Align Shikimate kinase; SK; EC 2.7.1.71 (uncharacterized)
to candidate WP_013554882.1 NITSA_RS09825 shikimate kinase
Query= curated2:Q7VIH7 (167 letters) >NCBI__GCF_000186245.1:WP_013554882.1 Length = 191 Score = 107 bits (267), Expect = 1e-28 Identities = 63/168 (37%), Positives = 98/168 (58%), Gaps = 6/168 (3%) Query: 2 ENIVLIGFMGSGKTTIGREIALLGGRFLLDTDQIIEQNMGKSINEIFESVGESGFRRIES 61 +NI+LIGFMG GK T R A G + +DTD +IE +SI IF+ GE+ FRR+E Sbjct: 15 KNIILIGFMGVGKGTTARAYAKKYGIYNIDTDDLIESKENRSIPRIFKKKGEAYFRRLEQ 74 Query: 62 QLILWLSANVKNAVIATGGGMPIYNDVAYLGYVFWLDMSFESILKRLTITEQ-----EKR 116 + W+ V +I+ GGG +++ LG V LD S++ I RL+ + KR Sbjct: 75 ETADWIECCVDGTLISCGGGFYQVDNLKKLGTVVLLDASYDWIYHRLSSAKNAKKKLAKR 134 Query: 117 PLFSDISKARQLYNERKSIYKKQSKYIINGDASAL-EIARKIIECMDK 163 PLFS+ KA++LY +R+ Y+K + I+N + ++ EI +I E +++ Sbjct: 135 PLFSEPKKAKKLYEKREKAYRKVADLIVNVEGKSMDEILTEIHEGVEQ 182 Lambda K H 0.319 0.137 0.383 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 90 Number of extensions: 2 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 167 Length of database: 191 Length adjustment: 19 Effective length of query: 148 Effective length of database: 172 Effective search space: 25456 Effective search space used: 25456 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 44 (21.6 bits)
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory