Align Dihydroxy-acid dehydratase; DAD; EC 4.2.1.9 (characterized)
to candidate WP_015928511.1 MNOD_RS08820 dihydroxy-acid dehydratase
Query= SwissProt::Q1MIB2 (612 letters) >NCBI__GCF_000022085.1:WP_015928511.1 Length = 609 Score = 978 bits (2529), Expect = 0.0 Identities = 488/609 (80%), Positives = 528/609 (86%) Query: 1 MPVYRSRTTTHGRNMAGARGLWRATGMKDSDFGKPIIAVVNSFTQFVPGHVHLKDLGQLV 60 MP YRSRTTTHGRNMAGARGLWRATGMKD+DFGKPIIAVVNSFTQFVPGHVHLKDLGQLV Sbjct: 1 MPTYRSRTTTHGRNMAGARGLWRATGMKDADFGKPIIAVVNSFTQFVPGHVHLKDLGQLV 60 Query: 61 AREIEAAGGVAKEFNTIAVDDGIAMGHDGMLYSLPSRELIADSVEYMVNAHCADAMVCIS 120 AREIE AGGVAKEFNTIAVDDGIAMGHDGMLYSLPSRELIADSVEYMVNAHCADAMVCIS Sbjct: 61 AREIEQAGGVAKEFNTIAVDDGIAMGHDGMLYSLPSRELIADSVEYMVNAHCADAMVCIS 120 Query: 121 NCDKITPGMLMASLRLNIPTVFVSGGPMEAGKVVMHGKTHALDLVDAMVAAADDKISDED 180 NCDKITPGMLMA+LRLNIP VFVSGGPMEAGKVV+ G LDLVDAMVAAADD++ D D Sbjct: 121 NCDKITPGMLMAALRLNIPAVFVSGGPMEAGKVVLGGARKKLDLVDAMVAAADDRVGDAD 180 Query: 181 VQTIERSACPTCGSCSGMFTANSMNCLTEALGLSLPGNGSTLATHLDRKRLFVEAGHLIV 240 +Q IERSACPTCGSCSGMFTANSMNCLTEALGLSLPGNGS LATH DR+RLFVEAGHLIV Sbjct: 181 LQVIERSACPTCGSCSGMFTANSMNCLTEALGLSLPGNGSVLATHADRRRLFVEAGHLIV 240 Query: 241 DLARRYYEQDDVKALPRTIASKQAFENAMTLDIAMGGSTNTVLHILAAAHEGEIDFTMAD 300 DLARRYYEQDD LPR IAS AFENAMTLDIAMGGSTNTVLH+LAAAHE + F+M D Sbjct: 241 DLARRYYEQDDDSVLPRAIASFAAFENAMTLDIAMGGSTNTVLHLLAAAHEAGVPFSMTD 300 Query: 301 IDALSRRVPCLSKVAPAKSDVHMEDVHRAGGIMSILGELDKGGLLNRDCPTVHAETLGDA 360 ID LSRRVP L KVAPA DVHMEDVHRAGGIM ILGELD+ GLL+ +VHAE+L A Sbjct: 301 IDRLSRRVPVLCKVAPAVPDVHMEDVHRAGGIMGILGELDRAGLLDTSVGSVHAESLAAA 360 Query: 361 IDRWDITRTNSETVRKFYRAAPGGIPTQVAFSQEARWDELDTDRENGVIRSVEHPFSKDG 420 ++RWD+ R++S TV FYRAAPGG+PTQ AFSQEAR+D LD DR GV+R EH +++DG Sbjct: 361 LERWDVRRSDSPTVHAFYRAAPGGVPTQTAFSQEARFDALDLDRAGGVVRDAEHAYARDG 420 Query: 421 GLAVLKGNLAIDGCIVKTAGVDESILKFSGPARVFESQDASVKAILANEVKAGDVVVIRY 480 GLAVL GNLA DGCIVKTAGVD SIL F+GPA VFESQDA+V IL+ VKAG+VV+IRY Sbjct: 421 GLAVLYGNLATDGCIVKTAGVDASILAFAGPAHVFESQDAAVDGILSGRVKAGEVVLIRY 480 Query: 481 EGPKGGPGMQEMLYPTSYLKSKGLGKACALITDGRFSGGTSGLSIGHASPEAANGGTIGL 540 EGP+GGPGMQEMLYPTSYLKSKGLGKACAL+TDGRFSGG+SGLSIGH SPEAA GG IGL Sbjct: 481 EGPRGGPGMQEMLYPTSYLKSKGLGKACALVTDGRFSGGSSGLSIGHVSPEAAEGGLIGL 540 Query: 541 VREGDMIDIDIPNRTISLRVSETELAARRAEQDAKGWYPTEVRKRNVTTALKAYAAFATS 600 V +GD I+IDIPNR I L V E EL RRA Q+A+GW P RKR V+ AL+AYA TS Sbjct: 541 VEQGDRIEIDIPNRRIHLAVDEAELERRRAAQEARGWQPAVPRKRKVSAALRAYAMLTTS 600 Query: 601 ADRGAVRDL 609 A +GAVR L Sbjct: 601 AAQGAVRKL 609 Lambda K H 0.318 0.134 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1307 Number of extensions: 45 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 612 Length of database: 609 Length adjustment: 37 Effective length of query: 575 Effective length of database: 572 Effective search space: 328900 Effective search space used: 328900 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 53 (25.0 bits)
Align candidate WP_015928511.1 MNOD_RS08820 (dihydroxy-acid dehydratase)
to HMM TIGR00110 (ilvD: dihydroxy-acid dehydratase (EC 4.2.1.9))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00110.hmm # target sequence database: /tmp/gapView.15700.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00110 [M=543] Accession: TIGR00110 Description: ilvD: dihydroxy-acid dehydratase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 1.8e-251 821.3 1.5 2e-251 821.1 1.5 1.0 1 lcl|NCBI__GCF_000022085.1:WP_015928511.1 MNOD_RS08820 dihydroxy-acid dehy Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_000022085.1:WP_015928511.1 MNOD_RS08820 dihydroxy-acid dehydratase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 821.1 1.5 2e-251 2e-251 2 542 .. 18 608 .. 17 609 .] 0.99 Alignments for each domain: == domain 1 score: 821.1 bits; conditional E-value: 2e-251 TIGR00110 2 arallkatGlkdedlekPiiavvnsyteivPghvhlkdlaklvkeeieaaGgvakefntiavsDGiamg 70 ar l++atG+kd+d++kPiiavvns+t++vPghvhlkdl++lv++eie+aGgvakefntiav+DGiamg lcl|NCBI__GCF_000022085.1:WP_015928511.1 18 ARGLWRATGMKDADFGKPIIAVVNSFTQFVPGHVHLKDLGQLVAREIEQAGGVAKEFNTIAVDDGIAMG 86 689****************************************************************** PP TIGR00110 71 heGmkysLpsreiiaDsvetvvkahalDalvvissCDkivPGmlmaalrlniPaivvsGGpmeagktkl 139 h+Gm+ysLpsre+iaDsve++v+ah++Da+v+is+CDki+PGmlmaalrlniPa++vsGGpmeagk+ l lcl|NCBI__GCF_000022085.1:WP_015928511.1 87 HDGMLYSLPSRELIADSVEYMVNAHCADAMVCISNCDKITPGMLMAALRLNIPAVFVSGGPMEAGKVVL 155 ********************************************************************* PP TIGR00110 140 sekidlvdvfeavgeyaagklseeeleeiersacPtagsCsGlftansmacltealGlslPgsstllat 208 + +++d+++a++++a++++ +++l+ iersacPt+gsCsG+ftansm+cltealGlslPg++++lat lcl|NCBI__GCF_000022085.1:WP_015928511.1 156 GGARKKLDLVDAMVAAADDRVGDADLQVIERSACPTCGSCSGMFTANSMNCLTEALGLSLPGNGSVLAT 224 ********************************************************************* PP TIGR00110 209 saekkelakksgkrivelvkknik.......PrdiltkeafenaitldlalGGstntvLhllaiakeag 270 +a++++l+ ++g+ iv+l +++++ Pr i++ +afena+tld+a+GGstntvLhlla+a+eag lcl|NCBI__GCF_000022085.1:WP_015928511.1 225 HADRRRLFVEAGHLIVDLARRYYEqdddsvlPRAIASFAAFENAMTLDIAMGGSTNTVLHLLAAAHEAG 293 ********************************************************************* PP TIGR00110 271 vklslddfdrlsrkvPllaklkPsgkkv.iedlhraGGvsavlkeldkegllhkdaltvtGktlaetle 338 v +s+ d+drlsr+vP+l+k++P+ v +ed+hraGG++++l+eld++gll++ + +v ++la le lcl|NCBI__GCF_000022085.1:WP_015928511.1 294 VPFSMTDIDRLSRRVPVLCKVAPAVPDVhMEDVHRAGGIMGILGELDRAGLLDTSVGSVHAESLAAALE 362 ************************98888**************************************** PP TIGR00110 339 kvkvlr.................................vdqd....virsldnpvkkegglavLkGnl 370 + +v+r + d v+r ++++ ++gglavL+Gnl lcl|NCBI__GCF_000022085.1:WP_015928511.1 363 RWDVRRsdsptvhafyraapggvptqtafsqearfdaldL--DraggVVRDAEHAYARDGGLAVLYGNL 429 **********************************999663..33467********************** PP TIGR00110 371 aeeGavvkiagveedilkfeGpakvfeseeealeailggkvkeGdvvviryeGPkGgPGmremLaPtsa 439 a++G++vk+agv+ +il f Gpa+vfes+++a+++il+g+vk+G+vv+iryeGP+GgPGm+emL+Pts+ lcl|NCBI__GCF_000022085.1:WP_015928511.1 430 ATDGCIVKTAGVDASILAFAGPAHVFESQDAAVDGILSGRVKAGEVVLIRYEGPRGGPGMQEMLYPTSY 498 ********************************************************************* PP TIGR00110 440 lvglGLgkkvaLitDGrfsGgtrGlsiGhvsPeaaegGaialvedGDkikiDienrkldlevseeelae 508 l++ GLgk++aL+tDGrfsGg++GlsiGhvsPeaaegG i+lve+GD+i+iDi+nr+++l+v+e+el++ lcl|NCBI__GCF_000022085.1:WP_015928511.1 499 LKSKGLGKACALVTDGRFSGGSSGLSIGHVSPEAAEGGLIGLVEQGDRIEIDIPNRRIHLAVDEAELER 567 ********************************************************************* PP TIGR00110 509 rrakakkkea.......revkgaLakyaklvssadkGavld 542 rra+++++++ r+v+ aL++ya l +sa +Gav++ lcl|NCBI__GCF_000022085.1:WP_015928511.1 568 RRAAQEARGWqpavprkRKVSAALRAYAMLTTSAAQGAVRK 608 *********99****9999********************86 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (543 nodes) Target sequences: 1 (609 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.02u 0.01s 00:00:00.03 Elapsed: 00:00:00.03 # Mc/sec: 10.12 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory