Align glutamine synthetase (EC 6.3.1.2) (characterized)
to candidate WP_013819544.1 METME_RS14745 glutamate--ammonia ligase
Query= BRENDA::P0A1P6 (469 letters) >NCBI__GCF_000214665.1:WP_013819544.1 Length = 469 Score = 703 bits (1815), Expect = 0.0 Identities = 333/469 (71%), Positives = 388/469 (82%) Query: 1 MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFFEEGKMFDGSSIGGWKG 60 MS E+VL M+ E++V+FVD RF DT+GKEQHVT PAH ++ + FEEG MFDGSS+ GWK Sbjct: 1 MSVENVLKMIQENDVQFVDFRFCDTRGKEQHVTFPAHTIDEDTFEEGNMFDGSSVAGWKH 60 Query: 61 INESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYDRDPRSIAKRAEDYLRATG 120 INESDM+LMPD STA IDPFF D T+I+RCDI+EP +QGY RDPRSIAKRAE Y+++TG Sbjct: 61 INESDMILMPDPSTAKIDPFFDDKTVILRCDIIEPKDMQGYGRDPRSIAKRAEAYMQSTG 120 Query: 121 IADTVLFGPEPEFFLFDDIRFGASISGSHVAIDDIEGAWNSSTKYEGGNKGHRPGVKGGY 180 IADT FGPE EFF+FDD+R+ A + G ID E WNS YE GN GHRPG+KGGY Sbjct: 121 IADTAFFGPENEFFIFDDVRWSAEMGGCSYKIDSEEAGWNSEKVYENGNIGHRPGIKGGY 180 Query: 181 FPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAGQNEVATRFNTMTKKADEIQIYK 240 FPVPPVDS QD+RS MCLV+E++G+ E HHHEVATAGQ E+ +FNT+ KKADE+ K Sbjct: 181 FPVPPVDSFQDMRSAMCLVLEELGMTTEVHHHEVATAGQCEIGVKFNTLVKKADEVLELK 240 Query: 241 YVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLAKNGTNLFSGDKYAGLSEQALYYIG 300 YVV N+AH +GKTATFMPKP+ GDNGSGMH H SLAK G NLFSGD Y GLSE ALYYIG Sbjct: 241 YVVANIAHAYGKTATFMPKPLVGDNGSGMHVHQSLAKGGVNLFSGDLYGGLSETALYYIG 300 Query: 301 GVIKHAKAINALANPTTNSYKRLVPGYEAPVMLAYSARNRSASIRIPVVASPKARRIEVR 360 G+IKHAKA+NAL N +TNSYKRLVPG+EAPVMLAYSARNRSASIRIP V +PK RRIEVR Sbjct: 301 GIIKHAKALNALTNASTNSYKRLVPGFEAPVMLAYSARNRSASIRIPYVTNPKGRRIEVR 360 Query: 361 FPDPAANPYLCFAALLMAGLDGIKNKIHPGEAMDKNLYDLPPEEAKEIPQVAGSLEEALN 420 FPD ANPYL F+A+LMAGLDGI+NKIHPGEAMDK+LYDLPPEE K IPQVA SL+ AL+ Sbjct: 361 FPDSTANPYLAFSAMLMAGLDGIQNKIHPGEAMDKDLYDLPPEEEKAIPQVAFSLDSALD 420 Query: 421 ALDLDREFLKAGGVFTDEAIDAYIALRREEDDRVRMTPHPVEFELYYSV 469 ALD DREFL GGVFTD+AID YI L+R++ R+RM+ HPVEF++YYS+ Sbjct: 421 ALDADREFLTRGGVFTDDAIDGYIELKRQDVTRLRMSTHPVEFDMYYSL 469 Lambda K H 0.318 0.136 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 774 Number of extensions: 23 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 469 Length of database: 469 Length adjustment: 33 Effective length of query: 436 Effective length of database: 436 Effective search space: 190096 Effective search space used: 190096 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 51 (24.3 bits)
Align candidate WP_013819544.1 METME_RS14745 (glutamate--ammonia ligase)
to HMM TIGR00653 (glnA: glutamine synthetase, type I (EC 6.3.1.2))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00653.hmm # target sequence database: /tmp/gapView.3651.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00653 [M=462] Accession: TIGR00653 Description: GlnA: glutamine synthetase, type I Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 8.4e-209 679.9 0.0 9.5e-209 679.7 0.0 1.0 1 lcl|NCBI__GCF_000214665.1:WP_013819544.1 METME_RS14745 glutamate--ammonia Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_000214665.1:WP_013819544.1 METME_RS14745 glutamate--ammonia ligase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 679.7 0.0 9.5e-209 9.5e-209 1 462 [] 4 468 .. 4 468 .. 0.99 Alignments for each domain: == domain 1 score: 679.7 bits; conditional E-value: 9.5e-209 TIGR00653 1 eevlkllkeenvkfvdlrfvDikGklkkveipvseleeealeegiaFDgssveGfksieesDlllkpdp 69 e+vlk+++e++v+fvd+rf+D++Gk+++v++p+++++e+++eeg +FDgssv G+k+i+esD++l+pdp lcl|NCBI__GCF_000214665.1:WP_013819544.1 4 ENVLKMIQENDVQFVDFRFCDTRGKEQHVTFPAHTIDEDTFEEGNMFDGSSVAGWKHINESDMILMPDP 72 6799***************************************************************** PP TIGR00653 70 etlvivPfraekvlrvicdvyepvtkepyerdpRsiakraeeelk.tklgdevyfGpEaEFflfdkvef 137 +t++i+Pf ++k+++++cd+ ep +++ y rdpRsiakrae +++ t+++d+++fGpE+EFf+fd+v+ lcl|NCBI__GCF_000214665.1:WP_013819544.1 73 STAKIDPFFDDKTVILRCDIIEPKDMQGYGRDPRSIAKRAEAYMQsTGIADTAFFGPENEFFIFDDVRW 141 ********************************************************************* PP TIGR00653 138 keasnssflevdseegewn..reveegnkgykikkkggYfdvepvDkakdirrelvlaleelglevevs 204 + ++ +++dsee+ wn + +e+gn g+++ kggYf+v+pvD+ +d+r+ ++l+leelg+++ev+ lcl|NCBI__GCF_000214665.1:WP_013819544.1 142 SAEMGGCSYKIDSEEAGWNseKVYENGNIGHRPGIKGGYFPVPPVDSFQDMRSAMCLVLEELGMTTEVH 210 *******************98899********************************************* PP TIGR00653 205 HHEvata.qaEidikfdklvkaaDeivlyKyvvknvakkhGktatFmpKplfgdngsGmHvhlslwkdg 272 HHEvata q Ei++kf++lvk+aDe++ +Kyvv n+a+ +GktatFmpKpl+gdngsGmHvh+sl k g lcl|NCBI__GCF_000214665.1:WP_013819544.1 211 HHEVATAgQCEIGVKFNTLVKKADEVLELKYVVANIAHAYGKTATFMPKPLVGDNGSGMHVHQSLAKGG 279 ********************************************************************* PP TIGR00653 273 enlfageegyagLsetalyyigGilkHakalaAltnptvnsYkRLvpGyEAPvylaysaknRsaliRiP 341 nlf+g+ y gLsetalyyigGi+kHakal+Altn+++nsYkRLvpG+EAPv+laysa+nRsa+iRiP lcl|NCBI__GCF_000214665.1:WP_013819544.1 280 VNLFSGDL-YGGLSETALYYIGGIIKHAKALNALTNASTNSYKRLVPGFEAPVMLAYSARNRSASIRIP 347 ********.************************************************************ PP TIGR00653 342 asanpkakRiEvRspDpsanpYLafaallmAgldGiknkidpgepldknlyelseeelkelGieqlpes 410 +++npk++RiEvR+pD++anpYLaf+a+lmAgldGi+nki+pge++dk+ly+l++ee k i+q++ s lcl|NCBI__GCF_000214665.1:WP_013819544.1 348 YVTNPKGRRIEVRFPDSTANPYLAFSAMLMAGLDGIQNKIHPGEAMDKDLYDLPPEEEKA--IPQVAFS 414 *********************************************************999..******* PP TIGR00653 411 Leealdelesdk..evlkevlgeelieafielkrkEveelrlkvhpvElekyld 462 L+ ald+l++d+ ++ ++v+++++i+ +ielkr++v +lr++ hpvE+ +y++ lcl|NCBI__GCF_000214665.1:WP_013819544.1 415 LDSALDALDADRefLTRGGVFTDDAIDGYIELKRQDVTRLRMSTHPVEFDMYYS 468 ************888999**********************************95 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (462 nodes) Target sequences: 1 (469 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.02u 0.00s 00:00:00.02 Elapsed: 00:00:00.02 # Mc/sec: 8.86 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory