Align Aspartate--tRNA(Asp/Asn) ligase; EC 6.1.1.23; Aspartyl-tRNA synthetase; AspRS; Non-discriminating aspartyl-tRNA synthetase; ND-AspRS (uncharacterized)
to candidate WP_015797990.1 AFER_RS02725 aspartate--tRNA ligase
Query= curated2:A4X5Y3 (607 letters) >NCBI__GCF_000023265.1:WP_015797990.1 Length = 588 Score = 504 bits (1299), Expect = e-147 Identities = 279/586 (47%), Positives = 371/586 (63%), Gaps = 20/586 (3%) Query: 2 IRTHDAGSLRATDAGSTVTLAGWVARRRDHGG-VIFVDLRDGSGVAQVVLREEDAHVLRN 60 +RTH G+L + V++ GWVAR R+HG ++FVD+RD +G Q V+ + L Sbjct: 12 LRTHYIGALDESLLDRDVSVCGWVARIREHGEHLVFVDVRDHTGTVQCVMDGRGS--LHT 69 Query: 61 EYCVRVTGEVTRRPEGNENPELATGEVEVTAEELEVLSEAAPLPLPVDDQIEAGDDVRLR 120 E VR+ G+V RRPEG EN +LATG +E+ + EVL +A PLPLP+D+ E + RLR Sbjct: 70 EAAVRIEGQVARRPEGTENTDLATGAIELRVQHAEVLGDADPLPLPLDEHAEVEETTRLR 129 Query: 121 YRYLDLRRSGPASALRLRSAANQIARTVLHERDFLEIETPTLTRSTPEGARDFLVPVRLQ 180 +RYLDLRR+ LR R+A N R + + F+E+ETP L TPEGAR+FLVP R Q Sbjct: 130 WRYLDLRRARMQRNLRARAATNAAIRRAMEAQGFVEVETPLLWTPTPEGAREFLVPSRTQ 189 Query: 181 PGTWYALPQSPQLFKQLLMVGGMERYYQIARCYRDEDFRADRQPEFTQLDIEMSFVTEDD 240 PG+ Y+LPQSPQ+ KQLLM+GG++RYYQIARC RDED RADRQ EFTQLD+E SFVT++D Sbjct: 190 PGSVYSLPQSPQIAKQLLMIGGVDRYYQIARCLRDEDLRADRQFEFTQLDLEASFVTQED 249 Query: 241 VIDLGEAIVSRLWRELAGHQITRPIPRITWHEAMARYGSDKPDLRYGVELTELTDYLRGT 300 V A + + R + G PI ITWHEAM R+GSDKPD R+G+EL ++T+ +R + Sbjct: 250 VRSFVSAAIREVIRSVRGVDDV-PIGTITWHEAMTRWGSDKPDRRFGMELVDVTEAVRDS 308 Query: 301 RFRVFAGAIEAGGYVGAVVMPGGAAQSRKELDGWQDWAKARGAKGLAYVVLDAETG-EAR 359 R A YV A+V+ SR LD A++ GAKGLA+ +++ T E Sbjct: 309 GARAL-----AADYVTALVV--AEPLSRSRLDELTQRARSAGAKGLAWFRVESRTPLEIA 361 Query: 360 GPVAKNLSTEHLAGLADVVGAKPGDAIFFAAGAESRAAQELLGAARVEIARRANLVDESA 419 P+AK ++ L L + GA+ GD I A A LG R+ + R +L D A Sbjct: 362 SPLAKAMTPAALEALVERTGAEVGDTILVVAD-RFEVACTALGTIRLALGR--HLADPDA 418 Query: 420 WAFCWVVDAPMFERVTDREEAGAGGWTAVHHPFTAPNAEWMDRFEEAPDRALAYAYDIVC 479 WV+D P+FE V D G + HHPFT P+A+ MD E P R + +YD+V Sbjct: 419 LDVFWVIDFPLFEGVDDE-----GHLVSAHHPFTMPHADDMDLLEREPLRVRSQSYDLVM 473 Query: 480 NGNEIGGGSIRIHRGDVQQRVFDLLGITPEQARDKFGFLLEAFKYGPPPHGGIAFGWDRV 539 NG E+G GSIRIHR D+Q ++F LLG+ + A D+FGFLL+AF++GPPPHGG AFG DR+ Sbjct: 474 NGWELGSGSIRIHRPDLQAQIFGLLGLDEQAAADRFGFLLDAFRFGPPPHGGFAFGIDRL 533 Query: 540 CMLLAGADSIREVIAFPKTRGGFDPLTAAPTPITAAQRLEAGVDAR 585 LL G ++IREVIAFPKT+ G DP+T AP P+ G++AR Sbjct: 534 VALLLGEETIREVIAFPKTQSGQDPMTGAPKPVDDGVLAALGLEAR 579 Lambda K H 0.319 0.137 0.415 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 886 Number of extensions: 44 Number of successful extensions: 8 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 607 Length of database: 588 Length adjustment: 37 Effective length of query: 570 Effective length of database: 551 Effective search space: 314070 Effective search space used: 314070 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 53 (25.0 bits)
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory