Align Aspartate--tRNA(Asp/Asn) ligase; Aspartyl-tRNA synthetase; AspRS; Non-discriminating aspartyl-tRNA synthetase; ND-AspRS; EC 6.1.1.23 (characterized)
to candidate WP_015817601.1 TERTU_RS03770 aspartate--tRNA ligase
Query= SwissProt::Q51422 (591 letters) >NCBI__GCF_000023025.1:WP_015817601.1 Length = 600 Score = 852 bits (2202), Expect = 0.0 Identities = 405/589 (68%), Positives = 488/589 (82%) Query: 2 MRSHYCGQLNESLDGQEVTLCGWVHRRRDHGGVIFLDVRDREGLAQVVFDPDRAETFAKA 61 MRS YCG L + G+EVTLCGWV RRRDHGGVIF+D+RDR+G+ QVVFDPD + FA A Sbjct: 1 MRSVYCGVLTAANIGEEVTLCGWVDRRRDHGGVIFVDLRDRDGIVQVVFDPDAEDNFALA 60 Query: 62 DRVRSEFVVKITGKVRLRPEGARNPNMASGSIEVLGYELEVLNQAETPPFPLDEYSDVGE 121 D+VR E+V+++TGKVR R E N NMA+G +E+ G L +LN A+TPPF LD ++ VGE Sbjct: 61 DKVRPEYVLQVTGKVRARSESTVNANMATGEVEIYGTGLTILNTADTPPFQLDSFTSVGE 120 Query: 122 ETRLRYRFIDLRRPEMAAKLKLRARITSSIRRYLDDNGFLDVETPILGRPTPEGARDYLV 181 + RLRYR++DLRR EM L+ R+++T++IR YLD++GFLD+ETPIL R TPEGARDYLV Sbjct: 121 DVRLRYRYLDLRRKEMQHNLRFRSKVTNAIRNYLDEHGFLDIETPILTRATPEGARDYLV 180 Query: 182 PSRTYPGHFFALPQSPQLFKQLLMVAGFDRYYQIAKCFRDEDLRADRQPEFTQIDIETSF 241 PSRT+ FFALPQSPQLFKQLLMV+GFDRYYQIAKCFRDEDLRADRQPEFTQIDIETSF Sbjct: 181 PSRTHESKFFALPQSPQLFKQLLMVSGFDRYYQIAKCFRDEDLRADRQPEFTQIDIETSF 240 Query: 242 LDESDIIGITEKMVRQLFKEVLDVEFDEFPHMPFEEAMRRYGSDKPDLRIPLELVDVADQ 301 +DE I+ +TE M+R+LF +VL ++ +FP MP+ EAM +YGSDKPD+RIPLE+V++ D Sbjct: 241 MDEEQIMSVTEGMIRKLFNDVLSIDLGDFPRMPYSEAMEKYGSDKPDMRIPLEMVEIKDL 300 Query: 302 LKEVEFKVFSGPANDPKGRVAALRVPGAASMPRSQIDDYTKFVGIYGAKGLAYIKVNERA 361 +K+VEFKVFS PANDPK RV A+RVP +PR +ID YTKFV IYGAKGLAYIKVN++ Sbjct: 301 MKDVEFKVFSAPANDPKSRVTAMRVPQGGEIPRKKIDAYTKFVSIYGAKGLAYIKVNDKD 360 Query: 362 KGVEGLQSPIVKFIPEANLNVILDRVGAVDGDIVFFGADKAKIVCDALGALRIKVGHDLK 421 GLQSPIVKF+P IL+RVGA +GD++FFGAD K+V +ALGALR K+G DL Sbjct: 361 DLENGLQSPIVKFLPAEVCKAILERVGAENGDLIFFGADTTKVVTEALGALRCKLGEDLD 420 Query: 422 LLTREWAPMWVVDFPMFEENDDGSLSALHHPFTSPKCTPAELEANPGAALSRAYDMVLNG 481 L T EWAP+WVVDFPMFEE D+G L+ALHHPFT+P C+P +L+ANP ALSRAYDMVLNG Sbjct: 421 LYTCEWAPLWVVDFPMFEETDEGGLTALHHPFTAPSCSPEDLQANPATALSRAYDMVLNG 480 Query: 482 TELGGGSIRIHDKSMQQAVFRVLGIDEAEQEEKFGFLLDALKYGAPPHGGLAFGLDRLVM 541 TELGGGS+RIH++ MQQAVF++LGI EQ EKFGFLLDALK+GAPPHGGLAFGLDRL+M Sbjct: 481 TELGGGSVRIHNQDMQQAVFKILGISPEEQREKFGFLLDALKFGAPPHGGLAFGLDRLIM 540 Query: 542 LMTGASSIREVIAFPKTQSAGDVMTQAPGSVDGKALRELHIRLREQPKA 590 LMTGA SIR+VIAFPKTQSA +MT APG+VD K L+ELHIRLR +P+A Sbjct: 541 LMTGADSIRDVIAFPKTQSAACLMTDAPGAVDAKQLQELHIRLRAKPQA 589 Lambda K H 0.321 0.140 0.411 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1120 Number of extensions: 35 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 591 Length of database: 600 Length adjustment: 37 Effective length of query: 554 Effective length of database: 563 Effective search space: 311902 Effective search space used: 311902 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 53 (25.0 bits)
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory