Align aspartate kinase (EC 2.7.2.4) (characterized)
to candidate WP_013074687.1 BTUS_RS03195 aspartate kinase
Query= BRENDA::P08495 (408 letters) >NCBI__GCF_000092905.1:WP_013074687.1 Length = 415 Score = 418 bits (1074), Expect = e-121 Identities = 226/403 (56%), Positives = 291/403 (72%), Gaps = 1/403 (0%) Query: 1 MGLIVQKFGGTSVGSVEKIQNAANRAIAEKQKGHQVVVVVSAMGKSTDELVSLAKAISDQ 60 M IV KFGG+SV + E++ A R K +GHQV VVVSAMG +TD+L++LAK ++++ Sbjct: 1 MARIVMKFGGSSVATAERMVKVAQRVAKTKAEGHQVAVVVSAMGDTTDDLIALAKQVNER 60 Query: 61 PSKREMDMLLATGEQVTISLLSMALQEKGYDAVSYTGWQAGIRTEAIHGNARITDIDTSV 120 P RE+DMLL+TGEQV+I++L+MALQ G A S TG QAG R E + G ARI ++ Sbjct: 61 PPARELDMLLSTGEQVSIAVLTMALQGLGVPATSLTGGQAGFRVEQVFGKARILEVRPDR 120 Query: 121 LADQLEKGKIVIVAGFQGMTEDCEITTLGRGGSDTTAVALAAALKADKCDIYTDVPGVFT 180 + L G + +VAGFQG+T+D EI TLGRGGSDTTAVALAAALKAD C+IYTDV GV+T Sbjct: 121 VDALLRDGHVAVVAGFQGVTDDGEIATLGRGGSDTTAVALAAALKADVCEIYTDVDGVYT 180 Query: 181 TDPRYVKSARKLEGISYDEMLELANLGAGVLHPRAVEFAKNYQVPLEVRSSTETEAGTLI 240 TDPR VK A K+ ISYDEMLELANLGA VLHPRAVE+AK Y+VPL VRSS GT + Sbjct: 181 TDPRIVKEAAKIPEISYDEMLELANLGAVVLHPRAVEYAKLYRVPLVVRSSFHDGPGTWV 240 Query: 241 EEESSMEQNLIVRGIAFEDQITRVTIYGLTSGLTTLSTIFTTLAKRNINVDIIIQTQA-E 299 +EE+++EQ +VRGIA + + +V++ G+ + +L +F LA+ N+NVDII+Q+ Sbjct: 241 KEEANVEQGQVVRGIAHDLNVAKVSLVGVPNRQDSLGKVFHALAEENVNVDIIVQSIVHN 300 Query: 300 DKTGISFSVKTEDADQTVAVLEEYKDALEFEKIETESKLAKVSIVGSGMVSNPGVAAEMF 359 D ISF+V +D T+ VLE+ + AL +I TE LAKVSIVG+GM+SNPGVAA MF Sbjct: 301 DVHDISFTVCRDDLPVTLRVLEDLRVALGAGEIVTEEDLAKVSIVGAGMISNPGVAARMF 360 Query: 360 AVLAQKNILIKMVSTSEIKVSTVVSENDMVKAVESLHDAFELS 402 L + + I+MVSTSEIKVS VV D+ +AV+ LH AFELS Sbjct: 361 DALIEAGMSIRMVSTSEIKVSCVVDARDVKRAVQVLHQAFELS 403 Lambda K H 0.313 0.128 0.337 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 357 Number of extensions: 13 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 408 Length of database: 415 Length adjustment: 31 Effective length of query: 377 Effective length of database: 384 Effective search space: 144768 Effective search space used: 144768 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 50 (23.9 bits)
Align candidate WP_013074687.1 BTUS_RS03195 (aspartate kinase)
to HMM TIGR00656 (aspartate kinase, monofunctional class (EC 2.7.2.4))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00656.hmm # target sequence database: /tmp/gapView.27842.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00656 [M=407] Accession: TIGR00656 Description: asp_kin_monofn: aspartate kinase, monofunctional class Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 1.8e-138 447.9 11.6 2e-138 447.7 11.6 1.0 1 lcl|NCBI__GCF_000092905.1:WP_013074687.1 BTUS_RS03195 aspartate kinase Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_000092905.1:WP_013074687.1 BTUS_RS03195 aspartate kinase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 447.7 11.6 2e-138 2e-138 3 406 .. 3 402 .. 1 403 [. 0.98 Alignments for each domain: == domain 1 score: 447.7 bits; conditional E-value: 2e-138 TIGR00656 3 liVqKFGGtsvgsserikkaakivlkelkegkkvvVVvSAmskvtdelvelaellklleaisdeispre 71 iV+KFGG+sv+ er+ k+a++v k++ eg++v VVvSAm+ +td l++la +++++ + re lcl|NCBI__GCF_000092905.1:WP_013074687.1 3 RIVMKFGGSSVATAERMVKVAQRVAKTKAEGHQVAVVVSAMGDTTDDLIALA------KQVNERPPARE 65 59**************************************************......9********** PP TIGR00656 72 rdelvsvGEllssallssalrelgvkaealdgkeagilTddefgnAkikelateerLlelLeegiivvv 140 d+l+s+GE++s+a+l++al+ lgv a++l+g++ag++ ++ fg+A+i e+ + r+ lL +g + vv lcl|NCBI__GCF_000092905.1:WP_013074687.1 66 LDMLLSTGEQVSIAVLTMALQGLGVPATSLTGGQAGFRVEQVFGKARILEVRP-DRVDALLRDGHVAVV 133 *****************************************************.*************** PP TIGR00656 141 aGFiGateeGeiTtLGRGGSDltAallaaalkAdrveiyTDVeGvyttDPrvveeakkidkisyeEale 209 aGF+G t +Gei tLGRGGSD+tA++laaalkAd +eiyTDV+GvyttDPr+v+ea ki++isy+E+le lcl|NCBI__GCF_000092905.1:WP_013074687.1 134 AGFQGVTDDGEIATLGRGGSDTTAVALAAALKADVCEIYTDVDGVYTTDPRIVKEAAKIPEISYDEMLE 202 ********************************************************************* PP TIGR00656 210 lAtlGakvlhpralelaveakvpilvrsskekeegTlitn..kkensslvkaialeknvarltvegegm 276 lA+lGa vlhpra+e+a+ + vp++vrss++ gT + + + e+ ++v++ia++ nva+++++ g+ lcl|NCBI__GCF_000092905.1:WP_013074687.1 203 LANLGAVVLHPRAVEYAKLYRVPLVVRSSFHDGPGTWVKEeaNVEQGQVVRGIAHDLNVAKVSLV--GV 269 ***************************************965555669*****************..9* PP TIGR00656 277 lgkrgilaeifkaLaeeeinvdlisqtese...tsislvvdeedvdeakkaLkeesgaaelesleveed 342 +++ l ++f aLaee++nvd+i+q+ + +is++v + d++ + ++L++ a++ ++ +eed lcl|NCBI__GCF_000092905.1:WP_013074687.1 270 PNRQDSLGKVFHALAEENVNVDIIVQSIVHndvHDISFTVCRDDLPVTLRVLEDLRVALGAGEIVTEED 338 ***************************988777899********************************* PP TIGR00656 343 lavvsivgaglveapGvaseifkaleekninilmisssetkisvlvdekdaekavrklheklee 406 la+vsivgag++++pGva+++f al e ++ i m+s+se+k+s +vd +d+++av+ lh+++e+ lcl|NCBI__GCF_000092905.1:WP_013074687.1 339 LAKVSIVGAGMISNPGVAARMFDALIEAGMSIRMVSTSEIKVSCVVDARDVKRAVQVLHQAFEL 402 **************************************************************97 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (407 nodes) Target sequences: 1 (415 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01 # Mc/sec: 10.59 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory