Alignments for a candidate for hisD in Nitratifractor salsuginis DSM 16511

GapMind for Amino acid biosynthesis

Alignments for a candidate for hisD in Nitratifractor salsuginis DSM 16511

Align histidinol dehydrogenase (EC 1.1.1.23) (characterized)
to candidate WP_013554303.1 NITSA_RS06905 histidinol dehydrogenase

Query= BRENDA::Q8G2R2
         (430 letters)



>NCBI__GCF_000186245.1:WP_013554303.1
          Length = 431

 Score =  353 bits (905), Expect = e-102
 Identities = 192/421 (45%), Positives = 262/421 (62%), Gaps = 1/421 (0%)

Query: 9   DPDFEQKFAAFLSGKREVSEDVDRAVREIVDRVRREGDSALLDYSRRFDRID-LEKTGIA 67
           D  F + F   L       + V   V  ++  +R EG++AL  +  RFDR +  +   + 
Sbjct: 8   DERFPEIFEELLGRGAMDIDQVTETVAALLKEIREEGNAALRRHIARFDRWEPADDAALQ 67

Query: 68  VTEAEIDAAFDAAPASTVEALKLARDRIEKHHARQLPKDDRYTDALGVELGSRWTAIEAV 127
           V  AE+  A++A      +AL LA DRI+ +H + LPK     +  G  LG + T ++  
Sbjct: 68  VDPAEMAMAYEAIAPELRDALHLAYDRIKAYHEKLLPKSWFEYEKNGSMLGQKVTPVDRA 127

Query: 128 GLYVPGGTASYPSSVLMNAMPAKVAGVDRIVMVVPAPDGNLNPLVLVAARLAGVSEIYRV 187
           GLY+PGG A+YPSS+LMNA+PA VAGV+ IV+  P PD  +N L+L A  L G+ ++++V
Sbjct: 128 GLYIPGGKAAYPSSLLMNAVPAIVAGVEEIVVCTPTPDNEINVLLLAACHLCGIKKVFKV 187

Query: 188 GGAQAIAALAYGTETIRPVAKIVGPGNAYVAAAKRIVFGTVGIDMIAGPSEVLIVADKDN 247
           GGA AI A+AYGTETI  V  I GPGN +VA AK++V+G V IDMIAGPSE+ I+AD   
Sbjct: 188 GGASAIGAMAYGTETIPKVDVITGPGNIFVATAKKLVYGEVNIDMIAGPSEIGILADGSA 247

Query: 248 NPDWIAADLLAQAEHDTAAQSILMTNDEAFAHAVEEAVERQLHTLARTETASASWRDFGA 307
            PDW+A DLL+QAEHD  A SIL+T+D   A  V + VE+ L  L R   A  S  + GA
Sbjct: 248 RPDWVAMDLLSQAEHDEMASSILITDDAELAKRVVDEVEKALSKLEREAIARKSIEERGA 307

Query: 308 VILVKDFEDAIPLANRIAAEHLEIAVADAEAFVPRIRNAGSIFIGGYTPEVIGDYVGGCN 367
           +I+  D  +AI L N+IA EHLE+  A     +  I++AG+IF+G YTPE IGDY+ G N
Sbjct: 308 IIVTSDMAEAIDLMNQIAPEHLEVMTASPMELLGSIKHAGAIFLGHYTPEPIGDYIAGPN 367

Query: 368 HVLPTARSARFSSGLSVLDYMKRTSLLKLGSEQLRALGPAAIEIARAEGLDAHAQSVAIR 427
           H LPT  +A+F S LSV  ++K++SL+ +    +R LG A   +A  EGL AH  SV IR
Sbjct: 368 HTLPTGGTAKFYSPLSVEHFLKKSSLIMMTEAGVRELGDACALLAHTEGLTAHEASVRIR 427

Query: 428 L 428
           L
Sbjct: 428 L 428


Lambda     K      H
   0.318    0.134    0.375 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 416
Number of extensions: 15
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 430
Length of database: 431
Length adjustment: 32
Effective length of query: 398
Effective length of database: 399
Effective search space:   158802
Effective search space used:   158802
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 51 (24.3 bits)

Align candidate WP_013554303.1 NITSA_RS06905 (histidinol dehydrogenase)
to HMM TIGR00069 (hisD: histidinol dehydrogenase (EC 1.1.1.23))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00069.hmm
# target sequence database:        /tmp/gapView.19888.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00069  [M=393]
Accession:   TIGR00069
Description: hisD: histidinol dehydrogenase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
   3.1e-169  549.3   0.3   3.5e-169  549.1   0.3    1.0  1  lcl|NCBI__GCF_000186245.1:WP_013554303.1  NITSA_RS06905 histidinol dehydro


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000186245.1:WP_013554303.1  NITSA_RS06905 histidinol dehydrogenase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  549.1   0.3  3.5e-169  3.5e-169       1     393 []      33     427 ..      33     427 .. 0.99

  Alignments for each domain:
  == domain 1  score: 549.1 bits;  conditional E-value: 3.5e-169
                                 TIGR00069   1 vkeiiedvrkeGdeAlleytekfdkv...kleslrvseeeleealeavdeelkealelaaeniekfhek 66 
                                               v+++++++r+eG++Al+++  +fd++   + ++l+v  +e++ a+ea+ +el++al+la+++i+++hek
  lcl|NCBI__GCF_000186245.1:WP_013554303.1  33 VAALLKEIREEGNAALRRHIARFDRWepaDDAALQVDPAEMAMAYEAIAPELRDALHLAYDRIKAYHEK 101
                                               67899********************97666679************************************ PP

                                 TIGR00069  67 qlpesveveteegvllgqkvrplervglYvPgGkaaypStvlmtavpAkvAgvkeivvvtPpkkdgkvn 135
                                                lp+s++  +++g++lgqkv+p++r+glY+PgGkaaypS++lm+avpA vAgv+eivv+tP+  d+++n
  lcl|NCBI__GCF_000186245.1:WP_013554303.1 102 LLPKSWFEYEKNGSMLGQKVTPVDRAGLYIPGGKAAYPSSLLMNAVPAIVAGVEEIVVCTPTP-DNEIN 169
                                               **************************************************************6.999** PP

                                 TIGR00069 136 pavlaaakllgvdevykvGGaqaiaalayGtetvpkvdkivGPGniyVtaAKklvfgevgidmiaGPsE 204
                                                 +laa++l+g+++v+kvGGa+ai+a+ayGtet+pkvd+i+GPGni+V++AKklv+gev+idmiaGPsE
  lcl|NCBI__GCF_000186245.1:WP_013554303.1 170 VLLLAACHLCGIKKVFKVGGASAIGAMAYGTETIPKVDVITGPGNIFVATAKKLVYGEVNIDMIAGPSE 238
                                               ********************************************************************* PP

                                 TIGR00069 205 vlviadesanpelvaaDllsqaEHdedaqailvttseelaekveeeveeqleelerkeiaeksleknga 273
                                               + ++ad sa p++va+DllsqaEHde a++il+t+++ela++v +eve+ l++ler+ ia+ks+e++ga
  lcl|NCBI__GCF_000186245.1:WP_013554303.1 239 IGILADGSARPDWVAMDLLSQAEHDEMASSILITDDAELAKRVVDEVEKALSKLEREAIARKSIEERGA 307
                                               ********************************************************************* PP

                                 TIGR00069 274 iilvddleealelsneyApEHLelqtkdpeellkkiknaGsvflGeytpealgdyvaGpnhvLPTsgtA 342
                                               ii+++d++ea++l+n++ApEHLe++t++p+ell +ik+aG++flG+ytpe++gdy+aGpnh+LPT+gtA
  lcl|NCBI__GCF_000186245.1:WP_013554303.1 308 IIVTSDMAEAIDLMNQIAPEHLEVMTASPMELLGSIKHAGAIFLGHYTPEPIGDYIAGPNHTLPTGGTA 376
                                               ********************************************************************* PP

                                 TIGR00069 343 rfasglsvedFlkrisvqelskealeelaeaveklaeaEgLeaHaeavevR 393
                                               +f+s+lsve+Flk++s++ +++++++el++a++ la++EgL+aH+++v++R
  lcl|NCBI__GCF_000186245.1:WP_013554303.1 377 KFYSPLSVEHFLKKSSLIMMTEAGVRELGDACALLAHTEGLTAHEASVRIR 427
                                               *************************************************99 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (393 nodes)
Target sequences:                          1  (431 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01
# Mc/sec: 11.33
//
[ok]

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis