methyl-cis-aconitate hydratase; RNA-binding protein; EC 4.2.1.3; EC 4.2.1.99 | 66% | 1192.2 |
| L-lysine biosynthesis | lysU | lo | Homoaconitase small subunit; HACN; Homoaconitate hydratase; EC 4.2.1.36 (characterized) | 31% | 89% | 58.2 | Aconitate hydratase A; ACN; Aconitase; (2R,3S)-2-methylisocitrate dehydratase; (2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydratase; IP210; Iron-responsive protein-like; IRP-like; Major iron-containing protein; MICP; Probable 2-methyl-cis-aconitate hydratase; RNA-binding protein; EC 4.2.1.3; EC 4.2.1.99 | 66% | 1192.2 |
| L-isoleucine biosynthesis | leuD | lo | 3-isopropylmalate dehydratase small subunit 2; EC 4.2.1.33; Alpha-IPM isomerase 2; IPMI 2; Isopropylmalate isomerase 2 (uncharacterized) | 33% | 72% | 53.9 | Aconitate hydratase A; ACN; Aconitase; (2R,3S)-2-methylisocitrate dehydratase; (2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydratase; IP210; Iron-responsive protein-like; IRP-like; Major iron-containing protein; MICP; Probable 2-methyl-cis-aconitate hydratase; RNA-binding protein; EC 4.2.1.3; EC 4.2.1.99 | 66% | 1192.2 |
| L-leucine biosynthesis | leuD | lo | 3-isopropylmalate dehydratase small subunit 2; EC 4.2.1.33; Alpha-IPM isomerase 2; IPMI 2; Isopropylmalate isomerase 2 (uncharacterized) | 33% | 72% | 53.9 | Aconitate hydratase A; ACN; Aconitase; (2R,3S)-2-methylisocitrate dehydratase; (2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydratase; IP210; Iron-responsive protein-like; IRP-like; Major iron-containing protein; MICP; Probable 2-methyl-cis-aconitate hydratase; RNA-binding protein; EC 4.2.1.3; EC 4.2.1.99 | 66% | 1192.2 |
Sequence Analysis Tools
View WP_012975006.1 at NCBI
Find papers: PaperBLAST
Find functional residues: SitesBLAST
Search for conserved domains
Find the best match in UniProt
Compare to protein structures
Predict transmenbrane helices: Phobius
Predict protein localization: PSORTb
Find homologs in fast.genomics
Fitness BLAST: loading...
Sequence
MTTFTGQDSLKTRRSLTAGGKSYDYFSLKAAGEAGLGDLSRLPFSMKVLLENLLRFEDGR
TVSVDDVKAVAQWLVDTRSDREIAYRPARVLMQDFTGVPAVCDLAAMREAMASLGGDPAK
INPLVPVDLVIDHSVMVDYFGGADAFQKNVELEFERNLERYAFLRWGQKAFDNFRVVPPG
TGICHQVNTEYLSQVVWTDNDPSGKPVAYPDTLVGTDSHTTMVNGLSVLGWGVGGIEAEA
AMLGQPISMLIPEVVGFRLTGRLKEGTTATDLVLTVTQMLRRKGVVGKFVEFFGPGLDSM
TLPDRATIGNMAPEYGATCGIFPIDAETIRYLTFTGRDPDRVALVEAYAKAQGMWREPGG
PDPVFTDVLELDMGTVEPSLAGPKRPQDRVALSAVAQGFARDMTEAYKADDPRKAVPVKG
AGYSLEQGAVVIAAITSCTNTSNPAVLVAAGLLAKKAVEKGLTQKPWVKTSLAPGSQVVT
DYLAKAGLQPYLDRIGFNIVGYGCTTCIGNSGPLPDAIAAAVEEGNLVVGAVLSGNRNFE
GRVNPHTRANYLASPPLCVAYALAGNLNIDLATDPLGTGGDGQPVYLKDIWPSSREVQDA
IDASLTAEMFRSRYSDVFKGPEQWQAIATAEGQTYQWQDGSTYVKLPPFFSGLTATPDPV
SDVRGARALAVLGDSITTDHISPAGSIKKTSPAGEYLLSYQVRPQDFNSYGARRGNHEVM
MRGTFANIRIRNELIPGVEGGETRHYPSGERLPIYSAAMRYADEGVPLVVVAGKEYGTGS
SRDWAAKGTRLLGVRAVIAESFERIHRSNLVGMGILPLQFKDGVTRADLRLDGSERFDIA
GIEQDLRPRKDVALTLTRADGTVETHTLLLRIDTLDEVEYYRNGGVLNFVLRTLAK
This GapMind analysis is from Jul 25 2024. The underlying query database was built on Jul 25 2024.
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About GapMind
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using
ublast (a fast alternative to protein BLAST)
against a database of manually-curated proteins (most of which are experimentally characterized) or by using
HMMer with enzyme models (usually from
TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
- ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
- HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.
where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").
Otherwise, a candidate is "medium confidence" if either:
- ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
- ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
- HMMer finds a hit (regardless of coverage or other bits).
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps."
For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways.
For diverse bacteria and archaea that can utilize a carbon source, there is a complete
high-confidence catabolic pathway (including a transporter) just 38% of the time, and
there is a complete medium-confidence pathway 63% of the time.
Gaps may be due to:
- our ignorance of proteins' functions,
- omissions in the gene models,
- frame-shift errors in the genome sequence, or
- the organism lacks the pathway.
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory