Align Argininosuccinate synthase; Citrulline--aspartate ligase; EC 6.3.4.5 (characterized)
to candidate WP_013707412.1 DESAC_RS12360 argininosuccinate synthase
Query= SwissProt::P59846 (400 letters) >NCBI__GCF_000195295.1:WP_013707412.1 Length = 400 Score = 567 bits (1460), Expect = e-166 Identities = 267/390 (68%), Positives = 322/390 (82%) Query: 2 KIVLAYSGGLDTSIILKWLKETYRAEVIAFTADIGQGEEVEEAREKALRTGASKAIALDL 61 KIVLAYSGGLDTS+ILKWL ETY+ V+A+ AD+GQG+E+ E KA TGA++ LDL Sbjct: 6 KIVLAYSGGLDTSVILKWLMETYQCPVVAYAADLGQGDELSEVAVKAKATGAAEVHILDL 65 Query: 62 KEEFVRDFVFPMMRAGAVYEGYYLLGTSIARPLIAKHLVRIAEEEGAEAIAHGATGKGND 121 KEEF RDF+FPM RA A+YEG YLLGTSIARPLIAK V +A++ GA+A++HGATGKGND Sbjct: 66 KEEFARDFIFPMFRANALYEGAYLLGTSIARPLIAKAQVEVAKKTGADAVSHGATGKGND 125 Query: 122 QVRFELTAYALKPDIKVIAPWREWSFQGRKEMIAYAEAHGIPVPVTQEKPYSMDANLLHI 181 QVRFELT AL P++K+IAPWREW QGR + +AYAE HGIPVPVTQEKPYS D NLLHI Sbjct: 126 QVRFELTYMALAPELKIIAPWREWGLQGRADCVAYAEKHGIPVPVTQEKPYSSDRNLLHI 185 Query: 182 SYEGGVLEDPWAEPPKGMFRMTQDPEEAPDAPEYVEVEFFEGDPVAVNGERLSPAALLQR 241 S+EGG+LEDPWAEPP MF +TQ P+EAP+ P+Y+E++F GDPVAV+ +++SP ++ Sbjct: 186 SFEGGILEDPWAEPPADMFVLTQSPQEAPNTPQYLEIDFASGDPVAVDDKKMSPFQIIDH 245 Query: 242 LNEIGGRHGVGRVDIVENRFVGMKSRGVYETPGGTILYHARRAVESLTLDREVLHQRDML 301 LN++GG HG+GRVD+VENR+VG+KSRGVYETPGGTIL A AVESLTLDREV+H RD L Sbjct: 246 LNKVGGEHGIGRVDLVENRYVGLKSRGVYETPGGTILRAAHHAVESLTLDREVMHLRDSL 305 Query: 302 SPKYAELVYYGFWYAPEREALQAYFDHVARSVTGVARLKLYKGNVYVVGRKAPKSLYRQD 361 P+YAELVYYG+W+APER ALQA D + VTG ARL+LYKGNV VVGRKAPKSLYR D Sbjct: 306 IPRYAELVYYGYWFAPERTALQALIDEAQKPVTGTARLQLYKGNVTVVGRKAPKSLYRPD 365 Query: 362 LVSFDEAGGYDQKDAEGFIKIQALRLRVRA 391 LV+F+ G Y Q DA GFI++ ALRL++RA Sbjct: 366 LVTFEAGGDYQQADATGFIRLNALRLKIRA 395 Lambda K H 0.319 0.137 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 554 Number of extensions: 11 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 400 Length of database: 400 Length adjustment: 31 Effective length of query: 369 Effective length of database: 369 Effective search space: 136161 Effective search space used: 136161 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 50 (23.9 bits)
Align candidate WP_013707412.1 DESAC_RS12360 (argininosuccinate synthase)
to HMM TIGR00032 (argG: argininosuccinate synthase (EC 6.3.4.5))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00032.hmm # target sequence database: /tmp/gapView.1128.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00032 [M=394] Accession: TIGR00032 Description: argG: argininosuccinate synthase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 4.8e-153 496.0 0.0 5.7e-153 495.8 0.0 1.0 1 lcl|NCBI__GCF_000195295.1:WP_013707412.1 DESAC_RS12360 argininosuccinate Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_000195295.1:WP_013707412.1 DESAC_RS12360 argininosuccinate synthase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 495.8 0.0 5.7e-153 5.7e-153 1 389 [. 6 394 .. 6 398 .. 0.99 Alignments for each domain: == domain 1 score: 495.8 bits; conditional E-value: 5.7e-153 TIGR00032 1 kvvlaysGGlDtsvalklleek.gaeviavtvdvGqpeedldaieekalklGaekayviDareefvkdy 68 k+vlaysGGlDtsv+lk+l+e + v+a+++d+Gq+ ++l +++ ka++ Ga + ++D +eef++d+ lcl|NCBI__GCF_000195295.1:WP_013707412.1 6 KIVLAYSGGLDTSVILKWLMETyQCPVVAYAADLGQG-DELSEVAVKAKATGAAEVHILDLKEEFARDF 73 89********************889***********9.******************************* PP TIGR00032 69 lfaaiqanavyegkYllstaLaRpliakklvevakkegaeavahGctgKGnDqvRFelsilllnpdlkv 137 +f+ +ana+yeg Yll+t++aRpliak++vevakk ga+av+hG+tgKGnDqvRFel+ ++l+p+lk+ lcl|NCBI__GCF_000195295.1:WP_013707412.1 74 IFPMFRANALYEGAYLLGTSIARPLIAKAQVEVAKKTGADAVSHGATGKGNDQVRFELTYMALAPELKI 142 ********************************************************************* PP TIGR00032 138 iaPvreleli.ReeeieyaaekGievpvekekaysiDenllgrsiEageLEdpsteppediyelvkdpi 205 iaP+re+ l R++ ++ya+++Gi+vpv++ek+ys D+nll++s E+g+LEdp++epp d++ l+++p+ lcl|NCBI__GCF_000195295.1:WP_013707412.1 143 IAPWREWGLQgRADCVAYAEKHGIPVPVTQEKPYSSDRNLLHISFEGGILEDPWAEPPADMFVLTQSPQ 211 *********99********************************************************99 PP TIGR00032 206 ektedepevveieFekGvPvalngeslepvelilkaneiagkhGvGriDivEdRiiglKsReiYEapal 274 e++ ++p+++ei+F +G Pva+++++++p+++i ++n+++g+hG+Gr+D vE+R++glKsR++YE+p+ lcl|NCBI__GCF_000195295.1:WP_013707412.1 212 EAP-NTPQYLEIDFASGDPVAVDDKKMSPFQIIDHLNKVGGEHGIGRVDLVENRYVGLKSRGVYETPGG 279 999.9**************************************************************** PP TIGR00032 275 llLikAhkdLetlvltkdvlefkeiveekyseliYkGlwfdplaealdalikktqervtGtvrvklfkG 343 ++L+ Ah+++e+l+l+++v+++++ +y+el+Y+G+wf p++ al+ali++ q+ vtGt r++l+kG lcl|NCBI__GCF_000195295.1:WP_013707412.1 280 TILRAAHHAVESLTLDREVMHLRDSLIPRYAELVYYGYWFAPERTALQALIDEAQKPVTGTARLQLYKG 348 ********************************************************************* PP TIGR00032 344 naivigrkseyslYdeelvsfekdkefdqkdaiGfikirglqikvy 389 n++v+grk ++slY ++lv+fe ++q da+Gfi++++l+ k+ lcl|NCBI__GCF_000195295.1:WP_013707412.1 349 NVTVVGRKAPKSLYRPDLVTFEAGGDYQQADATGFIRLNALRLKIR 394 *****************************************98865 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (394 nodes) Target sequences: 1 (400 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01 # Mc/sec: 9.59 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory