Alignments for a candidate for asnB in Methylobacterium nodulans ORS 2060

GapMind for Amino acid biosynthesis

Alignments for a candidate for asnB in Methylobacterium nodulans ORS 2060

Align asparagine synthase (glutamine-hydrolysing) (EC 6.3.5.4) (characterized)
to candidate WP_015933298.1 MNOD_RS33010 asparagine synthetase B

Query= BRENDA::Q9SP20
         (591 letters)



>NCBI__GCF_000022085.1:WP_015933298.1
          Length = 470

 Score =  160 bits (405), Expect = 1e-43
 Identities = 141/467 (30%), Positives = 213/467 (45%), Gaps = 46/467 (9%)

Query: 1   MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLYQHGDNYLSHQRLAIIDPASG- 59
           MCGI+          + R+       +L HRGPD SG+   G     H RL+++D  S  
Sbjct: 1   MCGIIGSFNLPGFDVSSRLE------QLAHRGPDGSGVLSAGPAVHGHVRLSLVDLTSAS 54

Query: 60  DQPLFNEDQTIVVTVNGEIYNHEELRKRLTAAGHKFRTGSDCDVIAHLYEEHGEDFVDML 119
           DQP    D   V+T NGEI+N++E+R +L A G +FRT  D +V+A +    G   +  L
Sbjct: 55  DQPFRYGDA--VLTFNGEIWNYQEVRAQLQAEGVRFRTAGDTEVLAAVLHRWGVHGLARL 112

Query: 120 DGMFSFVLLDTRNNTYIAARDAIGITSLYIGWGLDGSVWISSELKGLNDDCEHFEVFPPG 179
           +GMF+F    ++   +I  RD  G   LY+    +G  W SSE KGL  DC    + PPG
Sbjct: 113 EGMFAFAW--SKGEMHILVRDRFGEIPLYVHRKGNGFAW-SSERKGLGRDCPAAPL-PPG 168

Query: 180 HLYSSKTGGFKRWYNPLWFAETIPSTPYDPLVLRCAFENAVIKRLMTDVPFGVLLSGGLD 239
            +     G  + WY         P  P     L     + V +RL+ D P   L+SGGLD
Sbjct: 169 CVLDLPNGEVRPWYER-------PEHPGINDRLISLVRDGVERRLVADAPLCCLISGGLD 221

Query: 240 SSLVASITARYLAGTKAAKQWGAQLHSFCVGLEG-SPDLKAAREVADYLGTVHHEFHFTV 298
           SSLV       LA  KA K     + ++   L+G S DL+AAR + D       E     
Sbjct: 222 SSLV-------LALAKARK---PDVVAYTAVLDGESADLRAARRLCDEFEVPLVEVPVPA 271

Query: 299 QDGIDAIEDVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVISGEGSDEIFGGY-LYF 357
             G DA+E     IE      +  +     ++  I S G K  +SGE +DE+FGGY    
Sbjct: 272 PTG-DALEAAARAIEIDSKAQVEIAALCIPLAHAIASDGFKACLSGEAADELFGGYGNMC 330

Query: 358 HKALNREEFHQETCR--KIKALHQYDCLRANKSTSAWGLEARVPFLDKEFINVAMSIDPE 415
            K  +  +      R  ++  + + + +R NK+  A G+E R+PF+++  +   +++   
Sbjct: 331 IKGASASDIGWREIRVAQLLKMARGNFVRCNKAFMAAGVECRLPFIERRLVEAVIAM--- 387

Query: 416 AKMINMDQKRIEKWVLRRAFEDEEHPYLPKHILYRQKEQFSDGVGYS 462
                    + E    + A +      +P  ++ R K+ F  G G S
Sbjct: 388 --------TKAECPPGKGALKQAAAGIVPGWVVKRPKDTFQGGAGMS 426


Lambda     K      H
   0.320    0.137    0.421 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 584
Number of extensions: 29
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 591
Length of database: 470
Length adjustment: 35
Effective length of query: 556
Effective length of database: 435
Effective search space:   241860
Effective search space used:   241860
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis