Align acetohydroxyacid synthase subunit B (EC 2.2.1.6) (characterized)
to candidate WP_013419888.1 RVAN_RS11500 acetolactate synthase 3 large subunit
Query= metacyc::MONOMER-18810 (585 letters) >NCBI__GCF_000166055.1:WP_013419888.1 Length = 587 Score = 637 bits (1644), Expect = 0.0 Identities = 317/571 (55%), Positives = 408/571 (71%), Gaps = 5/571 (0%) Query: 16 APEMIGAEILVHALAEEGVEYVWGYPGGAVLYIYDELHKQTKFEHILVRHEQAAVHAADG 75 A M GAEI++ ALA++GVE+++GYPGGAVL IYDE+ KQ +H LVRHEQ A HAA+G Sbjct: 2 ARAMTGAEIVLQALADQGVEHIFGYPGGAVLPIYDEIFKQNGIKHFLVRHEQGATHAAEG 61 Query: 76 YARATGKVGVALVTSGPGVTNAVTGIATAYLDSIPMVVITGNVPTHAIGQDAFQECDTVG 135 YAR++GKVGV LVTSGPG TNAVTG+A A +DSIP+V ITG V T IG DAFQECDTVG Sbjct: 62 YARSSGKVGVVLVTSGPGATNAVTGLADALMDSIPLVCITGQVGTPLIGSDAFQECDTVG 121 Query: 136 ITRPIVKHNFLVKDVRDLAATIKKAFFIAATGRPGPVVVDIPKDVSRNACKYEYPKSIDM 195 ITRP KHN+LVKDV DLA + +AF++A GRPGPVV+DIPKDV Y P + Sbjct: 122 ITRPCTKHNYLVKDVSDLARILHEAFYVAQNGRPGPVVIDIPKDVQFAKGTYIGPNKVVH 181 Query: 196 RSYNPVNKGHSGQIRKAVALLQGAERPYIYTGGGVVLAN--ASDELRQLAALTGHPVTNT 253 ++Y P KG IR AV L+ A++P YTGGGV+ + AS LR+ TG P+T+T Sbjct: 182 KTYRPQVKGSPQAIRDAVDLIASAKKPIFYTGGGVINSGPAASKLLREFVDTTGFPITST 241 Query: 254 LMGLGAFPGTSKQFVGMLGMHGTYEANMAMQNCDVLIAIGARFDDRVIGNPAHFTSQARK 313 LMGLGA+P +SKQ++GMLGMHGTYEANMAM +CDV+I +GARFDDR+ G F ++K Sbjct: 242 LMGLGAYPASSKQWLGMLGMHGTYEANMAMHDCDVMICVGARFDDRITGRLNAFAPNSKK 301 Query: 314 IIHIDIDPSSISKRVKVDIPIVGNVKDVLQELIAQIKASDIKPKREALAKWWEQIEQWRS 373 IHIDIDPSSISK V+ DIPI+G+ VL+E++ ++ + ++A+ WW I+ WRS Sbjct: 302 -IHIDIDPSSISKTVRADIPIIGDCGYVLEEMLRVWRSRAPQIDKKAIKAWWTSIDGWRS 360 Query: 374 VDCLKYDRSSEIIKPQYVVEKIWELTKG-DAFICSDVGQHQMWAAQFYKFDEPRRWINSG 432 DCLKY ++I PQY +++++ELTK D +I ++VGQHQMWAAQFY+FDEP W+ SG Sbjct: 361 RDCLKYKTRDDVIMPQYAIQRLYELTKNRDTYITTEVGQHQMWAAQFYRFDEPNHWMTSG 420 Query: 433 GLGTMGVGLPYAMGIKKAFPEKEVVTITGEGSIQMCIQELSTCLQYDTPVKICSLNNGYL 492 GLGTMG GLP A+G++ A PE V+ I GE S+ M +QE+ST QY P+KI LNN Y+ Sbjct: 421 GLGTMGYGLPAAVGVQVAHPESLVIDIAGESSVMMTMQEMSTAAQYGLPIKIFILNNEYM 480 Query: 493 GMVRQWQEIEYDNRYSHSYMDALPDFVKLAEAYGHVGMRVEKTSDVEPALREAFRLKDRT 552 GMVRQWQE+ + RYS SY +ALPDFVKLAEAY VG+R EK +++ + E + Sbjct: 481 GMVRQWQELMHGGRYSQSYAEALPDFVKLAEAYHGVGLRCEKPDELDDMIIEMIN-TPKP 539 Query: 553 VFLDFQTDPTENVWPMVQAGKGISEMLLGAE 583 V D + N +PM+ +G+ + ML G E Sbjct: 540 VLFDCRVSKLANCFPMIPSGEAHNNMLFGDE 570 Lambda K H 0.319 0.135 0.407 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 971 Number of extensions: 35 Number of successful extensions: 4 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 585 Length of database: 587 Length adjustment: 37 Effective length of query: 548 Effective length of database: 550 Effective search space: 301400 Effective search space used: 301400 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 53 (25.0 bits)
Align candidate WP_013419888.1 RVAN_RS11500 (acetolactate synthase 3 large subunit)
to HMM TIGR00118 (ilvB: acetolactate synthase, large subunit, biosynthetic type (EC 2.2.1.6))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00118.hmm # target sequence database: /tmp/gapView.18240.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00118 [M=557] Accession: TIGR00118 Description: acolac_lg: acetolactate synthase, large subunit, biosynthetic type Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 7.8e-247 806.1 0.0 9.1e-247 805.8 0.0 1.0 1 lcl|NCBI__GCF_000166055.1:WP_013419888.1 RVAN_RS11500 acetolactate syntha Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_000166055.1:WP_013419888.1 RVAN_RS11500 acetolactate synthase 3 large subunit # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 805.8 0.0 9.1e-247 9.1e-247 1 554 [. 5 566 .. 5 569 .. 0.98 Alignments for each domain: == domain 1 score: 805.8 bits; conditional E-value: 9.1e-247 TIGR00118 1 lkgaeilveslkkegvetvfGyPGGavlpiydaly.dselehilvrheqaaahaadGyarasGkvGvvl 68 ++gaei++++l ++gve++fGyPGGavlpiyd+++ +++++h lvrheq+a+haa+Gyar+sGkvGvvl lcl|NCBI__GCF_000166055.1:WP_013419888.1 5 MTGAEIVLQALADQGVEHIFGYPGGAVLPIYDEIFkQNGIKHFLVRHEQGATHAAEGYARSSGKVGVVL 73 79*********************************999******************************* PP TIGR00118 69 atsGPGatnlvtgiatayldsvPlvvltGqvatsliGsdafqeidilGitlpvtkhsflvkkaedlpei 137 +tsGPGatn+vtg+a+a +ds+Plv +tGqv t liGsdafqe+d +Git+p+tkh++lvk+++dl++i lcl|NCBI__GCF_000166055.1:WP_013419888.1 74 VTSGPGATNAVTGLADALMDSIPLVCITGQVGTPLIGSDAFQECDTVGITRPCTKHNYLVKDVSDLARI 142 ********************************************************************* PP TIGR00118 138 lkeafeiastGrPGPvlvdlPkdvteaeieleveekvelpgykptvkghklqikkaleliekakkPvll 206 l+eaf++a+ GrPGPv++d+Pkdv+ a+ ++ ++kv ++y+p+vkg+++ i+ a++li++akkP+ + lcl|NCBI__GCF_000166055.1:WP_013419888.1 143 LHEAFYVAQNGRPGPVVIDIPKDVQFAKGTYIGPNKVVHKTYRPQVKGSPQAIRDAVDLIASAKKPIFY 211 ********************************************************************* PP TIGR00118 207 vGgGviiae..aseelkelaerlkipvtttllGlGafpedhplalgmlGmhGtkeanlavseadlliav 273 GgGvi ++ as+ l+e+++++ p+t+tl+GlGa+p+ ++ lgmlGmhGt+ean+a++++d++i+v lcl|NCBI__GCF_000166055.1:WP_013419888.1 212 TGGGVINSGpaASKLLREFVDTTGFPITSTLMGLGAYPASSKQWLGMLGMHGTYEANMAMHDCDVMICV 280 ******885448999****************************************************** PP TIGR00118 274 GarfddrvtgnlakfapeakiihididPaeigknvkvdipivGdakkvleellkklkee....ekkeke 338 Garfddr+tg l+ fap++k ihididP++i+k+v++dipi+Gd+ vlee+l+ +++ +kk ++ lcl|NCBI__GCF_000166055.1:WP_013419888.1 281 GARFDDRITGRLNAFAPNSKKIHIDIDPSSISKTVRADIPIIGDCGYVLEEMLRVWRSRapqiDKKAIK 349 *****************************************************9887776767677777 PP TIGR00118 339 .WlekieewkkeyilkldeeeesikPqkvikelskllkd.eaivttdvGqhqmwaaqfyktkkprkfit 405 W+ +i+ w+++ +lk++++++ i Pq+ i++l++l+k+ ++++tt+vGqhqmwaaqfy++++p++++t lcl|NCBI__GCF_000166055.1:WP_013419888.1 350 aWWTSIDGWRSRDCLKYKTRDDVIMPQYAIQRLYELTKNrDTYITTEVGQHQMWAAQFYRFDEPNHWMT 418 8************************************9889**************************** PP TIGR00118 406 sgGlGtmGfGlPaalGakvakpeetvvavtGdgsfqmnlqelstiveydipvkivilnnellGmvkqWq 474 sgGlGtmG+GlPaa+G++va+pe+ v++++G++s++m++qe+st+++y++p+ki ilnne++Gmv+qWq lcl|NCBI__GCF_000166055.1:WP_013419888.1 419 SGGLGTMGYGLPAAVGVQVAHPESLVIDIAGESSVMMTMQEMSTAAQYGLPIKIFILNNEYMGMVRQWQ 487 ********************************************************************* PP TIGR00118 475 elfyeerysetklaselpdfvklaeayGvkgiriekpeeleeklkealeskepvlldvevdkeeevlPm 543 el++ +rys+++ ++ lpdfvklaeay +g+r ekp+el++ + e++++ +pvl+d +v k ++++Pm lcl|NCBI__GCF_000166055.1:WP_013419888.1 488 ELMHGGRYSQSYAEA-LPDFVKLAEAYHGVGLRCEKPDELDDMIIEMINTPKPVLFDCRVSKLANCFPM 555 *************95.***************************************************** PP TIGR00118 544 vapGagldelv 554 +++G + ++++ lcl|NCBI__GCF_000166055.1:WP_013419888.1 556 IPSGEAHNNML 566 ********997 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (557 nodes) Target sequences: 1 (587 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.04u 0.01s 00:00:00.05 Elapsed: 00:00:00.04 # Mc/sec: 7.76 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory