Alignments for a candidate for aspS2 in Calditerrivibrio nitroreducens DSM 19672

GapMind for Amino acid biosynthesis

Alignments for a candidate for aspS2 in Calditerrivibrio nitroreducens DSM 19672

Align Aspartate--tRNA(Asp/Asn) ligase; EC 6.1.1.23 (characterized, see rationale)
to candidate WP_013450995.1 CALNI_RS04355 aspartate--tRNA ligase

Query= uniprot:SYDND_DESVH
         (610 letters)



>NCBI__GCF_000183405.1:WP_013450995.1
          Length = 596

 Score =  696 bits (1795), Expect = 0.0
 Identities = 334/586 (56%), Positives = 430/586 (73%), Gaps = 4/586 (0%)

Query: 19  LGDWQRTHSCCELTAADVGNDVCIMGWVQYRRDHGGLIFVDLRDRKGLTQVVFSPDFAPE 78
           LGDW+RTH C EL   + G D+C+MGWV  RRDHGG+IF+DLRD+ GL Q+V +P+ +PE
Sbjct: 5   LGDWRRTHYCGELNGKNTGEDICVMGWVHRRRDHGGVIFIDLRDKTGLLQLVLNPEISPE 64

Query: 79  AHKDAHIVRSEYVLAIRGRVRPRPEGMTNPGMKTGEIEVVVSEWKLLNTSKTPPFLIEDR 138
           AH  A  VRSEYV+A++G+V  RPEG  N  + TGEIE++V E K+LN +KTPPF IE+ 
Sbjct: 65  AHSIAEAVRSEYVVAVKGKVEKRPEGTINENLPTGEIEIIVKEIKILNNAKTPPFQIEEN 124

Query: 139 TEASENLRLAWRYLDLRRPRMARNFMLRHRAAQSARRYLDELDFLEIETPYLTKATPEGA 198
           T   E++RL +RYLDLRRP + +N +LRH   ++ R YL    FL+IETP+LTK+TPEGA
Sbjct: 125 TNVGEDVRLKYRYLDLRRPNLQKNIILRHNLTRTMREYLYGKGFLDIETPFLTKSTPEGA 184

Query: 199 RDFLVPSRLNHGMFYALPQSPQIFKQLLMVSGMDRYYQIVRCFRDEDMRADRQLEFTQID 258
           RD+LVPSR+N G FYALPQSPQ+FKQLLM+SG DRY+QIVRCFRDED+RADRQ EFTQ+D
Sbjct: 185 RDYLVPSRVNPGKFYALPQSPQMFKQLLMISGYDRYFQIVRCFRDEDLRADRQPEFTQLD 244

Query: 259 IEMSFVDEERVMSMAEGLMSRVMKDTLGVDVTVPFPRMTYDQAMGEYGVDKPDTRFDLRL 318
           +EMSF+D + +M + EGL   + +   G+ V  PFP MTY +AM +Y  D PDTRF+L  
Sbjct: 245 MEMSFIDRQDLMDLIEGLFVEIFQKVAGITVQRPFPVMTYAEAMEKYSHDAPDTRFELFT 304

Query: 319 KDVTDAVRGSEFKLFAKA----PLVKAMRVPGGETMTRKEIDEFTEFVKIYGAQGLAWIK 374
           K + D V+  EFK+F  A     +VKA+   G    +RKEIDE T++    GA+GLA+IK
Sbjct: 305 KTINDLVKDCEFKVFTDAIKEGGIVKAINAKGAGKFSRKEIDEITDYAVSLGAKGLAYIK 364

Query: 375 IREGEWQSPIAKFLSEAERAALVAALGLEVGDIVFFQAGEPGMVNAALGNLRVKLGQHLG 434
           I E   QSPI KFL E     ++  +  +VGDIVFF AGE   VN  +  +R++LG+ LG
Sbjct: 365 INEDGLQSPIIKFLGEQLTHRILDTMDAKVGDIVFFGAGEKSTVNLFMSKVRLELGRRLG 424

Query: 435 LIPEDTYNFLWVTDFPLFEYDEEEKRYVACHHPFTSPKDGHFDLMTSDPAAARARAYDMV 494
           LI ++ YNF WV DFPL E+D+EEKRY A HHPFT+P D    L  +DP   RA+AYD+V
Sbjct: 425 LIDKNKYNFTWVIDFPLLEWDKEEKRYAAVHHPFTAPVDEDIPLFDTDPLKIRAKAYDLV 484

Query: 495 LNGYELGGGSIRIHSAEVQRRMFAALGLDPQEAEEKFGFLIQALEHGAPPHGGIAFGMDR 554
           LNG E+GGGSIRIH +++Q +MF  LGL  +E + KFGF I+AL +G PPHGGIAFG+DR
Sbjct: 485 LNGSEIGGGSIRIHRSDIQEKMFNILGLTKEECDLKFGFFIEALRYGTPPHGGIAFGVDR 544

Query: 555 LVMLLTGSPSIRDVIAFPKTQKATCLMTQAPDAVSARQLRDLGIRL 600
           +  +L G+ SIRDVIAFPKTQKATCLM+ AP+ V A+QL++L I+L
Sbjct: 545 IASILAGADSIRDVIAFPKTQKATCLMSDAPNKVDAKQLKELSIKL 590


Lambda     K      H
   0.322    0.138    0.415 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1027
Number of extensions: 30
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 610
Length of database: 596
Length adjustment: 37
Effective length of query: 573
Effective length of database: 559
Effective search space:   320307
Effective search space used:   320307
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis