Alignments for a candidate for asd-S-transferase in Desulfotomaculum ruminis DSM 2154

GapMind for Amino acid biosynthesis

Alignments for a candidate for asd-S-transferase in Desulfotomaculum ruminis DSM 2154

Align Homocysteine formation from aspartate semialdehyde (DUF39 component) (characterized)
to candidate WP_013840252.1 DESRU_RS00885 hypothetical protein

Query= reanno::Miya:8500721
         (390 letters)



>NCBI__GCF_000215085.1:WP_013840252.1
          Length = 399

 Score =  434 bits (1117), Expect = e-126
 Identities = 217/384 (56%), Positives = 271/384 (70%), Gaps = 2/384 (0%)

Query: 6   VNKTIAEINERIRQGKAVVLNAEEMTEAVRRMGKEKAAREIDVVTTGTFSPMCSSGLLFN 65
           V +T AEIN RIR GKAVVL AEE+   VR  G  K A E+DVVTTGTF PMCSSG   N
Sbjct: 3   VERTYAEINARIRAGKAVVLTAEEVIGKVREQGLAKTAAEVDVVTTGTFGPMCSSGAFLN 62

Query: 66  IGQQDPPTLKTAKVWMNDVPAYAGLAAVDSYLGATEPTEDDPLNKVYPGRFKYGGGHVIE 125
            G   P  ++  KV +N+VPAY G+AAVD+Y+G TE  EDDP N  +PG F+YGGGHVIE
Sbjct: 63  FGHSSP-RMRMQKVSLNNVPAYTGIAAVDAYIGVTEIPEDDPANNNFPGEFRYGGGHVIE 121

Query: 126 DLVRGKAVHLRAEAYGTDCYPRKSLDKKITLSELPYAHLLNPRNCYQNYNAAVNLTSRII 185
           DLV  K V L+A +YGTDCYPRK ++  ITL ++  A L NPRN YQNYN AVNL+ + I
Sbjct: 122 DLVSHKPVKLKALSYGTDCYPRKEIETTITLDDMNEAILFNPRNAYQNYNCAVNLSEKTI 181

Query: 186 YTYMGPLKPNLRNVNFATAGRISPLFNDPLFRTIGLGTRIFLGGGTGYVLGAGTQHVAA- 244
           YTYMG LKP L N  ++T+G++SPL  DP F TIG+GTRIFLGGG GYV+  GTQH    
Sbjct: 182 YTYMGTLKPRLGNATYSTSGQLSPLMKDPNFATIGIGTRIFLGGGIGYVVWNGTQHFPGW 241

Query: 245 PKRTERGLPLSPAGTLMLKGDLKGMNARYLRGLSFLGYGCSLAVGVGIPIPILNEEIAWF 304
            K  E  +  S  GTL + GDLK M  ++LRG S+ GYG +L VG+GIPIPILNEEI  F
Sbjct: 242 LKDAEGNIVGSAGGTLSVLGDLKQMKPQWLRGTSYQGYGATLTVGLGIPIPILNEEILRF 301

Query: 305 TGVDDSDIQMPVKDYGHDYPNCLPRVIQHVTYEDLKSGEVEIMGKKVETVPMTSYPLSLE 364
             + D ++  PV DY  +YPN +   +  V+Y  LKSG++ + G+++ TVP++SYP + E
Sbjct: 302 AALSDEELHAPVVDYSDNYPNRVGGNLGLVSYAQLKSGKITVQGQEIPTVPLSSYPKARE 361

Query: 365 VANTLKSWIEKGEFLLTEPVELLP 388
           +A TLK WI+KG+FLL EPV+LLP
Sbjct: 362 IARTLKEWIQKGDFLLGEPVQLLP 385


Lambda     K      H
   0.318    0.137    0.409 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 533
Number of extensions: 26
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 390
Length of database: 399
Length adjustment: 31
Effective length of query: 359
Effective length of database: 368
Effective search space:   132112
Effective search space used:   132112
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis