Alignments for a candidate for leuA in Methanobacterium lacus AL-21

GapMind for Amino acid biosynthesis

Alignments for a candidate for leuA in Methanobacterium lacus AL-21

Align Probable 2-isopropylmalate synthase; EC 2.3.3.13; Alpha-IPM synthase; Alpha-isopropylmalate synthase (uncharacterized)
to candidate WP_013645928.1 METBO_RS11680 (R)-citramalate synthase

Query= curated2:Q8TYB1
         (499 letters)



>NCBI__GCF_000191585.1:WP_013645928.1
          Length = 501

 Score =  532 bits (1371), Expect = e-156
 Identities = 269/490 (54%), Positives = 365/490 (74%), Gaps = 5/490 (1%)

Query: 4   RVRIFDTTLRDGEQTPGVSLTVEEKVEIARKLDEFGVDTIEAGFPVASEGEFEAVRAIAG 63
           + RIFDTTLRDGEQTPGVSLT ++K+ IA KLD  GVD IEAG  + S GE E +++I  
Sbjct: 2   KARIFDTTLRDGEQTPGVSLTPDQKLRIAVKLDNLGVDVIEAGSAITSSGEREGIKSIVS 61

Query: 64  EELDAEICGLARCVKGDIDAAIDADVDCVHVFIATSDIHLRYKLEMSREEALERAIEGVE 123
           E L AEIC  AR ++ D+DAA++  VD VH+ + TSD+H+ +KL  +REE    AI+ VE
Sbjct: 62  EGLSAEICSFARAMRIDVDAALECGVDSVHLVVPTSDLHIEHKLRKTREEVKATAIDAVE 121

Query: 124 YASDHGVTVEFSAEDATRTDRDYLLEVYKATVEAGADRVNVPDTVGVMTPPEMYRLTAEV 183
           YA D+G+ VE SAEDATR+D +YL +V+   +EAGA R+   DTVG++TP + Y     +
Sbjct: 122 YAVDNGLLVELSAEDATRSDYNYLKQVFNEGIEAGARRICACDTVGMLTPEKAYEFYGGL 181

Query: 184 VDAVDVPVSVHCHNDFGMAVANSLAAVEAGAEQVHVTVNGIGERAGNASLEQVVMALKAL 243
              + VP+S HCHNDFG+AVAN+L+ +  GA Q HVTVNGIGERAGNASLE+VV++L +L
Sbjct: 182 TQ-LGVPLSAHCHNDFGLAVANTLSGLRGGASQAHVTVNGIGERAGNASLEEVVVSLYSL 240

Query: 244 YDIELDVRTEMLVELSRLVERLTGVVVPPNTPIVGENAFAHESGIHSHGVIKKAETYEPI 303
           Y+ + ++  EML E S+ V R+TG+ + PN  IVGENAFAHESGIHS GVIKKAETYEPI
Sbjct: 241 YNSKTNINIEMLYETSKTVARMTGIYLQPNKAIVGENAFAHESGIHSDGVIKKAETYEPI 300

Query: 304 RPEDVGHRRRIVLGKHAGRHAIKKKLEEMGIEVTEEQLDEIVRRVKELGDKGKRVTEDDL 363
            PE VGH+RR V+GKH G H I+K+++EMG+ V + + ++I +RVK LGD GK VT+ DL
Sbjct: 301 TPELVGHKRRFVMGKHVGSHIIRKRIKEMGLRVDDARFEQIFQRVKALGDMGKCVTDVDL 360

Query: 364 EAIARDVVGEVPESEAAVKLEEIAVMTGNKFTPTASVRVYLDGEEHEAASTGVGSVDAAI 423
           +AIA D +G +  +E  V+LEE+ +++GNK TPTASV++ +DG E   A  GVG VDAAI
Sbjct: 361 QAIAEDALGVL--AEKPVELEELTIVSGNKVTPTASVKINVDGSEKVEAGVGVGPVDAAI 418

Query: 424 RALREAIEELGMDVELKEYRLEAITGGTDALAEVTVRLEDEDGNVTTARGAAEDIVMASV 483
            A++++I ++  D++L+EY ++AITGGTDAL +V V+L++ D N+ TAR    DI+MASV
Sbjct: 419 VAIKKSIMDVA-DIQLEEYHVDAITGGTDALIDVVVKLKNGD-NIVTARSTQPDIIMASV 476

Query: 484 KAFVRGVNRL 493
           +A + GVN++
Sbjct: 477 EAVLGGVNKI 486


Lambda     K      H
   0.315    0.133    0.364 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 665
Number of extensions: 29
Number of successful extensions: 7
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 499
Length of database: 501
Length adjustment: 34
Effective length of query: 465
Effective length of database: 467
Effective search space:   217155
Effective search space used:   217155
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.5 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis