Align dihydroxy-acid dehydratase subunit (EC 4.2.1.9) (characterized)
to candidate WP_013684023.1 ARCVE_RS06770 dihydroxy-acid dehydratase
Query= metacyc::MONOMER-11919 (549 letters) >NCBI__GCF_000194625.1:WP_013684023.1 Length = 547 Score = 595 bits (1535), Expect = e-174 Identities = 294/547 (53%), Positives = 397/547 (72%), Gaps = 2/547 (0%) Query: 1 MKSDTIKRGIQRAPHRSLLARCGLTDDDFEKPFIGIANSYTDIVPGHIHLRELAEAVKEG 60 M+SD IKRGI+R HR+LL GLTD+D +KPFIG+AN+Y +IVPGH+ L +++AVKEG Sbjct: 1 MRSDEIKRGIERTAHRALLKATGLTDEDMDKPFIGVANAYNNIVPGHMLLDRISDAVKEG 60 Query: 61 VNAAGGVAFEFNTMAICDGIAMNHDGMKYSLASREIVADTVESMAMAHALDGLVLLPTCD 120 + +AGGVAFEF + ICDGIAM HDGM+++L SRE++AD++E+M AH DGLV++ +CD Sbjct: 61 IASAGGVAFEFGVIGICDGIAMGHDGMRFALPSRELIADSIEAMVQAHCFDGLVVVGSCD 120 Query: 121 KIVPGMLMAAARLDIPAIVVTGGPMLPGEFKGRKVDLINVYEGVGTVSAGEMSEDELEEL 180 KIVPGMLMA RLDIPAI VTGGPML + V + +E G AG +SE+EL+ Sbjct: 121 KIVPGMLMAMLRLDIPAIAVTGGPMLAERIGEKVVTIKTAFEAAGEYKAGRISEEELKLY 180 Query: 181 ERCACPGPRSCAGLFTANTMACLTEALGMSLPGCATAHAVSSRKRQIARLSGKRIVEMVQ 240 E SC GL+TANTM LTE LG+SLP C+T+ SSRK +IA+ SGKR+VE+V+ Sbjct: 181 EDYCAAYCGSCQGLYTANTMQILTETLGLSLPYCSTSPCGSSRKLRIAKESGKRVVELVK 240 Query: 241 ENLKPTMIMSQEAFENAVMVDLALGGSTNTTLHIPAIAAEIDGLNINLDLFDELSRVIPH 300 +KP+ I+++E+F NA+ +D+ +GGSTNT LH+PAIA E G+ ++LD+FDE+SR PH Sbjct: 241 RGIKPSDIITEESFRNAITMDMLIGGSTNTVLHLPAIAREA-GIELSLDVFDEISRKTPH 299 Query: 301 IASISPAGEHMMLDLDRAGGIPAVLKTLEDHINRECVTCTGRTVQENIENVKVGHRDVIR 360 I S+ PA E+ + DLD +GG+P +++ + + + E +T +G + + V RD+IR Sbjct: 300 IVSLDPASEYTIADLDESGGVPMLIQRAKKYFSNE-LTISGLRIYDIATKAFVRGRDIIR 358 Query: 361 PLDSPVHSEGGLAILRGNLAPRGSVVKQGAVAEDMMVHEGPAKVFNSEDECMEAIFGGRI 420 + +P+H EGG+AIL GNLA +G VVK AV EDM+ EG AKVF+SE E ++AI + Sbjct: 359 EVTNPIHKEGGIAILYGNLAEKGCVVKAAAVKEDMLRFEGEAKVFDSEQEALKAILNSEV 418 Query: 421 DEGDVIVIRYEGPKGGPGMREMLNPTSAIAGMGLERVALITDGRFSGGTRGPCVGHVSPE 480 ++G+V+VIRY GPKG PGM EML PT+AIAGMGL RVALITDGRFSG TRGPC+GH+SPE Sbjct: 419 EDGNVVVIRYMGPKGAPGMPEMLLPTAAIAGMGLSRVALITDGRFSGATRGPCIGHISPE 478 Query: 481 AMEDGPLAAVNDGDIIRIDIPSRKLEVDLSPREIEERLQSAVKPRRSVKGWLARYRKLAG 540 A G +A V DGD+I IDIP+R++E+ + +EER + + + G+LARY KL Sbjct: 479 AAVGGNIALVEDGDVISIDIPARRIELKVDNNVLEERRKKWKPKEKKLTGYLARYAKLVS 538 Query: 541 SADTGAV 547 A+ GAV Sbjct: 539 GAEKGAV 545 Lambda K H 0.319 0.136 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 878 Number of extensions: 28 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 549 Length of database: 547 Length adjustment: 36 Effective length of query: 513 Effective length of database: 511 Effective search space: 262143 Effective search space used: 262143 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 53 (25.0 bits)
Align candidate WP_013684023.1 ARCVE_RS06770 (dihydroxy-acid dehydratase)
to HMM TIGR00110 (ilvD: dihydroxy-acid dehydratase (EC 4.2.1.9))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00110.hmm # target sequence database: /tmp/gapView.16206.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00110 [M=543] Accession: TIGR00110 Description: ilvD: dihydroxy-acid dehydratase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 2.2e-249 814.4 4.5 2.5e-249 814.3 4.5 1.0 1 lcl|NCBI__GCF_000194625.1:WP_013684023.1 ARCVE_RS06770 dihydroxy-acid deh Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_000194625.1:WP_013684023.1 ARCVE_RS06770 dihydroxy-acid dehydratase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 814.3 4.5 2.5e-249 2.5e-249 1 541 [. 14 546 .. 14 547 .] 0.99 Alignments for each domain: == domain 1 score: 814.3 bits; conditional E-value: 2.5e-249 TIGR00110 1 aarallkatGlkdedlekPiiavvnsyteivPghvhlkdlaklvkeeieaaGgvakefntiavsDGiam 69 a+rallkatGl+ded++kP+i+v+n+y++ivPgh+ l+ ++++vke+i +aGgva+ef++i+++DGiam lcl|NCBI__GCF_000194625.1:WP_013684023.1 14 AHRALLKATGLTDEDMDKPFIGVANAYNNIVPGHMLLDRISDAVKEGIASAGGVAFEFGVIGICDGIAM 82 69******************************************************************* PP TIGR00110 70 gheGmkysLpsreiiaDsvetvvkahalDalvvissCDkivPGmlmaalrlniPaivvsGGpmeagktk 138 gh+Gm+++Lpsre+iaDs+e++v+ah++D+lvv++sCDkivPGmlma+lrl+iPai+v+GGpm a+++ lcl|NCBI__GCF_000194625.1:WP_013684023.1 83 GHDGMRFALPSRELIADSIEAMVQAHCFDGLVVVGSCDKIVPGMLMAMLRLDIPAIAVTGGPMLAERIG 151 ********************************************************************9 PP TIGR00110 139 lsekidlvdvfeavgeyaagklseeeleeiersacPtagsCsGlftansmacltealGlslPgsstlla 207 + ++++ +fea+gey+ag++seeel+ +e++++ ++gsC+Gl+tan+m++lte+lGlslP++st+++ lcl|NCBI__GCF_000194625.1:WP_013684023.1 152 -EKVVTIKTAFEAAGEYKAGRISEEELKLYEDYCAAYCGSCQGLYTANTMQILTETLGLSLPYCSTSPC 219 .6899999************************************************************* PP TIGR00110 208 tsaekkelakksgkrivelvkknikPrdiltkeafenaitldlalGGstntvLhllaiakeagvklsld 276 s++k+++ak+sgkr+velvk+ ikP+di+t+e+f nait+d+++GGstntvLhl+aia+eag++lsld lcl|NCBI__GCF_000194625.1:WP_013684023.1 220 GSSRKLRIAKESGKRVVELVKRGIKPSDIITEESFRNAITMDMLIGGSTNTVLHLPAIAREAGIELSLD 288 ********************************************************************* PP TIGR00110 277 dfdrlsrkvPllaklkPsgkkviedlhraGGvsavlkeldkegllhkdaltvtGktlaetlekvkvlrv 345 fd++srk+P++++l+P++++ i+dl+++GGv+++++ + ++++ ++ lt++G +++++ k+ v+ lcl|NCBI__GCF_000194625.1:WP_013684023.1 289 VFDEISRKTPHIVSLDPASEYTIADLDESGGVPMLIQRA--KKYFSNE-LTISGLRIYDIATKAFVR-- 352 ************************************999..7778777.9*************9999.. PP TIGR00110 346 dqdvirsldnpvkkegglavLkGnlaeeGavvkiagveedilkfeGpakvfeseeealeailggkvkeG 414 ++d+ir++ np++kegg+a+L+Gnlae+G+vvk+a+v+ed+l+feG+akvf+se+eal+ail+ +v++G lcl|NCBI__GCF_000194625.1:WP_013684023.1 353 GRDIIREVTNPIHKEGGIAILYGNLAEKGCVVKAAAVKEDMLRFEGEAKVFDSEQEALKAILNSEVEDG 421 ********************************************************************* PP TIGR00110 415 dvvviryeGPkGgPGmremLaPtsalvglGLgkkvaLitDGrfsGgtrGlsiGhvsPeaaegGaialve 483 +vvviry+GPkG+PGm+emL Pt+a++g+GL + vaLitDGrfsG+trG++iGh+sPeaa+gG+ialve lcl|NCBI__GCF_000194625.1:WP_013684023.1 422 NVVVIRYMGPKGAPGMPEMLLPTAAIAGMGLSR-VALITDGRFSGATRGPCIGHISPEAAVGGNIALVE 489 ********************************9.*********************************** PP TIGR00110 484 dGDkikiDienrkldlevseeelaerrakakkkearevkgaLakyaklvssadkGavl 541 dGD i+iDi++r+++l+v+++ l+err+k+k+ke ++ g+La+yaklvs+a+kGav lcl|NCBI__GCF_000194625.1:WP_013684023.1 490 DGDVISIDIPARRIELKVDNNVLEERRKKWKPKE-KKLTGYLARYAKLVSGAEKGAVF 546 ********************************99.789******************96 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (543 nodes) Target sequences: 1 (547 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.02u 0.00s 00:00:00.02 Elapsed: 00:00:00.02 # Mc/sec: 12.93 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory