Alignments for a candidate for proB in Arcobacter nitrofigilis DSM 7299

GapMind for Amino acid biosynthesis

Alignments for a candidate for proB in Arcobacter nitrofigilis DSM 7299

Align glutamate 5-kinase; EC 2.7.2.11 (characterized)
to candidate WP_013136150.1 ARNIT_RS11755 glutamate 5-kinase

Query= CharProtDB::CH_121311
         (251 letters)



>NCBI__GCF_000092245.1:WP_013136150.1
          Length = 254

 Score =  246 bits (628), Expect = 3e-70
 Identities = 128/253 (50%), Positives = 178/253 (70%), Gaps = 2/253 (0%)

Query: 1   MKRIVVKVGSHVISEENTLSFERLKNLVAFLAKLM--EKYEVILVTSAAISAGHTKLDID 58
           MKRIV+KVGS V+ E N L+ +RL NLV  +AKL   +KYE+ILV+S A++AG+  L++D
Sbjct: 1   MKRIVIKVGSAVLREGNVLAVDRLNNLVDLIAKLKAEKKYEIILVSSGAVAAGNITLELD 60

Query: 59  RKNLINKQVLAAIGQPFLISVYNELLAKFNKLGGQILLTGKDFDSRKATKHAKNAIDMMI 118
           R  ++N+Q LAAIGQP L+  Y +   +      Q+LL   DFDSRK +K+AK  +++++
Sbjct: 61  RTKVLNRQALAAIGQPLLMKHYKKRFREHGFTCAQMLLVANDFDSRKRSKNAKGVMEILL 120

Query: 119 NLGILPIINENDATAIEEIVFGDNDSLSAYATHFFDADLLVILSDIDGFYDKNPSEFSDA 178
              I+PI+NEND  A EE++ GDND L A+A  FFDAD+LVILSD+DG YD NP E  +A
Sbjct: 121 ENNIIPILNENDVIANEELLIGDNDQLGAHAAIFFDADMLVILSDVDGLYDCNPHENPEA 180

Query: 179 KRLEKITHIKEEWLQATIKTGSEHGTGGIVTKLKAAKFLLEHNKKMFLASGFDLSVAKTF 238
           K  + ++ +KE+ L+      SE  TGGIVTKLKAA  LL+++K M+L+SGFDL+ A  F
Sbjct: 181 KMRKIVSFLKEDELEMKYSPNSEFATGGIVTKLKAANLLLKNDKMMYLSSGFDLTNAYDF 240

Query: 239 LLEDKQIGGTLFE 251
           LL++    GTLF+
Sbjct: 241 LLKEDHKSGTLFK 253


Lambda     K      H
   0.319    0.136    0.373 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 228
Number of extensions: 16
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 251
Length of database: 254
Length adjustment: 24
Effective length of query: 227
Effective length of database: 230
Effective search space:    52210
Effective search space used:    52210
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 46 (22.3 bits)

Align candidate WP_013136150.1 ARNIT_RS11755 (glutamate 5-kinase)
to HMM TIGR01027 (proB: glutamate 5-kinase (EC 2.7.2.11))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR01027.hmm
# target sequence database:        /tmp/gapView.10095.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01027  [M=363]
Accession:   TIGR01027
Description: proB: glutamate 5-kinase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
    3.3e-71  226.3   0.2    3.6e-71  226.1   0.2    1.0  1  lcl|NCBI__GCF_000092245.1:WP_013136150.1  ARNIT_RS11755 glutamate 5-kinase


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000092245.1:WP_013136150.1  ARNIT_RS11755 glutamate 5-kinase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  226.1   0.2   3.6e-71   3.6e-71       1     252 [.       2     253 ..       2     254 .] 0.94

  Alignments for each domain:
  == domain 1  score: 226.1 bits;  conditional E-value: 3.6e-71
                                 TIGR01027   1 kriVvKlGsssLteesgklkrsklaelveqvaklkkaG.hevvivsSGavaaGleaLglperpkklaek 68 
                                               kriV+K+Gs++L e++  l+  +l++lv  +aklk++   e+++vsSGavaaG  +L l +  +k+ ++
  lcl|NCBI__GCF_000092245.1:WP_013136150.1   2 KRIVIKVGSAVLREGNV-LAVDRLNNLVDLIAKLKAEKkYEIILVSSGAVAAGNITLELDR--TKVLNR 67 
                                               69**********99988.****************9865169**************999975..467789 PP

                                 TIGR01027  69 QalaaVGQgrLmklyeklfsqyglkvaQiLLtradlskrerylNarntleellelgvvpivNENDtvav 137
                                               Qalaa+GQ  Lmk+y+k f ++g + aQ+LL ++d+ +r+r +Na+  +e lle++++pi+NEND +a 
  lcl|NCBI__GCF_000092245.1:WP_013136150.1  68 QALAAIGQPLLMKHYKKRFREHGFTCAQMLLVANDFDSRKRSKNAKGVMEILLENNIIPILNENDVIAN 136
                                               ********************************************************************* PP

                                 TIGR01027 138 eeikfGDNDtLsalvaalveAdlLvlltdvdgLydadprtnpdAklieeveeieeelkavagssgssvG 206
                                               ee+ +GDND+L a  a+  +Ad+Lv+l+dvdgLyd++p++np+Ak+ + v+ ++e+  +++ s  s++ 
  lcl|NCBI__GCF_000092245.1:WP_013136150.1 137 EELLIGDNDQLGAHAAIFFDADMLVILSDVDGLYDCNPHENPEAKMRKIVSFLKEDELEMKYSPNSEFA 205
                                               *************************************************************999999** PP

                                 TIGR01027 207 TGGmrtKleaaelAsragveviiasgekpekiadll..edaavgtlfe 252
                                               TGG+ tKl+aa+l  +    +++ sg +  +  d+l  ed++ gtlf+
  lcl|NCBI__GCF_000092245.1:WP_013136150.1 206 TGGIVTKLKAANLLLKNDKMMYLSSGFDLTNAYDFLlkEDHKSGTLFK 253
                                               ***************999999999999998888876335778888886 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (363 nodes)
Target sequences:                          1  (254 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01
# Mc/sec: 7.72
//
[ok]

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis