Alignments for a candidate for leuA in Sulfuricurvum kujiense DSM 16994

GapMind for Amino acid biosynthesis

Alignments for a candidate for leuA in Sulfuricurvum kujiense DSM 16994

Align 2-isopropylmalate synthase; Alpha-IPM synthase; Alpha-isopropylmalate synthase; EC 2.3.3.13 (characterized)
to candidate WP_013461086.1 SULKU_RS11215 2-isopropylmalate synthase

Query= SwissProt::P42455
         (616 letters)



>NCBI__GCF_000183725.1:WP_013461086.1
          Length = 555

 Score =  553 bits (1424), Expect = e-162
 Identities = 282/566 (49%), Positives = 375/566 (66%), Gaps = 22/566 (3%)

Query: 40  RYMPFEVEVEDISLPDRTWPDKKITVAPQWCAVDLRDGNQALIDPMSPERKRRMFELLVQ 99
           +Y P+      + LP+R WP K IT AP WC+VDLRDGNQALI PM+ ++K  +F LL++
Sbjct: 8   KYRPYP----QVDLPNRIWPTKTITHAPIWCSVDLRDGNQALITPMNLDQKLSLFSLLLK 63

Query: 100 MGFKEIEVGFPSASQTDFDFVREIIEKGMIPDDVTIQVLVQAREHLIRRTFEACEGAKNV 159
           +GFK IEVGFPSAS+ +FDF+R ++E+ +IPDDVT+QVLVQAREHLI +TFEA  G K  
Sbjct: 64  LGFKNIEVGFPSASKVEFDFLRTLVEQKLIPDDVTVQVLVQAREHLIDKTFEALAGVKKA 123

Query: 160 IVHFYNSTSILQRNVVFRMDKVQVKKLATDAAELIKTIAQDYPDTNWRWQYSPESFTGTE 219
           IVH YNSTS+ QR +VF  ++ ++  LA +  +++K  A  + D     +YSPESFTGTE
Sbjct: 124 IVHLYNSTSVAQRKIVFGKEQDEIIALALEGVDMVKERAAKH-DGEIMLEYSPESFTGTE 182

Query: 220 VEYAKEVVDAVVEVMDPTPENPMIINLPSTVEMITPNVYADSIEWMHRNLNRRDSIILSL 279
           +E+A  + +AV +    + E  ++INLP+TVEM TPNVYAD IEWM R+L  R+ +++S 
Sbjct: 183 LEFAARICNAVTDRWGISAERKVVINLPATVEMATPNVYADQIEWMSRHLTNREHVLIST 242

Query: 280 HPHNDRGTGVGAAELGYMAGADRIEGCLFGNGERTGNVCLVTLALNMLTQGVDPQLDFTD 339
           H HNDRGT V A EL  +AGADR+EG L  NGERTGNV ++TLALNM TQGVDP+LDF+D
Sbjct: 243 HTHNDRGTSVAATELALLAGADRVEGTLLSNGERTGNVDIITLALNMYTQGVDPELDFSD 302

Query: 340 IRQIRSTVEYCNQLRVPERHPYGGDLVFTAFSGSHQDAVNKGLDAMAAKVQPGASSTEVS 399
           +  +   VE C  ++  ERHPY G+LV+TAFSGSHQDA+NKGL    +K           
Sbjct: 303 LETVVRVVEECTDMKTHERHPYVGELVYTAFSGSHQDAINKGLAYQRSK----------- 351

Query: 400 WEQLRDTEWEVPYLPIDPKDVGRDYEAVIRVNSQSGKGGVAYIMKTDHGLQIPRSMQVEF 459
                D  WEVPYLPIDP+DVGR+YE +IR+NSQSGKGGVAY+++   G  +P+ M  E 
Sbjct: 352 ----EDPFWEVPYLPIDPQDVGRNYEGIIRINSQSGKGGVAYVLEDKFGYSLPKKMHPEI 407

Query: 460 STVVQNVTDAEGGEVNSKAMWDIFATEYLERTAPVEQIALRVENAQTENEDASITAELIH 519
             +VQ VTD  G E+ S+ + DIF   Y      +E +   V +   +   A    E  H
Sbjct: 408 GRIVQQVTDEAGRELTSEEILDIFEKTYFGEPHDIEFVDYSVISESAKEHSAKCVLEYTH 467

Query: 520 NGKDVTVDGRGNGPLAAYANAL-EKLGIDVEIQEYNQHARTSGDDAEAAAYVLAEVNG-R 577
           NGK +  +G GNGP+ A  NAL  +   + +I  Y++H+R     AEA AY+  +     
Sbjct: 468 NGKAIRSEGVGNGPIDACKNALMVEYSHNFKILSYSEHSRGELSSAEAVAYIEIQTESLE 527

Query: 578 KVWGVGIAGSITYASLKAVTSAVNRA 603
           K +GVG    IT AS+KA+ SA+NRA
Sbjct: 528 KFFGVGCDNDITVASIKAMFSALNRA 553


Lambda     K      H
   0.316    0.133    0.390 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 875
Number of extensions: 31
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 616
Length of database: 555
Length adjustment: 37
Effective length of query: 579
Effective length of database: 518
Effective search space:   299922
Effective search space used:   299922
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 53 (25.0 bits)

Align candidate WP_013461086.1 SULKU_RS11215 (2-isopropylmalate synthase)
to HMM TIGR00970 (leuA: 2-isopropylmalate synthase (EC 2.3.3.13))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00970.hmm
# target sequence database:        /tmp/gapView.9533.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00970  [M=564]
Accession:   TIGR00970
Description: leuA_yeast: 2-isopropylmalate synthase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
   5.9e-227  740.6   0.0   6.7e-227  740.4   0.0    1.0  1  lcl|NCBI__GCF_000183725.1:WP_013461086.1  SULKU_RS11215 2-isopropylmalate 


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000183725.1:WP_013461086.1  SULKU_RS11215 2-isopropylmalate synthase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  740.4   0.0  6.7e-227  6.7e-227       3     562 ..       7     553 ..       5     555 .] 0.96

  Alignments for each domain:
  == domain 1  score: 740.4 bits;  conditional E-value: 6.7e-227
                                 TIGR00970   3 kkykpfkaiklsnrkwpdkvitraprwlsvdlrdGnqalidpmsverkkryfkllvriGfkeievgfps 71 
                                                ky+p+  + l+nr wp k it+ap w+svdlrdGnqali pm+ ++k  +f+ll+++Gfk+ievgfps
  lcl|NCBI__GCF_000183725.1:WP_013461086.1   7 GKYRPYPQVDLPNRIWPTKTITHAPIWCSVDLRDGNQALITPMNLDQKLSLFSLLLKLGFKNIEVGFPS 75 
                                               59******************************************************************* PP

                                 TIGR00970  72 asqtdfdfvreiieqglipddvtiqvltqsreelikrtvealsGakkaivhlynatsdlfrevvfrasr 140
                                               as+ +fdf r ++eq lipddvt+qvl+q+re+li++t+eal+G kkaivhlyn+ts  +r++vf +++
  lcl|NCBI__GCF_000183725.1:WP_013461086.1  76 ASKVEFDFLRTLVEQKLIPDDVTVQVLVQAREHLIDKTFEALAGVKKAIVHLYNSTSVAQRKIVFGKEQ 144
                                               ********************************************************************* PP

                                 TIGR00970 141 eevlalavegsklvrklvkdaaksketrwsfeyspesfsdtelefavevceavkeviepteerpiifnl 209
                                               +e++ala+eg      +vk+ a +++ +   eyspesf++telefa ++c+av + +  ++er ++ nl
  lcl|NCBI__GCF_000183725.1:WP_013461086.1 145 DEIIALALEG----VDMVKERAAKHDGEIMLEYSPESFTGTELEFAARICNAVTDRWGISAERKVVINL 209
                                               **********....456677777888999**************************************** PP

                                 TIGR00970 210 patvevatpnvyadsieylstniaerekvilslhphndrGtavaaaelGllaGadrieGclfGnGertG 278
                                               patve+atpnvyad+ie++s+++++re+v++s+h+hndrGt+vaa+el llaGadr+eG l+ nGertG
  lcl|NCBI__GCF_000183725.1:WP_013461086.1 210 PATVEMATPNVYADQIEWMSRHLTNREHVLISTHTHNDRGTSVAATELALLAGADRVEGTLLSNGERTG 278
                                               ********************************************************************* PP

                                 TIGR00970 279 nvdlvtlalnlytqGvspnldfsdldeilrvvercnkipvherhpygGdlvvtafsGshqdaikkGlda 347
                                               nvd++tlaln+ytqGv+p+ldfsdl+ ++rvve+c  ++ herhpy G+lv+tafsGshqdai+kGl  
  lcl|NCBI__GCF_000183725.1:WP_013461086.1 279 NVDIITLALNMYTQGVDPELDFSDLETVVRVVEECTDMKTHERHPYVGELVYTAFSGSHQDAINKGLAY 347
                                               *******************************************************************87 PP

                                 TIGR00970 348 ldkkkaaadtlwkvpylpldpkdvgreyeavirvnsqsGkGGvayvlktdlGldlprrlqiefssvvkd 416
                                               +  k    d+ w+vpylp+dp+dvgr+ye +ir+nsqsGkGGvayvl  + G +lp+++  e +++v++
  lcl|NCBI__GCF_000183725.1:WP_013461086.1 348 QRSK---EDPFWEVPYLPIDPQDVGRNYEGIIRINSQSGKGGVAYVLEDKFGYSLPKKMHPEIGRIVQQ 413
                                               6554...468*********************************************************** PP

                                 TIGR00970 417 iadskGkelsskeisdlfkeeyllnveqlerislvdyaveddGteskvitavvkikgekkdi..eGsGn 483
                                               ++d  G+el+s+ei d+f+++y+    +++ i+ vdy+v ++ +++++ ++v++   + k i  eG+Gn
  lcl|NCBI__GCF_000183725.1:WP_013461086.1 414 VTDEAGRELTSEEILDIFEKTYFG---EPHDIEFVDYSVISESAKEHSAKCVLEYTHNGKAIrsEGVGN 479
                                               **********************99...678899*******************9986555554339**** PP

                                 TIGR00970 484 GplsalvdaladllnvdvavadysehalgsGddakaasyvelsvrrasdaekatvwGvGiaedvtsasl 552
                                               Gp+ a  +al   +  +++++ yseh+ g  ++a+a +y+e+  +        +++GvG ++d+t as+
  lcl|NCBI__GCF_000183725.1:WP_013461086.1 480 GPIDACKNALMVEYSHNFKILSYSEHSRGELSSAEAVAYIEIQTESLE-----KFFGVGCDNDITVASI 543
                                               *****************************************9876655.....79************** PP

                                 TIGR00970 553 ravlsavnra 562
                                               +a++sa+nra
  lcl|NCBI__GCF_000183725.1:WP_013461086.1 544 KAMFSALNRA 553
                                               ********98 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (564 nodes)
Target sequences:                          1  (555 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.03u 0.01s 00:00:00.04 Elapsed: 00:00:00.03
# Mc/sec: 9.20
//
[ok]

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis