Alignments for a candidate for aspS2 in Kyrpidia tusciae DSM 2912

GapMind for Amino acid biosynthesis

Alignments for a candidate for aspS2 in Kyrpidia tusciae DSM 2912

Align aspartate-tRNAAsn ligase (EC 6.1.1.23) (characterized)
to candidate WP_013075119.1 BTUS_RS05485 aspartate--tRNA ligase

Query= BRENDA::O32038
         (592 letters)



>NCBI__GCF_000092905.1:WP_013075119.1
          Length = 598

 Score =  704 bits (1817), Expect = 0.0
 Identities = 352/581 (60%), Positives = 438/581 (75%), Gaps = 2/581 (0%)

Query: 4   RTYYCGDITEKAIGESVTLKGWVQKRRDLGGLIFIDLRDRTGIVQVVFNPDVSKEALAIA 63
           RT  CG +TE  IG  V L GWVQ+RRDLGG+IF+DLRDR+GIVQVV + +  + A A+A
Sbjct: 11  RTAMCGRLTESDIGREVVLSGWVQRRRDLGGVIFLDLRDRSGIVQVVSSQEFGEAAFAVA 70

Query: 64  EGIRNEYVLDIQGKVVAREEGTVNPNLKTGAIEIHADGVNVLNAAKTPPFAISDQAEEVS 123
           + +RNEYV+ ++G VVAR+  TVNPNL+TG IE+ A  V +LN +K  PF I D  + V 
Sbjct: 71  DLVRNEYVISVRGDVVARDPETVNPNLETGTIEVRAREVELLNRSKPTPFPIEDGID-VD 129

Query: 124 EDVRLKHRYLDLRRPAMFQTMQLRHNVTKAVRSFLDENGFLDIETPILTGSTPEGARDYL 183
           E VRL+ RYLDLRRP M +T  LRH   + VR FLD +GF++IETP+LT  TPEGARDYL
Sbjct: 130 ESVRLRFRYLDLRRPEMQRTFLLRHRAMQQVRRFLDLHGFMEIETPMLTRRTPEGARDYL 189

Query: 184 VPSRVHEGEFYALPQSPQLFKQLLMVSGIERYYQIARCFRDEDLRADRQPEFTQIDIEMS 243
           VPSRVH G+FYALPQSPQLFKQLLMVSG ERY+QI RCFRDEDLRADRQPEFTQIDIEMS
Sbjct: 190 VPSRVHPGQFYALPQSPQLFKQLLMVSGFERYFQIVRCFRDEDLRADRQPEFTQIDIEMS 249

Query: 244 FMSQEDIMSLAEEMMAKVMRETKGEELQLPLPRMTYDEAMNKYGSDKPDTRFDMLLTDVS 303
           FM   +   L E MMA++ +E  G  + LP  R+ YDEAM +YGSDKPD RF + + DV+
Sbjct: 250 FMPLTEFQELMERMMAELFQELLGVTVPLPFRRIPYDEAMLRYGSDKPDLRFGLEIRDVT 309

Query: 304 DIVKDTEFKVFSSAVANGGVVKAINVKGGAGDYSRKDIDALGAFAANYGAKGLAWVKVEA 363
           ++VK   F+VF SAV +GG V+ +N KG  G +SRK+ID LGA A   GAKGLAW+ +  
Sbjct: 310 EMVKTCGFRVFQSAVNSGGAVRGLNAKG-CGGFSRKEIDDLGALAGQLGAKGLAWMVITE 368

Query: 364 DGVKGPIAKFFDEEKQSKLIEALDAAEGDLLLFGADQFEVVAASLGALRLKLGKERGLID 423
           +GVK PIAKFF +E+   +   L A  GDLLLF AD+ E+ A  LG LRL LG+  GLID
Sbjct: 369 EGVKSPIAKFFTDEELGAIRRELGAEVGDLLLFVADRPEICAEVLGQLRLHLGRRLGLID 428

Query: 424 EKLFNFLWVIDWPLLEHDPEEGRFYAAHHPFTMPVREDLELIETAPEDMKAQAYDLVLNG 483
           +  F F W+ D+PL  +D EE R+ A HHPFTMP  ED++L+E  P  ++AQAYD+VLNG
Sbjct: 429 DASFAFAWITDFPLFSYDEEEKRWVAEHHPFTMPRWEDVDLMERDPARVRAQAYDMVLNG 488

Query: 484 YELGGGSIRIFEKDIQEKMFALLGFSPEEAAEQFGFLLEAFEYGAPPHGGIALGLDRLVM 543
           YE+GGGS+RI+ +DIQE MF  LGFS +EA E+FGFLLEAFEYGAPPHGG+A G DR++M
Sbjct: 489 YEIGGGSLRIYRRDIQEAMFRTLGFSMDEAREKFGFLLEAFEYGAPPHGGMAFGFDRVIM 548

Query: 544 LLAGRTNLRDTIAFPKTASASCLMTEAPGEVSDAQLDELHL 584
           L+AG++++R+ IAFPKTASASCL+T AP ++SD Q  ELH+
Sbjct: 549 LMAGKSSIRECIAFPKTASASCLLTGAPSQISDEQWRELHI 589


Lambda     K      H
   0.319    0.137    0.394 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1027
Number of extensions: 51
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 592
Length of database: 598
Length adjustment: 37
Effective length of query: 555
Effective length of database: 561
Effective search space:   311355
Effective search space used:   311355
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis