Align Gamma-glutamyl phosphate reductase; GPR; Glutamate-5-semialdehyde dehydrogenase; Glutamyl-gamma-semialdehyde dehydrogenase; GSA dehydrogenase; EC 1.2.1.41 (characterized)
to candidate WP_013011161.1 DACET_RS09495 glutamate-5-semialdehyde dehydrogenase
Query= SwissProt::P07004 (417 letters) >NCBI__GCF_000025725.1:WP_013011161.1 Length = 411 Score = 358 bits (919), Expect = e-103 Identities = 183/407 (44%), Positives = 267/407 (65%), Gaps = 5/407 (1%) Query: 1 MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILNANAQDVADARANGLSE 60 MLE M AK+ASY L +++ K+ L IA++LEA I N N +D+ AR GLS+ Sbjct: 1 MLENMFREAKKASYVLMNVNTDVKDSALSAIAEKLEANRVKIKNENLKDLDYARETGLSD 60 Query: 61 AMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSGLRLERRRVPLGVIGVIYE 120 A++DRL L R+ G+ V+ + + DPVG+V+DG +GL + + +VPLGV+G+I+E Sbjct: 61 ALIDRLTLDDKRIDGMIQAVKDIRSQVDPVGKVVDGYKRPNGLYITKVKVPLGVVGIIFE 120 Query: 121 ARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQDALKSCGLPAGAVQAIDNPD 180 +RPNVT+D A+LCLK+GN ILRGGKE +N ++++A+ GLP V I++ D Sbjct: 121 SRPNVTIDAAALCLKSGNVAILRGGKEAVHSNTYLGRLMKEAVAEAGLPKACVNIIEDTD 180 Query: 181 RALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVITGGIGVCHIYVDESVEIAEALK 240 R LV EML+ ID++IPRGG L + C E S IP++ G+CH+Y+D+ +I A+ Sbjct: 181 RGLVMEMLKAKGSIDIMIPRGGKALIEFCTEHSLIPLVKHDDGICHVYIDKFADIEMAVS 240 Query: 241 VIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQMAESGVTLHADAAALAQLQAGPAK 300 + VNAK QR CNT+ETLLV++ +A++ L L K E V L + A Sbjct: 241 IAVNAKCQRVGVCNTMETLLVHEAVAETVLKRLEKAYGEHNVELRGCEHTAELISCKKA- 299 Query: 301 VVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIREHGTQHSDAILTRDMRNAQRFVNEVD 360 E++ E+L L+VK+VS +D+AI HI ++G+ HS++I+T + N++ F+N VD Sbjct: 300 ----AEEDWSTEYLDYILSVKVVSSIDEAIEHINKYGSMHSESIVTENYTNSEMFLNMVD 355 Query: 361 SSAVYVNASTRFTDGGQFGLGAEVAVSTQKLHARGPMGLEALTTYKW 407 ++AVYVNASTR+TDGG+FG+GAE+ +STQKLH RGPMG + LTT K+ Sbjct: 356 AAAVYVNASTRWTDGGEFGMGAEIGISTQKLHCRGPMGADDLTTTKY 402 Lambda K H 0.317 0.133 0.369 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 422 Number of extensions: 14 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 417 Length of database: 411 Length adjustment: 31 Effective length of query: 386 Effective length of database: 380 Effective search space: 146680 Effective search space used: 146680 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 50 (23.9 bits)
Align candidate WP_013011161.1 DACET_RS09495 (glutamate-5-semialdehyde dehydrogenase)
to HMM TIGR00407 (proA: glutamate-5-semialdehyde dehydrogenase (EC 1.2.1.41))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00407.hmm # target sequence database: /tmp/gapView.11758.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00407 [M=398] Accession: TIGR00407 Description: proA: glutamate-5-semialdehyde dehydrogenase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 3.4e-158 512.6 3.8 3.9e-158 512.4 3.8 1.0 1 lcl|NCBI__GCF_000025725.1:WP_013011161.1 DACET_RS09495 glutamate-5-semial Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_000025725.1:WP_013011161.1 DACET_RS09495 glutamate-5-semialdehyde dehydrogenase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 512.4 3.8 3.9e-158 3.9e-158 1 398 [] 9 401 .. 9 401 .. 0.99 Alignments for each domain: == domain 1 score: 512.4 bits; conditional E-value: 3.9e-158 TIGR00407 1 akeaalklaqlstaeknealskiadeLkaeaelilaanakdiaaakenGladalldrLlLteeklksia 69 ak+a++ l++++t+ k++als+ia++L+a+ i ++n kd+ a+e+Gl+dal+drL+L+++++ +++ lcl|NCBI__GCF_000025725.1:WP_013011161.1 9 AKKASYVLMNVNTDVKDSALSAIAEKLEANRVKIKNENLKDLDYARETGLSDALIDRLTLDDKRIDGMI 77 89******************************************************************* PP TIGR00407 70 ddvkdvieLadPvGkvieareldeGLklervrvPlGvlgviyearPevivdvasLclktGnaviLkGgk 138 ++vkd+ + dPvGkv+++ ++ +GL + +v+vPlGv+g+i+e+rP+v++d+a+Lclk+Gn +iL+Ggk lcl|NCBI__GCF_000025725.1:WP_013011161.1 78 QAVKDIRSQVDPVGKVVDGYKRPNGLYITKVKVPLGVVGIIFESRPNVTIDAAALCLKSGNVAILRGGK 146 ********************************************************************* PP TIGR00407 139 eavrsnkalveviqdaleqtglpveavqliedpdreevkellkldeyvdlliPrGgnelvklikeesti 207 eav+sn l +++++a++++glp+ +v++ied+dr v+e+lk +d++iPrGg++l++ + e+s i lcl|NCBI__GCF_000025725.1:WP_013011161.1 147 EAVHSNTYLGRLMKEAVAEAGLPKACVNIIEDTDRGLVMEMLKAKGSIDIMIPRGGKALIEFCTEHSLI 215 ********************************************************************* PP TIGR00407 208 PvlehadGvChiyldesadlakakkvivdaktqrPstCnaietLLvnkaiaeefleeLekqleekgvel 276 P ++h dG+Ch+y+d+ ad+++a + v+ak qr +Cn++etLLv++a+ae l++Lek+ e++vel lcl|NCBI__GCF_000025725.1:WP_013011161.1 216 PLVKHDDGICHVYIDKFADIEMAVSIAVNAKCQRVGVCNTMETLLVHEAVAETVLKRLEKAYGEHNVEL 284 ********************************************************************* PP TIGR00407 277 radalvlkllelekateaevskedfdkeflsldLsvkivedleeaiehirqygtkhsdailtedkknae 345 r+ + + +l++++ka e ed+ +e+l+ +Lsvk+v++++eaiehi++yg+ hs++i+te+ +n+e lcl|NCBI__GCF_000025725.1:WP_013011161.1 285 RGCEHTAELISCKKAAE-----EDWSTEYLDYILSVKVVSSIDEAIEHINKYGSMHSESIVTENYTNSE 348 ************96655.....89********************************************* PP TIGR00407 346 kfvkevdsaavyvnastrfadGfrfGfGaevgistqklharGPvGLeaLvsyk 398 f++ vd+aavyvnastr++dG++fG+Gae+gistqklh rGP+G + L+++k lcl|NCBI__GCF_000025725.1:WP_013011161.1 349 MFLNMVDAAAVYVNASTRWTDGGEFGMGAEIGISTQKLHCRGPMGADDLTTTK 401 **************************************************997 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (398 nodes) Target sequences: 1 (411 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.02u 0.01s 00:00:00.03 Elapsed: 00:00:00.02 # Mc/sec: 7.97 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory