Alignments for a candidate for prs in Azospirillum lipoferum B510

GapMind for Amino acid biosynthesis

Alignments for a candidate for prs in Azospirillum lipoferum B510

Align Ribose-phosphate pyrophosphokinase; RPPK; EC 2.7.6.1; 5-phospho-D-ribosyl alpha-1-diphosphate synthase; Phosphoribosyl diphosphate synthase; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase; PRPPase (uncharacterized)
to candidate WP_012978320.1 AZL_RS31030 ribose-phosphate pyrophosphokinase

Query= curated2:Q8TUT6
         (291 letters)



>NCBI__GCF_000010725.1:WP_012978320.1
          Length = 308

 Score =  144 bits (364), Expect = 2e-39
 Identities = 98/288 (34%), Positives = 142/288 (49%), Gaps = 7/288 (2%)

Query: 8   EGVKLGRRLAEELDAELAPVEEDRFPDGEQIVRVPPELDGTVVVVHSMSPPQDENLVKAI 67
           E     RRLA  L       E  RFPDGE +VR+P  ++   +V  S+  P D+ LV+  
Sbjct: 21  ESADAARRLAAALGIPCHIAELHRFPDGESLVRLPEPVE-RAIVYRSLDRPNDK-LVELT 78

Query: 68  ITLDAARENGAEEVIAIVPYMAYSRQDRRFEPGEPVSFRAVARAVSANADALITVDLHEP 127
           +     R  GA ++  + PYMAY RQD  F PGEPVS   V   +    D  + V+ H  
Sbjct: 79  LAASVLRRQGATDLCLVAPYMAYMRQDAIFRPGEPVSQTVVGDWLGRCFDRFVCVEPHLH 138

Query: 128 GTLKYFDV----PAENVSAAEELGKYLAERFEGEDLVVIGPDEGARELAREVASICGVEY 183
            T +  +V    P+  +S A  +  +L       D V++GPDE +  L   VA   G+  
Sbjct: 139 RTHRLDEVFVGRPSVALSGAAPIADHLRAAGVTPDTVIVGPDEESAPLVEAVAGPLGLTG 198

Query: 184 DHLEKKRLSGDEVEIH-PKELDVEGRTVVLVDDMIDTGGTMVEAARALRDQGAGTLYAAC 242
               K+R    +V +  P +  + GR VV+VDD+I +G T+   ARA R  GA  L    
Sbjct: 199 IVGRKERRGDRDVTVALPPDAPIHGRPVVIVDDVISSGETIFSCARAARILGADELRVYG 258

Query: 243 THALLTRNAATRLLASGFEDIIATDTVPNPFEKVSVAPPVAEAVENLS 290
            HAL     A R  A G E  ++ D VP+P   +S++  +A+A+ +LS
Sbjct: 259 VHALFGEAVAGRFAAEGLEPPLSCDGVPHPTNALSLSGLIADAIRSLS 306


Lambda     K      H
   0.314    0.134    0.370 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 211
Number of extensions: 13
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 291
Length of database: 308
Length adjustment: 27
Effective length of query: 264
Effective length of database: 281
Effective search space:    74184
Effective search space used:    74184
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 48 (23.1 bits)

Align candidate WP_012978320.1 AZL_RS31030 (ribose-phosphate pyrophosphokinase)
to HMM TIGR01251 (prs: ribose-phosphate diphosphokinase (EC 2.7.6.1))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR01251.hmm
# target sequence database:        /tmp/gapView.19642.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01251  [M=309]
Accession:   TIGR01251
Description: ribP_PPkin: ribose-phosphate diphosphokinase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
    9.3e-49  151.9   0.0    1.1e-48  151.7   0.0    1.0  1  lcl|NCBI__GCF_000010725.1:WP_012978320.1  AZL_RS31030 ribose-phosphate pyr


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000010725.1:WP_012978320.1  AZL_RS31030 ribose-phosphate pyrophosphokinase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  151.7   0.0   1.1e-48   1.1e-48       8     298 ..      21     305 ..      16     308 .] 0.93

  Alignments for each domain:
  == domain 1  score: 151.7 bits;  conditional E-value: 1.1e-48
                                 TIGR01251   8 sskelaekvaknlglelgdvevkkFadgElyvrieesvrgkdvfiivqstsapvndalmellllidalk 76 
                                               +s + a+++a +lg++   +e ++F+dgE  vr+ e v   +++i+  s     nd+l+el l+++ l+
  lcl|NCBI__GCF_000010725.1:WP_012978320.1  21 ESADAARRLAAALGIPCHIAELHRFPDGESLVRLPEPV---ERAIVYRSLD-RPNDKLVELTLAASVLR 85 
                                               56788999*****************************9...6799966666.69*************** PP

                                 TIGR01251  77 rasaksvtaviPyygYaRqdkkaksrepisaklvaklleeaGadrvltvdlHseqiq...gfFd.vpve 141
                                               r++a  + +v+Py++Y Rqd  ++++ep+s  +v++ l     dr + v+ H + ++   ++F   p  
  lcl|NCBI__GCF_000010725.1:WP_012978320.1  86 RQGATDLCLVAPYMAYMRQDAIFRPGEPVSQTVVGDWLGRC-FDRFVCVEPHLHRTHrldEVFVgRPSV 153
                                               *****************************************.********9987664222788878999 PP

                                 TIGR01251 142 nlsaspklieelkkke.lknlvvvsPDkGaverakkvakklglelaiieKeRdskenevevtnllgdve 209
                                                ls++  ++++l++     ++v+v PD+ ++  +++va  lgl   + +KeR  ++ +    + +  + 
  lcl|NCBI__GCF_000010725.1:WP_012978320.1 154 ALSGAAPIADHLRAAGvTPDTVIVGPDEESAPLVEAVAGPLGLTGIVGRKERRGDRDVTVALPPDAPIH 222
                                               *************988899************************999******988877777899***** PP

                                 TIGR01251 210 gkdvvivDDiisTggTlvkaaelLkekGAkkvivaathgvfsgdAlerlaeagveevivtntilveekk 278
                                               g+ vvivDD+is+g T+   a++++  GA +  v+ +h++f +  + r+a  g+e  ++++ +++    
  lcl|NCBI__GCF_000010725.1:WP_012978320.1 223 GRPVVIVDDVISSGETIFSCARAARILGADELRVYGVHALFGEAVAGRFAAEGLEPPLSCDGVPH---- 287
                                               *****************************************************************.... PP

                                 TIGR01251 279 lpkvseisvapliaeaiari 298
                                                   + +s++ lia+ai++ 
  lcl|NCBI__GCF_000010725.1:WP_012978320.1 288 --PTNALSLSGLIADAIRSL 305
                                               ..999************864 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (309 nodes)
Target sequences:                          1  (308 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.00u 0.00s 00:00:00.00 Elapsed: 00:00:00.00
# Mc/sec: 11.73
//
[ok]

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis