Align Probable acetolactate synthase large subunit; AHAS; EC 2.2.1.6; Acetohydroxy-acid synthase large subunit; ALS (uncharacterized)
to candidate WP_015943252.1 DHAF_RS05900 thiamine pyrophosphate-binding protein
Query= curated2:O08353 (599 letters) >NCBI__GCF_000021925.1:WP_015943252.1 Length = 591 Score = 372 bits (956), Expect = e-107 Identities = 212/592 (35%), Positives = 338/592 (57%), Gaps = 47/592 (7%) Query: 1 MNGAEAMIKALEAEKVEILFGYPGGALLPFYDALHHSDLIHLLTRHEQAAAHAADGYARA 60 M GA+ + L + V ++G PG A+LPFYDA+ ++ RHEQAA ADGY+RA Sbjct: 24 MTGAQFLAAFLAKKGVSQIYGIPGAAILPFYDAVRELNMESYNVRHEQAAIFMADGYSRA 83 Query: 61 SGKVGVCIGTSGPGATNLVTGVATAHSDSSPMVALTGQVPTKLIGNDAFQEIDALGLFMP 120 +GKVGVC TSGPGATN +TG+ +A+SDS P++ALTGQVPT LIG D+FQE + + P Sbjct: 84 TGKVGVCAATSGPGATNFLTGLYSAYSDSVPLLALTGQVPTSLIGKDSFQEAPMVEMARP 143 Query: 121 IVKHNFQIQKTCQIPEIFRSAFEIAQTGRPGPVHIDLPKDVQELELDID------KHPIP 174 + K + ++K +P I + +E+A TG+ GP+ +DLP DVQ+ +++D P P Sbjct: 144 VTKAAYLVEKAGDLPRILKEGWELATTGKKGPILLDLPLDVQKGLVEVDWSEFDFSPPQP 203 Query: 175 SKVKLIGYNPTTIGHPRQIKKAIKLIASAKRPIILAGGGVLLSGANEELLKLVELLNIPV 234 + ++ TI + ++ AK P +LAGGG++ A E L + ELL IPV Sbjct: 204 PRQEIAQDTLNTI---------LSMLYEAKTPTLLAGGGIVQGNAAELLRETAELLQIPV 254 Query: 235 CTTLMGKGCISENHPLALGMVG-MHGTKPANYCLSESDVLISIGCRFSDRITGDIKSFAT 293 ++LMGK +HPL G+VG M T N + ESD++I++G RF R TGD + F + Sbjct: 255 VSSLMGKDAFPNDHPLYGGLVGTMCQTPLGNKTILESDLIINLGGRFDGRGTGDSQRFQS 314 Query: 294 NAKIIHIDIDPAEIGKNVNVDVPIVGDAKLILKEVIKQLDYIINKDSKENNDKENI--SQ 351 K++H+++D E+ ++++ ++ + D + L+ + L DK I Q Sbjct: 315 GRKVVHVNLDNRELSRHISTELAVAADIRDFLEHFLAFL-----------RDKGFIPTPQ 363 Query: 352 WIENVNSLKKSSIPV---MDYDDIPIKPQKIVKELMAVIDDLNINKNTIITTDVGQNQMW 408 + + L++ I + +D +P+KPQ+ + E+ + ++ I+T D G +Q+W Sbjct: 364 ARQRILDLQEERIRLARRTKFDTLPLKPQQAIAEVREA-----MARDGILTLDCGISQIW 418 Query: 409 MAHYFKTQTPRSFLSSGGLGTMGFGFPSAIGAKVAKPDSKVICITGDGGFMMNCQELGTI 468 F+ PRSFL +G GTMG+G +A+GAK+A PD +V+ + GD M+ QEL T Sbjct: 419 ATQLFEAYVPRSFLITGRAGTMGWGLGAALGAKLAYPDRQVVNLLGDASLGMSLQELATA 478 Query: 469 AEYNIPVVICIFDNRTLGMVYQWQNLFYGKRQCSVNF---------GGAPDFIKLAESYG 519 A++NIPVV+ + +N G++ Q QNL + +R S + DF+ A G Sbjct: 479 AKHNIPVVVVVLNNSLFGLIRQQQNLLFEQRCISTDLEYENHVQGHSRGLDFVATARGMG 538 Query: 520 IKARRIESPNEINEALKEAINCDEPYLLDFAIDPSSALSMVPPGAKLTNIID 571 ++A ++ P++I AL A + + PYL++ +DP + S+ G LT +++ Sbjct: 539 VEAELVDGPDQIQGALARAFSQNRPYLIEVLVDPLAQCSVSLDGT-LTGVLE 589 Lambda K H 0.319 0.137 0.405 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 752 Number of extensions: 37 Number of successful extensions: 6 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 599 Length of database: 591 Length adjustment: 37 Effective length of query: 562 Effective length of database: 554 Effective search space: 311348 Effective search space used: 311348 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 53 (25.0 bits)
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory