Alignments for a candidate for thrC in Acidimicrobium ferrooxidans DSM 10331

GapMind for Amino acid biosynthesis

Alignments for a candidate for thrC in Acidimicrobium ferrooxidans DSM 10331

Align Threonine synthase; TS; EC 4.2.3.1 (uncharacterized)
to candidate WP_015799239.1 AFER_RS09545 threonine synthase

Query= curated2:Q58860
         (405 letters)



>NCBI__GCF_000023265.1:WP_015799239.1
          Length = 414

 Score =  286 bits (731), Expect = 1e-81
 Identities = 150/381 (39%), Positives = 235/381 (61%), Gaps = 8/381 (2%)

Query: 5   CIKCGKTYDVDEIIYTCE-CGGLLEIIYDYEEIKDKVSEEKLRKREIGVWRYLEYLPVKD 63
           C +CG+TY +    + CE C G LE+ YDYE+I  +V+ E+++     +WRY E LP++ 
Sbjct: 10  CRECGRTYPISPT-HVCEFCFGPLEVAYDYEQIAARVTRERIQSGPPTLWRYGELLPIER 68

Query: 64  ESKIVSLCEGGTPLYRCNNLEKELGIKELYVKNEGANPTGSFKDRGMTVGVTRANELGVE 123
           +   + L  G TPL     L  ELG+ ELY+KN+  NPTGSFKDR + V +++A E G+ 
Sbjct: 69  DEARIDLGAGFTPLVPAPRLAAELGLGELYLKNDTVNPTGSFKDRVVAVALSKAREFGMR 128

Query: 124 VVGCASTGNTSASLAAYSARSGKKCIVLLPEGKVALGKLAQAMFYGAKVIQVKGNFDDAL 183
           V  CASTGN + S+AA++AR+G +  V +P     +  LA A+ YGA ++ + G +DD  
Sbjct: 129 VASCASTGNLANSVAAHAARAGMEAYVFIPANLERVKVLATAV-YGANIVAIDGTYDDVN 187

Query: 184 DMVKQLAKEKLIYLLNSIN--PFRLEGQKTIAFEICDQLNWQVPDRVIVPVGNAGNISAI 241
            +  ++  E+  +   ++N  PF  EG KT+ FE+ +QL W+ PD V+VPV +   ++ I
Sbjct: 188 RLCAEIVSERGDWGFANVNLRPFYSEGSKTLGFEVVEQLGWRYPDHVVVPVASGSQLTRI 247

Query: 242 WKGFKEFEITGIIDELP--KMTGIQADGAKPIVEAFRKRAKDIIPYKNPETIATAIRIGN 299
            +GF+E  + G++ E P  +++G QA G  P+ +AF +  + ++    P T+A ++ IGN
Sbjct: 248 HRGFEELALAGLMPEPPSVRVSGAQAQGCSPVAQAF-EAGRSLVRPVRPSTVAKSLAIGN 306

Query: 300 PVNAPKALDAIYSSGGYAEAVTDEEIVEAQKLLARKEGIFVEPASASSIAGLKKLLEEGI 359
           P +   A+D   ++GG   AV+D+EI+EA  L+AR EGIFVE A   ++A L KL  +GI
Sbjct: 307 PADGSYAVDVARATGGAIGAVSDDEILEAIALVARTEGIFVETAGGVTVATLAKLASQGI 366

Query: 360 IDRDERIVCITTGHGLKDPDA 380
           +  DE +V   +G+GLK  +A
Sbjct: 367 VRPDETVVAYLSGNGLKTVEA 387


Lambda     K      H
   0.317    0.137    0.395 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 413
Number of extensions: 20
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 405
Length of database: 414
Length adjustment: 31
Effective length of query: 374
Effective length of database: 383
Effective search space:   143242
Effective search space used:   143242
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 50 (23.9 bits)

Align candidate WP_015799239.1 AFER_RS09545 (threonine synthase)
to HMM TIGR00260 (thrC: threonine synthase (EC 4.2.3.1))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00260.hmm
# target sequence database:        /tmp/gapView.37861.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00260  [M=340]
Accession:   TIGR00260
Description: thrC: threonine synthase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                             Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                             -----------
    3.8e-93  298.3   0.0    4.4e-93  298.1   0.0    1.0  1  NCBI__GCF_000023265.1:WP_015799239.1  


Domain annotation for each sequence (and alignments):
>> NCBI__GCF_000023265.1:WP_015799239.1  
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  298.1   0.0   4.4e-93   4.4e-93       2     340 .]      58     386 ..      57     386 .. 0.98

  Alignments for each domain:
  == domain 1  score: 298.1 bits;  conditional E-value: 4.4e-93
                             TIGR00260   2 vryreilevt.ekdlvdlaegstelfrspklaeevgaenlyvkelfhgPtlaFKDrglqfvavlltkalelgn 73 
                                           +ry e+l++  ++  +dl +g+t+l+ +p+la+e+g+ +ly+k++ ++Pt++FKDr+   vav+l+ka+e g+
  NCBI__GCF_000023265.1:WP_015799239.1  58 WRYGELLPIErDEARIDLGAGFTPLVPAPRLAAELGLGELYLKNDTVNPTGSFKDRV---VAVALSKAREFGM 127
                                           9*********9999*******************************************...************* PP

                             TIGR00260  74 etvlcAtsGdtgaaaaealagkanvkvvvLyPkgkispvkeklvtalaenakvlaikGdFDdaqdlvkeifed 146
                                            +  cA++G++++++a a+a++a+++++v++P+  ++    k++ + +++a+++ai+G++Dd+++l++ei+++
  NCBI__GCF_000023265.1:WP_015799239.1 128 RVASCASTGNLANSVA-AHAARAGMEAYVFIPAN-LE--RVKVLATAVYGANIVAIDGTYDDVNRLCAEIVSE 196
                                           ****************.****************9.89..99******************************** PP

                             TIGR00260 147 keklklnsvNsinparieaqktyafeiveqlgkespdkvvvpvp.sgnfgailkGflekkelg.....lpiek 213
                                           +  +++ +vN++ p++ e++kt+ fe+veqlg++ pd+vvvpv+ ++ ++ i++Gf e+  +g       ++ 
  NCBI__GCF_000023265.1:WP_015799239.1 197 RGDWGFANVNLR-PFYSEGSKTLGFEVVEQLGWRYPDHVVVPVAsGSQLTRIHRGFEELALAGlmpepPSVRV 268
                                           **********88.*******************************7789***************98876669** PP

                             TIGR00260 214 laiaaegaadivrrflksgdlepkedkeTlstAmdignpsnveralelarrslgnledlkesvsdeeileaik 286
                                           ++++a+g+++++++f  ++ l+   +++T++++++ignp++  +a+++ar ++g + ++    sd+eileai 
  NCBI__GCF_000023265.1:WP_015799239.1 269 SGAQAQGCSPVAQAFEAGRSLVRPVRPSTVAKSLAIGNPADGSYAVDVARATGGAIGAV----SDDEILEAIA 337
                                           ***************9999****************************************....********** PP

                             TIGR00260 287 klaeeegyllephtavavaalkklvekgvsatadpaKFeevvealtgnklkdpe 340
                                           ++a++eg+++e++++v+va l kl+ +g    + p+  e+vv++l+gn+lk+ e
  NCBI__GCF_000023265.1:WP_015799239.1 338 LVARTEGIFVETAGGVTVATLAKLASQG---IVRPD--ETVVAYLSGNGLKTVE 386
                                           ****************************...*****..************9865 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (340 nodes)
Target sequences:                          1  (414 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.00u 0.00s 00:00:00.00 Elapsed: 00:00:00.00
# Mc/sec: 17.82
//
[ok]

This GapMind analysis is from Jul 25 2024. The underlying query database was built on Jul 25 2024.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis