Alignments for a candidate for argJ in Methylomicrobium alcaliphilum 20Z

GapMind for Amino acid biosynthesis

Alignments for a candidate for argJ in Methylomicrobium alcaliphilum 20Z

Align glutamate N-acetyltransferase/amino-acid acetyltransferase; EC 2.3.1.35 2.3.1.1 (characterized)
to candidate WP_014146969.1 MEALZ_RS02265 bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ

Query= CharProtDB::CH_000559
         (406 letters)



>NCBI__GCF_000968535.2:WP_014146969.1
          Length = 404

 Score =  371 bits (952), Expect = e-107
 Identities = 206/395 (52%), Positives = 263/395 (66%), Gaps = 8/395 (2%)

Query: 16  IDGIALYTAQAGVKKPGHTDLTLIAVAAGSTVGAVFTTNRFCAAPVHIAKSHLFDEDGVR 75
           +DGI L TA AG+K+    DL LI +  GST  AVFT N FCAAPV +AK HL  +   R
Sbjct: 14  VDGIELGTACAGIKQTQRDDLLLIQMREGSTCAAVFTQNAFCAAPVVVAKEHL--QQAPR 71

Query: 76  ALVINTGNANAGTGAQGRIDALAVCAAAARQIGCKPNQVMPFSTGVILEPLPADKIIAAL 135
            L+IN+GNANAGTG +G  DAL+ CA  A   G    QV+PFSTGVI EPL  DKI AAL
Sbjct: 72  WLLINSGNANAGTGKRGLDDALSTCADLAELAGADARQVLPFSTGVIGEPLAVDKIKAAL 131

Query: 136 PK----MQPAFWNEAARAIMTTDTVPKAASREGKVGDQHTVRATGIAKGSGMIHPNMATM 191
           PK    +    W +AARAIMTTDT PK AS    +G + T+  TGI+KG+GMI PNMATM
Sbjct: 132 PKAFANLAEDHWEQAARAIMTTDTFPKGASFTLGIGGE-TITLTGISKGAGMIQPNMATM 190

Query: 192 LGFIATDAKVSQPVLQLMTQEIADETFNTITVDGDTSTNDSFVIIATGKNSQSEIDNIAD 251
           L FIATDAK+ + +LQ      A+ +FN ITVDGDTSTND+ V++A+G +   E+     
Sbjct: 191 LSFIATDAKIERELLQACLARAAELSFNRITVDGDTSTNDACVLMASGCSKVPELTE-GS 249

Query: 252 PRYAQLKELLCSLALELAQAIVRDGEGATKFITVRVENAKTCDEARQAAYAAARSPLVKT 311
           P Y    + +  +  +L++AIVRDGEGATK + + +  A   +EA + A   A SPLVKT
Sbjct: 250 PAYQVFADAVLKICKQLSEAIVRDGEGATKLMRIVINQALDDEEAVRVAKTIAHSPLVKT 309

Query: 312 AFFASDPNLGKRLAAIGYADVADLDTDLVEMYLDDILVAEHGGRAASYTEAQGQAVMSKD 371
           AFFASDPN G+ LAA+G A V ++D + V+++L D+ +   GGRA  YTEA GQ VM++ 
Sbjct: 310 AFFASDPNWGRILAAVGRAGVDNMDLERVDIHLGDVCIVRGGGRAEDYTEAAGQRVMNEP 369

Query: 372 EITVRIKLHRGQAAATVYTCDLSHGYVSINADYRS 406
           EITV + L RG A+  V TCD S+ YV INA+YR+
Sbjct: 370 EITVTVTLGRGSASQEVLTCDFSYDYVRINAEYRT 404


Lambda     K      H
   0.317    0.130    0.367 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 386
Number of extensions: 14
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 406
Length of database: 404
Length adjustment: 31
Effective length of query: 375
Effective length of database: 373
Effective search space:   139875
Effective search space used:   139875
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis