Alignments for a candidate for prs in Desulfotomaculum ruminis DSM 2154

GapMind for Amino acid biosynthesis

Alignments for a candidate for prs in Desulfotomaculum ruminis DSM 2154

Align Ribose-phosphate pyrophosphokinase; RPPK; 5-phospho-D-ribosyl alpha-1-diphosphate synthase; Phosphoribosyl diphosphate synthase; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PPRibP synthase; PRPP synthase; PRPPase; EC 2.7.6.1 (characterized)
to candidate WP_013840250.1 DESRU_RS00870 ribose-phosphate pyrophosphokinase

Query= SwissProt::P14193
         (317 letters)



>NCBI__GCF_000215085.1:WP_013840250.1
          Length = 315

 Score =  421 bits (1082), Expect = e-122
 Identities = 207/312 (66%), Positives = 251/312 (80%)

Query: 6   GDKNLKIFSLNSNPELAKEIADIVGVQLGKCSVTRFSDGEVQINIEESIRGCDCYIIQST 65
           G K LKIF+ N+NP+LA+EI   +GV +G   V+RFSDGE++  +EES+RG D +IIQ T
Sbjct: 4   GSKRLKIFAGNANPDLAREICQYLGVAMGASKVSRFSDGEIRAEVEESVRGADAFIIQPT 63

Query: 66  SDPVNEHIMELLIMVDALKRASAKTINIVIPYYGYARQDRKARSREPITAKLFANLLETA 125
           S PVNEH+MELLIMVDAL+RASA+ I  V+PYYGYARQDRK R+R PITAKL AN++  +
Sbjct: 64  STPVNEHLMELLIMVDALRRASARRITAVVPYYGYARQDRKTRARAPITAKLVANIIIAS 123

Query: 126 GATRVIALDLHAPQIQGFFDIPIDHLMGVPILGEYFEGKNLEDIVIVSPDHGGVTRARKL 185
           G  R+I +DLHA QIQGFFDIP+DHL GVPIL EYF     +D+V+VSPD GGVTRAR L
Sbjct: 124 GCRRMITMDLHAGQIQGFFDIPVDHLPGVPILAEYFHQNLKDDVVVVSPDIGGVTRARDL 183

Query: 186 ADRLKAPIAIIDKRRPRPNVAEVMNIVGNIEGKTAILIDDIIDTAGTITLAANALVENGA 245
           A+R+ AP+AIIDKRRP PNVAEV N++G+I+GKT I+IDDIIDTAGTIT  A AL+E GA
Sbjct: 184 AERIGAPLAIIDKRRPEPNVAEVTNVIGSIKGKTVIMIDDIIDTAGTITKGAAALMERGA 243

Query: 246 KEVYACCTHPVLSGPAVERINNSTIKELVVTNSIKLPEEKKIERFKQLSVGPLLAEAIIR 305
           KE+  CCTH VLSGPA++R+  S IKE+VVTN+I LP EK I++ K LSV PLL EAIIR
Sbjct: 244 KEIRVCCTHAVLSGPAIQRLQESVIKEVVVTNTIPLPPEKMIDKIKVLSVAPLLGEAIIR 303

Query: 306 VHEQQSVSYLFS 317
           +HE  SVS LFS
Sbjct: 304 IHEDLSVSKLFS 315


Lambda     K      H
   0.319    0.137    0.383 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 301
Number of extensions: 8
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 317
Length of database: 315
Length adjustment: 27
Effective length of query: 290
Effective length of database: 288
Effective search space:    83520
Effective search space used:    83520
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 48 (23.1 bits)

Align candidate WP_013840250.1 DESRU_RS00870 (ribose-phosphate pyrophosphokinase)
to HMM TIGR01251 (prs: ribose-phosphate diphosphokinase (EC 2.7.6.1))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR01251.hmm
# target sequence database:        /tmp/gapView.8353.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01251  [M=309]
Accession:   TIGR01251
Description: ribP_PPkin: ribose-phosphate diphosphokinase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
   3.3e-128  412.9   0.3   3.7e-128  412.7   0.3    1.0  1  lcl|NCBI__GCF_000215085.1:WP_013840250.1  DESRU_RS00870 ribose-phosphate p


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000215085.1:WP_013840250.1  DESRU_RS00870 ribose-phosphate pyrophosphokinase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  412.7   0.3  3.7e-128  3.7e-128       2     309 .]       9     315 .]       8     315 .] 0.99

  Alignments for each domain:
  == domain 1  score: 412.7 bits;  conditional E-value: 3.7e-128
                                 TIGR01251   2 kilsgssskelaekvaknlglelgdvevkkFadgElyvrieesvrgkdvfiivqstsapvndalmelll 70 
                                               ki++g+++++la++++++lg+ +g ++v +F+dgE+  ++eesvrg d fii q ts+pvn++lmell+
  lcl|NCBI__GCF_000215085.1:WP_013840250.1   9 KIFAGNANPDLAREICQYLGVAMGASKVSRFSDGEIRAEVEESVRGADAFII-QPTSTPVNEHLMELLI 76 
                                               89**************************************************.**************** PP

                                 TIGR01251  71 lidalkrasaksvtaviPyygYaRqdkkaksrepisaklvaklleeaGadrvltvdlHseqiqgfFdvp 139
                                               ++dal+rasa+++tav+PyygYaRqd+k++ r pi+aklva+++ + G +r++t+dlH+ qiqgfFd+p
  lcl|NCBI__GCF_000215085.1:WP_013840250.1  77 MVDALRRASARRITAVVPYYGYARQDRKTRARAPITAKLVANIIIASGCRRMITMDLHAGQIQGFFDIP 145
                                               ********************************************************************* PP

                                 TIGR01251 140 venlsaspklieelkkkelknlvvvsPDkGaverakkvakklglelaiieKeRdskenevevtnllgdv 208
                                               v++l + p l+e+++++ ++++vvvsPD G+v+ra+++a+++g++laii+K+R+ + n++evtn++g +
  lcl|NCBI__GCF_000215085.1:WP_013840250.1 146 VDHLPGVPILAEYFHQNLKDDVVVVSPDIGGVTRARDLAERIGAPLAIIDKRRP-EPNVAEVTNVIGSI 213
                                               ******************************************************.888*********** PP

                                 TIGR01251 209 egkdvvivDDiisTggTlvkaaelLkekGAkkvivaathgvfsgdAlerlaeagveevivtntilv.ee 276
                                               +gk+v+++DDii+T+gT++k+a +L e+GAk++ v++th+v+sg+A++rl+e++++ev+vtnti+   e
  lcl|NCBI__GCF_000215085.1:WP_013840250.1 214 KGKTVIMIDDIIDTAGTITKGAAALMERGAKEIRVCCTHAVLSGPAIQRLQESVIKEVVVTNTIPLpPE 282
                                               ******************************************************************999 PP

                                 TIGR01251 277 kklpkvseisvapliaeaiarihenesvsslfd 309
                                               k ++k++++svapl+ eai+rihe+ svs+lf+
  lcl|NCBI__GCF_000215085.1:WP_013840250.1 283 KMIDKIKVLSVAPLLGEAIIRIHEDLSVSKLFS 315
                                               *******************************95 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (309 nodes)
Target sequences:                          1  (315 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.00u 0.00s 00:00:00.00 Elapsed: 00:00:00.00
# Mc/sec: 11.03
//
[ok]

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis