Align aspartate kinase / homoserine dehydrogenase (EC 2.7.2.4; EC 1.1.1.3) (characterized)
to candidate WP_014026853.1 PYRFU_RS06440 aspartate kinase
Query= reanno::Cola:Echvi_2000 (815 letters) >NCBI__GCF_000223395.1:WP_014026853.1 Length = 480 Score = 205 bits (521), Expect = 5e-57 Identities = 139/459 (30%), Positives = 238/459 (51%), Gaps = 18/459 (3%) Query: 3 IIKFGGSSIANYENIQKVFSIIEQKSEKEAFALVFSAFGGVTEQLLQCANIAQQSEESYH 62 + KFGGS + + E+ + I E+ + + LV SA GVT++LL+ +A+ E S Sbjct: 19 VAKFGGSVLKSGEDYARAAEITEELQREHSVILVVSAMKGVTDRLLR---LARDFEASPS 75 Query: 63 TILQELEKRHLEIVKKL-VPVQQQSTALTFVKVRFNELGDLFHGIYLIKECSNRTMDYVL 121 + EL + H+E ++ V + A + + +EL + + ++ E + + +DYV+ Sbjct: 76 VEIPELYQMHVEALRGAGVSGRLFGEAFSRIARLIDELTKIIWALRVLGEVTPKALDYVV 135 Query: 122 SFGERLSNFILAAGLQAKGIGTSYVDARDL-VKTDDRFGHAKVNFKTTNKLIQDHFKSHD 180 SFGERLS I+ A L+++GI + + + TDDRFG A + T ++ Sbjct: 136 SFGERLSAVIMEAVLRSRGIEAKALTGWEAGIVTDDRFGEANPIMEETVPRVRSRLLPLT 195 Query: 181 D---IKVITGFIGSTEKGETTTVGRSGSDYTASIFAAALGAEQVEIWTDVSGVMTADPRL 237 + + ++TGFI +T KG+ TT+GR GSDYTAS+ A+ LGA +V +TDV GVMTADPRL Sbjct: 196 EKGIVPIVTGFIAATRKGDITTLGRGGSDYTASLIASILGATEVHFYTDVPGVMTADPRL 255 Query: 238 VYTAFTIPQLSYNEAMELSHFGAKVVFPATMQPAMKEDIPIYIKNTFKPDEAGTRISKDS 297 + A TIP+L EA+ELS G K P T +P + I I + P T + + Sbjct: 256 IPEARTIPRLCVIEALELSRVGGKKFHPRTFEPLLYSKIRARILDAKDPHGPHTIVEPEC 315 Query: 298 GEGKIIKGISSMDNISILNVQGPGLVEVVGVSQRFFGTLANNGINIILISQASSEHSICV 357 + +K ++ M ++++ ++G + +G + G+NI+ ISQ +E I + Sbjct: 316 ID--TLKAVAVMRGLAVVRLEGAVMAGRIGTAMMVTSVSKRAGVNIVAISQPVTETRIEI 373 Query: 358 AIASKDASRAKSVIEEEFRYEIQSGEMD-EIQIVPDMAVIAVVGENMQHNPGASGRMF-Q 415 +A +DA R + EE R + E+D + + ++ + +VG ++ NP R+ + Sbjct: 374 VVAEEDAERLAKALSEEIRNQ----ELDVNVMVETGLSAVTIVGYGLR-NPSLRARVLSE 428 Query: 416 ALG-RNNVNVAAIAQGSSELNISAVITQADLQKALNALH 453 AL + +I G + +++ V D + +H Sbjct: 429 ALSLEPEPELRSITTGFHDASLTIVTNARDAVRIAERIH 467 Score = 34.3 bits (77), Expect = 2e-05 Identities = 29/140 (20%), Positives = 61/140 (43%), Gaps = 13/140 (9%) Query: 361 SKDASRAKSVIEEEFRYEIQSGEMDEIQIVPDMAVIAVVGENMQHNPGASGRMFQALGRN 420 +KD +++E E +++ + ++ +AV+ + G M G + + R Sbjct: 301 AKDPHGPHTIVEPECIDTLKA-----VAVMRGLAVVRLEGAVMAGRIGTAMMVTSVSKRA 355 Query: 421 NVNVAAIAQGSSELNISAVITQADLQKALNALHEAF--------FLSDYKVLHLFLVGVG 472 VN+ AI+Q +E I V+ + D ++ AL E + + + + +VG G Sbjct: 356 GVNIVAISQPVTETRIEIVVAEEDAERLAKALSEEIRNQELDVNVMVETGLSAVTIVGYG 415 Query: 473 LIGKALTKMIHQQLKNLQEE 492 L +L + + +L+ E Sbjct: 416 LRNPSLRARVLSEALSLEPE 435 Lambda K H 0.317 0.134 0.375 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 739 Number of extensions: 36 Number of successful extensions: 5 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 2 Number of HSP's successfully gapped: 2 Length of query: 815 Length of database: 480 Length adjustment: 37 Effective length of query: 778 Effective length of database: 443 Effective search space: 344654 Effective search space used: 344654 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 54 (25.4 bits)
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory