Align ATP phosphoribosyltransferase (EC 2.4.2.17) (characterized)
to candidate WP_013817408.1 METME_RS03480 ATP phosphoribosyltransferase
Query= reanno::psRCH2:GFF3236 (210 letters) >NCBI__GCF_000214665.1:WP_013817408.1 Length = 208 Score = 295 bits (756), Expect = 3e-85 Identities = 147/199 (73%), Positives = 176/199 (88%) Query: 1 MLTIALSKGRILDDTLPLLAAAGIVPTENPDKSRKLIIPTTQDDVRLLIVRATDVPTYVE 60 MLTIA+SKGRI ++ LP L AGI PT++PDKSRKLI+ TT+ DV+L+I+RATDVPT+VE Sbjct: 1 MLTIAVSKGRIYEEALPFLQQAGITPTDDPDKSRKLILQTTRPDVQLVIIRATDVPTFVE 60 Query: 61 HGAADLGVAGKDVLMEYGGQGLYEPLDLRIANCKLMTAGKVGAPEPKGRLRVATKFVNVA 120 +GAADLG+AGKDVL+E+G + LYEPLDL IA C+LMTAG V APE KGRLRVATK+V A Sbjct: 61 YGAADLGIAGKDVLLEHGAESLYEPLDLGIAGCRLMTAGPVNAPEVKGRLRVATKYVKTA 120 Query: 121 KRYYAEQGRQVDIIKLYGSMELAPLVGLADKIIDVVDTGNTLRANGLEPQELIAHISSRL 180 +RYYA+ G Q +IIKLYGSMELAPLVGLAD I+D+VDTGNTL+ANGLEP+ELIA+I+SRL Sbjct: 121 QRYYADLGIQAEIIKLYGSMELAPLVGLADCIVDLVDTGNTLKANGLEPRELIANITSRL 180 Query: 181 VVNKASMKMQHARIQALID 199 VVNKA+MKM+H IQALID Sbjct: 181 VVNKAAMKMKHQAIQALID 199 Lambda K H 0.319 0.137 0.383 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 214 Number of extensions: 3 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 210 Length of database: 208 Length adjustment: 21 Effective length of query: 189 Effective length of database: 187 Effective search space: 35343 Effective search space used: 35343 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 45 (21.9 bits)
Align candidate WP_013817408.1 METME_RS03480 (ATP phosphoribosyltransferase)
to HMM TIGR00070 (hisG: ATP phosphoribosyltransferase (EC 2.4.2.17))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00070.hmm # target sequence database: /tmp/gapView.22941.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00070 [M=183] Accession: TIGR00070 Description: hisG: ATP phosphoribosyltransferase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 1.3e-59 187.2 0.0 1.6e-59 187.0 0.0 1.0 1 lcl|NCBI__GCF_000214665.1:WP_013817408.1 METME_RS03480 ATP phosphoribosyl Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_000214665.1:WP_013817408.1 METME_RS03480 ATP phosphoribosyltransferase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 187.0 0.0 1.6e-59 1.6e-59 1 183 [] 2 182 .. 2 182 .. 0.95 Alignments for each domain: == domain 1 score: 187.0 bits; conditional E-value: 1.6e-59 TIGR00070 1 lriAlpKGrleeetlkllekaglklskke..erkliasaedeevevlllrakdiptyvekgaadlGitG 67 l+iA+ KGr+ ee+l +l++ag++ ++ +rkli ++++++v+++++ra+d+pt+ve+gaadlGi G lcl|NCBI__GCF_000214665.1:WP_013817408.1 2 LTIAVSKGRIYEEALPFLQQAGITPTDDPdkSRKLILQTTRPDVQLVIIRATDVPTFVEYGAADLGIAG 70 79*********************988766689************************************* PP TIGR00070 68 kDlleEsead.vvelldlgfgkcklvlAvpees.dvesledlkegk.riATkypnltreylekkgvkve 133 kD+l E++a+ ++e ldlg+ c+l+ A p + +v+ g+ r+ATky++++++y+++ g+++e lcl|NCBI__GCF_000214665.1:WP_013817408.1 71 KDVLLEHGAEsLYEPLDLGIAGCRLMTAGPVNApEVK-------GRlRVATKYVKTAQRYYADLGIQAE 132 ******98887*********************94443.......445********************** PP TIGR00070 134 ivkleGavElapllgladaIvDivetGttLrengLkiieeilessarlia 183 i+kl+G++Elapl+glad+IvD+v tG+tL++ngL+ e i ++++rl++ lcl|NCBI__GCF_000214665.1:WP_013817408.1 133 IIKLYGSMELAPLVGLADCIVDLVDTGNTLKANGLEPRELIANITSRLVV 182 ***********************************************985 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (183 nodes) Target sequences: 1 (208 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.00 # Mc/sec: 8.58 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory