Alignments for a candidate for metH in Thermovibrio ammonificans HB-1

GapMind for Amino acid biosynthesis

Alignments for a candidate for metH in Thermovibrio ammonificans HB-1

Align vitamin B12-dependent methionine synthase without a reactivation domain (EC 2.1.1.13) (characterized)
to candidate WP_013537367.1 THEAM_RS03095 homocysteine S-methyltransferase

Query= reanno::Miya:8499710
         (818 letters)



>NCBI__GCF_000185805.1:WP_013537367.1
          Length = 841

 Score =  616 bits (1588), Expect = e-180
 Identities = 364/838 (43%), Positives = 503/838 (60%), Gaps = 45/838 (5%)

Query: 15  VFDGGMGTMLQSRGLPPGVSPELFCLARPDVLVGIHADYLRAGADVLTTNTFGGCIHKLG 74
           V DGGMGTML ++G+    +PEL  + +P+VL  IH++Y+ AGAD++ TNTFG    KL 
Sbjct: 14  VLDGGMGTMLMAKGVDVNFAPELLNVEKPEVLKEIHSEYVEAGADIIETNTFGSNRIKLS 73

Query: 75  TGPGAPDVVEFNRAMARAAREAVLASGREAFVAGSVGPSGHFMRPLGDLDPAELVAAFRA 134
                  V E   A  + A+EA  A GR A VA SVGP+G F  P+GD    ELV  F+ 
Sbjct: 74  HYGLENRVKELTAAGVKLAKEA--ARGR-ALVALSVGPTGVFAEPVGDYTFDELVDVFKE 130

Query: 135 QIRGLVQGGVDLILAETQFDLAEARAIVLAARAECDLPVGVSMTF-ENGVSLTGTRPEVF 193
           QI    + G DL+L ET  D+ EA+A V AAR  CDLPV VSMT+ E+G +L GT PEV 
Sbjct: 131 QIEAGAEAGADLVLIETMSDIKEAKAAVFAAREVCDLPVLVSMTYQEDGRTLLGTPPEVA 190

Query: 194 VQSMLNMGVDLVGTNCSAGPEQMAEVADELLAISEVPVLVEPNAGLPELVDGKTVFRLGP 253
                   V  VG NCS GPE   E+     +++  P++V  NAGLP L +GKTV+   P
Sbjct: 191 AAVFEGFNVAAVGANCSLGPESFVEIIKRTASVTTTPIIVYANAGLPVLENGKTVYPEPP 250

Query: 254 DDFARHTARFAASGVRMLGGCCGTTPDHIAALRGALDNLSGGLVPDPARRD---GIVLTT 310
           + F ++   F  +G  ++GGCCGTTPDHI A++ A++ L       P  R+   G+ + +
Sbjct: 251 ETFEKYAVEFVKAGANIIGGCCGTTPDHIRAIKRAVEGLK------PVERNPVKGVKVAS 304

Query: 311 RAQAVHIGAGSPIRIIGERINPTGKKLLTAELQAGEFAQALRFADEQVEAGAPLLDVNVG 370
           R + V IG G P RIIGERINPTGKK L   L+A +F+   + A +QVE GA LLDVNVG
Sbjct: 305 RTKLVLIGTGHPTRIIGERINPTGKKKLQEALKAKDFSLVKQEAKKQVEEGADLLDVNVG 364

Query: 371 APMVDEAVLLPALVERLVARHSLPLSLDSSNADAIAAALPFHPGSPLVNSISGEPGRMEH 430
            P  DE  L+   V+ ++    +PL +D+ +  A+  AL    G P+VNS+SGE   +E 
Sbjct: 365 VPGADEPSLMREAVKTVMEAVDVPLVIDTKDPKAVEEALKMCDGRPVVNSVSGEKKDVEQ 424

Query: 431 LGPLCRDHGAPFILLPLKGRKLPVTAAERIAIIEELLQQADSLRIPRRLVMVDVLALAVS 490
           + P+   +GA  +LL +    L   A ER+ IIE LL++ + + + R   + DVL LAVS
Sbjct: 425 ILPVAAKYGANVLLLAIDDEGLKEKAQERVEIIERLLKECEKVGVDRSSTVADVLNLAVS 484

Query: 491 SKAEAARHCLDTIRWCAAQ-GLPTTIGLSNISFGLPARELLNSTFLAMAAGAGLSSCIAH 549
           +  ++    L  IR    + G+ TT+G+SN+SFGLP+R L+NS F+AMA  AGL S I +
Sbjct: 485 AMPDSTVETLKAIRLVKERFGIATTLGVSNVSFGLPSRSLINSAFMAMAIYAGLDSGIVN 544

Query: 550 PGNARIREAVAASSVLLGLDANAESFIEGYSGWTPGGDATGATPA-----GVAGGTGGGT 604
           PG++R+ E + AS VL+G D  AE F+  +  ++P G+      A      +AG   G  
Sbjct: 545 PGDSRMVETIFASDVLVGKDRGAERFVSRFQNYSPKGEDAECRKALERICQIAGAVLGTV 604

Query: 605 -------GGVKAKAATLEEA----------------VIRGDREGALALVERALSEGADPF 641
                     KAK  T EEA                V+ GDREG +   E AL E  DP 
Sbjct: 605 PQAFSHEAEEKAKGETTEEASDSEAPAGILGTIFKKVLEGDREGIVGPTEEALKE-FDPV 663

Query: 642 SLVQEKLIPGITEVGRRYERREYFLPQLIRSAETMQHAFRKLQPLLEAQRGH-EARPVII 700
            +    LIP +  VG+R+E+ E FLPQ++RSA+ +Q AF  L+  ++ + G+ +    I+
Sbjct: 664 EVSDRALIPALDVVGKRFEKGEIFLPQMLRSAQAVQAAFEVLKREMKKKGGNLKLGGKIV 723

Query: 701 MATVEGDIHDIGKNIVTLMLGNHGFDVVDLGKDVKAADIVEAAERHGARVIGLSALMTTT 760
           MATV GD+H+IGKNIV  ML N GFDV+DLG +V  A++V AA+ H A ++GLSALMTTT
Sbjct: 724 MATVHGDVHEIGKNIVITMLENSGFDVIDLGTNVPPAEVVRAAKEHNADIVGLSALMTTT 783

Query: 761 MVRMEDTVRLVRERGLPVKVMVGGAVVTPAYAEAIGADGYSADAVEAVRVAKELLTVQ 818
           +  ME+T++ +R+ G+ V V+VGGAVVTP YAE+IG   Y  DA EAV++ K+LL V+
Sbjct: 784 LPAMEETIKALRDAGVEVPVIVGGAVVTPEYAESIGGI-YGGDAQEAVKIVKKLLKVE 840


Lambda     K      H
   0.320    0.137    0.395 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1643
Number of extensions: 71
Number of successful extensions: 8
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 818
Length of database: 841
Length adjustment: 42
Effective length of query: 776
Effective length of database: 799
Effective search space:   620024
Effective search space used:   620024
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 56 (26.2 bits)

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis