Alignments for a candidate for aspS2 in Methylomonas methanica MC09

GapMind for Amino acid biosynthesis

Alignments for a candidate for aspS2 in Methylomonas methanica MC09

Align Aspartate--tRNA(Asp/Asn) ligase; Aspartyl-tRNA synthetase; AspRS; Non-discriminating aspartyl-tRNA synthetase; ND-AspRS; EC 6.1.1.23 (characterized)
to candidate WP_013820279.1 METME_RS18575 aspartate--tRNA ligase

Query= SwissProt::Q51422
         (591 letters)



>NCBI__GCF_000214665.1:WP_013820279.1
          Length = 596

 Score =  792 bits (2045), Expect = 0.0
 Identities = 389/587 (66%), Positives = 474/587 (80%), Gaps = 2/587 (0%)

Query: 2   MRSHYCGQLNESLDGQEVTLCGWVHRRRDHGGVIFLDVRDREGLAQVVFDPDRAETFAKA 61
           MRSH CG+LN    GQ V LCGWVHRRRDHGGVIF+D+RDR GL QVVFDPD +++FA A
Sbjct: 1   MRSHKCGELNTQHLGQTVALCGWVHRRRDHGGVIFIDLRDRAGLVQVVFDPDLSDSFAIA 60

Query: 62  DRVRSEFVVKITGKVRLRPEGARNPNMASGSIEVLGYELEVLNQAETPPFPLDEYSDVGE 121
           + VRSE+V++I G VR RPEG  N NM +G IEVLG  +EVLN++ETPPFPL+   +V E
Sbjct: 61  ESVRSEYVLRIEGVVRDRPEGTHNANMFTGQIEVLGKHIEVLNESETPPFPLESEIEVNE 120

Query: 122 ETRLRYRFIDLRRPEMAAKLKLRARITSSIRRYLDDNGFLDVETPILGRPTPEGARDYLV 181
           ETRLR+R+IDLRR  M  ++K+R  +T  +R+YLDDN F ++ETP L + TPEGARDY+V
Sbjct: 121 ETRLRFRYIDLRRTVMQQRMKVRRDVTRHLRQYLDDNEFFEIETPYLTKATPEGARDYIV 180

Query: 182 PSRTYPGHFFALPQSPQLFKQLLMVAGFDRYYQIAKCFRDEDLRADRQPEFTQIDIETSF 241
           PSRT+P  FFALPQSPQL+KQLLMVAG +RYYQ+ +CFRDEDLRADRQPEFTQ+DIETSF
Sbjct: 181 PSRTHPNAFFALPQSPQLYKQLLMVAGMERYYQVVRCFRDEDLRADRQPEFTQLDIETSF 240

Query: 242 LDESDIIGITEKMVRQLFKEVLDVEF-DEFPHMPFEEAMRRYGSDKPDLRIPLELVDVAD 300
           ++E  I+ I E+M+R+LFK++++V+  D FP M   EAMRRYGSD+PDLRIPLELVD+AD
Sbjct: 241 MNEQQIMAIMEEMIRRLFKDIINVDLGDPFPVMSHAEAMRRYGSDRPDLRIPLELVDIAD 300

Query: 301 QLKEVEFKVFSGPANDPKGRVAALRVPGAASMPRSQIDDYTKFVGIYGAKGLAYIKVNER 360
           +++EV+FKVFSGPANDP GRV A+RVP    M RS ID+ +KFVGIYGAKGLAYIKVN+ 
Sbjct: 301 EMREVDFKVFSGPANDPHGRVVAMRVPEGGEMSRSVIDELSKFVGIYGAKGLAYIKVNDL 360

Query: 361 AKGVEGLQSPIVKFIPEANLNVILDRVGAVDGDIVFFGADKAKIVCDALGALRIKVGHDL 420
           A G EGLQSPIVKF P+     ++ +VGA +GD+VFFGADKA IV +A+GALR+K+G D 
Sbjct: 361 AAGAEGLQSPIVKFAPDEVWAKVMAKVGAQNGDLVFFGADKADIVNEAMGALRVKLGLDR 420

Query: 421 KLLTREWAPMWVVDFPMFEENDDGS-LSALHHPFTSPKCTPAELEANPGAALSRAYDMVL 479
            LLT +W P+WVVDFPMF  +D     +A+HHPFT+P C+  EL+A+PG ALSRAYD+VL
Sbjct: 421 NLLTGDWKPLWVVDFPMFAWDDKAQRYTAIHHPFTAPSCSVEELKADPGHALSRAYDLVL 480

Query: 480 NGTELGGGSIRIHDKSMQQAVFRVLGIDEAEQEEKFGFLLDALKYGAPPHGGLAFGLDRL 539
           NGTE+GGGSIRI+   MQQ VF +LGI   E  EKFGFLLDALKYGAPPHGG+AFGLDRL
Sbjct: 481 NGTEVGGGSIRINRTEMQQIVFAILGIGHEEAREKFGFLLDALKYGAPPHGGIAFGLDRL 540

Query: 540 VMLMTGASSIREVIAFPKTQSAGDVMTQAPGSVDGKALRELHIRLRE 586
           VMLMTGASSIR+VIAFPKTQ+A   +  AP  V  + L+EL IRLR+
Sbjct: 541 VMLMTGASSIRDVIAFPKTQTAACPLFNAPALVSDEQLKELGIRLRK 587


Lambda     K      H
   0.321    0.140    0.411 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1143
Number of extensions: 44
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 591
Length of database: 596
Length adjustment: 37
Effective length of query: 554
Effective length of database: 559
Effective search space:   309686
Effective search space used:   309686
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis