Align Gamma-glutamyl phosphate reductase; GPR; Glutamate-5-semialdehyde dehydrogenase; Glutamyl-gamma-semialdehyde dehydrogenase; GSA dehydrogenase; EC 1.2.1.41 (characterized)
to candidate WP_015944065.1 DHAF_RS12410 gamma-glutamyl-phosphate reductase
Query= SwissProt::P07004 (417 letters) >NCBI__GCF_000021925.1:WP_015944065.1 Length = 417 Score = 370 bits (950), Expect = e-107 Identities = 188/408 (46%), Positives = 276/408 (67%), Gaps = 5/408 (1%) Query: 5 MGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILNANAQDVADARANGLSEAMLD 64 +G AK A+ KLA + KN+ L +A+ L + IL AN +DV A G +++++ Sbjct: 10 IGQKAKDAARKLAYAGTAAKNKALLAMAEALLNHEQKILEANRKDVEAAIQKGTKKSLVN 69 Query: 65 RLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSGLRLERRRVPLGVIGVIYEARPN 124 RLALT ++ ++D +++V NL DPVG+ + +GLR++R RVPLGV+ +IYEARPN Sbjct: 70 RLALTSEGIRQMSDALKEVVNLGDPVGEG-EFWTRPNGLRIQRTRVPLGVVAMIYEARPN 128 Query: 125 VTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQDALKSCGLPAGAVQAIDNPDRALV 184 VTVD A+LCLK+GNAVILRGG E +N VI A +S G+P +Q ++N +R V Sbjct: 129 VTVDAAALCLKSGNAVILRGGSEAIESNKILSKVIAGAAESQGMPTACIQLLENTNRQWV 188 Query: 185 SEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVITGGIGVCHIYVDESVEIAEALKVIVN 244 ++++M+ Y+D++IPRGGAGL + +++T+PVI G GVCH YVD ++A+ + ++ N Sbjct: 189 QQLMKMNGYVDVIIPRGGAGLIETVVKEATVPVIETGTGVCHAYVDGEADLAKGVSIVFN 248 Query: 245 AKTQRPSTCNTVETLLVNKNIADSFLPALSKQMAESGVTLHADAAALAQLQAGPAKVVAV 304 AKTQ+P CN +E +LVN+ +A FLP L ++ + GV + A L Sbjct: 249 AKTQKPGVCNALEAVLVNEAVAQEFLPLLGEKFRDYGVEIRGCEKTCAIL----PYAAKA 304 Query: 305 KAEEYDDEFLSLDLNVKIVSDLDDAIAHIREHGTQHSDAILTRDMRNAQRFVNEVDSSAV 364 K E++ E L L ++ K+V +D+A+ HI ++GT+HS+ I+T + AQRF+NEVD++AV Sbjct: 305 KEEDWGIEHLDLIISAKVVQGVDEAMDHIYQYGTKHSETIITENYTTAQRFLNEVDAAAV 364 Query: 365 YVNASTRFTDGGQFGLGAEVAVSTQKLHARGPMGLEALTTYKWIGIGD 412 YVNASTRFTDGG+FG GAE+ +STQKLHARGPMGL+ALTT K++ G+ Sbjct: 365 YVNASTRFTDGGRFGFGAEIGISTQKLHARGPMGLQALTTMKYMVYGE 412 Lambda K H 0.317 0.133 0.369 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 402 Number of extensions: 14 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 417 Length of database: 417 Length adjustment: 31 Effective length of query: 386 Effective length of database: 386 Effective search space: 148996 Effective search space used: 148996 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 50 (23.9 bits)
Align candidate WP_015944065.1 DHAF_RS12410 (gamma-glutamyl-phosphate reductase)
to HMM TIGR00407 (proA: glutamate-5-semialdehyde dehydrogenase (EC 1.2.1.41))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00407.hmm # target sequence database: /tmp/gapView.6868.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00407 [M=398] Accession: TIGR00407 Description: proA: glutamate-5-semialdehyde dehydrogenase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 3.6e-158 512.5 1.7 4.1e-158 512.3 1.7 1.0 1 lcl|NCBI__GCF_000021925.1:WP_015944065.1 DHAF_RS12410 gamma-glutamyl-phos Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_000021925.1:WP_015944065.1 DHAF_RS12410 gamma-glutamyl-phosphate reductase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 512.3 1.7 4.1e-158 4.1e-158 1 398 [] 14 406 .. 14 406 .. 0.99 Alignments for each domain: == domain 1 score: 512.3 bits; conditional E-value: 4.1e-158 TIGR00407 1 akeaalklaqlstaeknealskiadeLkaeaelilaanakdiaaakenGladalldrLlLteeklksia 69 ak+aa kla ta kn+al ++a++L ++ + il+an+kd++aa ++G ++l++rL+Lt e ++++ lcl|NCBI__GCF_000021925.1:WP_015944065.1 14 AKDAARKLAYAGTAAKNKALLAMAEALLNHEQKILEANRKDVEAAIQKGTKKSLVNRLALTSEGIRQMS 82 89******************************************************************* PP TIGR00407 70 ddvkdvieLadPvGkvieareldeGLklervrvPlGvlgviyearPevivdvasLclktGnaviLkGgk 138 d++k+v++L dPvG+ e ++ +GL+++r rvPlGv+ +iyearP+v+vd+a+Lclk+GnaviL+Gg+ lcl|NCBI__GCF_000021925.1:WP_015944065.1 83 DALKEVVNLGDPVGEG-EFWTRPNGLRIQRTRVPLGVVAMIYEARPNVTVDAAALCLKSGNAVILRGGS 150 **************97.567889********************************************** PP TIGR00407 139 eavrsnkalveviqdaleqtglpveavqliedpdreevkellkldeyvdlliPrGgnelvklikeesti 207 ea++snk+l +vi a e+ g+p+ ++ql+e+++r+ v++l+k++ yvd++iPrGg++l++ + +e+t+ lcl|NCBI__GCF_000021925.1:WP_015944065.1 151 EAIESNKILSKVIAGAAESQGMPTACIQLLENTNRQWVQQLMKMNGYVDVIIPRGGAGLIETVVKEATV 219 ********************************************************************* PP TIGR00407 208 PvlehadGvChiyldesadlakakkvivdaktqrPstCnaietLLvnkaiaeefleeLekqleekgvel 276 Pv+e+++GvCh y+d +adlak ++ +aktq+P +Cna+e++Lvn+a+a+efl+ L ++ + gve+ lcl|NCBI__GCF_000021925.1:WP_015944065.1 220 PVIETGTGVCHAYVDGEADLAKGVSIVFNAKTQKPGVCNALEAVLVNEAVAQEFLPLLGEKFRDYGVEI 288 ********************************************************************* PP TIGR00407 277 radalvlkllelekateaevskedfdkeflsldLsvkivedleeaiehirqygtkhsdailtedkknae 345 r+ ++++++l+ + a++++ed+ e+l+l++s+k+v+ ++ea++hi qygtkhs++i+te+ ++a+ lcl|NCBI__GCF_000021925.1:WP_015944065.1 289 RGCEKTCAILPYA----AKAKEEDWGIEHLDLIISAKVVQGVDEAMDHIYQYGTKHSETIITENYTTAQ 353 ***********99....677899********************************************** PP TIGR00407 346 kfvkevdsaavyvnastrfadGfrfGfGaevgistqklharGPvGLeaLvsyk 398 +f++evd+aavyvnastrf+dG+rfGfGae+gistqklharGP+GL+aL+++k lcl|NCBI__GCF_000021925.1:WP_015944065.1 354 RFLNEVDAAAVYVNASTRFTDGGRFGFGAEIGISTQKLHARGPMGLQALTTMK 406 **************************************************987 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (398 nodes) Target sequences: 1 (417 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.01 # Mc/sec: 9.84 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory