Alignments for a candidate for asd in Desulfitobacterium hafniense DCB-2

GapMind for Amino acid biosynthesis

Alignments for a candidate for asd in Desulfitobacterium hafniense DCB-2

Align aspartate semialdehyde dehydrogenase subunit (EC 1.2.1.11) (characterized)
to candidate WP_015944706.1 DHAF_RS18270 aspartate-semialdehyde dehydrogenase

Query= metacyc::MONOMER-6564
         (346 letters)



>NCBI__GCF_000021925.1:WP_015944706.1
          Length = 329

 Score =  339 bits (869), Expect = 7e-98
 Identities = 174/335 (51%), Positives = 238/335 (71%), Gaps = 9/335 (2%)

Query: 6   HVAVVGATGAVGQQMLKTLEDRNFEMDTLTLLSSKRSAGTKVTFKGQELTVQEASPESFE 65
           +VA+VGATGAVGQ+ LK L +RNF ++ L LL++KRSAG K+ ++G+E+ VQE + +SF+
Sbjct: 3   NVAIVGATGAVGQEFLKILVERNFPVEELRLLATKRSAGKKILWQGREIEVQETTHDSFK 62

Query: 66  GVNIALFSAGGSVSQALAPEAVKRGAIVIDNTSAFRMDENTPLVVPEVNEADLHEHNGII 125
           G++IALF AGGS S   AP AVK GA+VIDN+SA+R+D   PLVVPEVN  D+  H GII
Sbjct: 63  GIDIALF-AGGSSSTEFAPSAVKSGAVVIDNSSAYRLDPEVPLVVPEVNPEDVKWHKGII 121

Query: 126 ANPNCSTIQMVAALEPIRKAYGLNKVIVSTYQAVSGAGNEAVKELYSQTQAILNKEEIEP 185
           ANPNCSTI M  AL+PI    G+ +V+VSTYQAVSGAG E ++EL  Q +  +  EE++ 
Sbjct: 122 ANPNCSTIIMAVALKPIFDLAGIKRVVVSTYQAVSGAGREGIEELEDQVRNWVQGEEVKS 181

Query: 186 EIMPVKGDKKHYQIAFNAIPQIDKFQDNGYTFEEMKMINETKKIMHMPDLQVAATCVRLP 245
           +  P       YQIAFN IP+ID FQ+  YT EE KM+ ET+KI H+  + + AT VR+P
Sbjct: 182 QTFP-------YQIAFNLIPRIDVFQEGDYTKEEWKMVKETQKIFHVDTMPITATTVRVP 234

Query: 246 IQTGHSESVYIEIDRDDATVEDIKNLLKEAPGVTLQDDPSQQLYPMPADAVGKNDVFVGR 305
           +   HSES+ IE +R    ++++K   ++APGV ++D+P++  YPMP      ++V++GR
Sbjct: 235 VLRSHSESINIETER-SVGLDEVKAAFQKAPGVIVEDNPAEDRYPMPWFTSDTDEVYIGR 293

Query: 306 IRKDLDRANGFHLWVVSDNLLKGAAWNSVQIAESL 340
           IRKD    NG +LWVV D + KGAA N++QIAE L
Sbjct: 294 IRKDFSIENGINLWVVGDQIRKGAATNTIQIAELL 328


Lambda     K      H
   0.314    0.131    0.366 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 307
Number of extensions: 10
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 346
Length of database: 329
Length adjustment: 28
Effective length of query: 318
Effective length of database: 301
Effective search space:    95718
Effective search space used:    95718
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 49 (23.5 bits)

Align candidate WP_015944706.1 DHAF_RS18270 (aspartate-semialdehyde dehydrogenase)
to HMM TIGR01296 (asd: aspartate-semialdehyde dehydrogenase (EC 1.2.1.11))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR01296.hmm
# target sequence database:        /tmp/gapView.1275.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01296  [M=339]
Accession:   TIGR01296
Description: asd_B: aspartate-semialdehyde dehydrogenase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
   3.5e-144  466.0   0.1   3.9e-144  465.8   0.1    1.0  1  lcl|NCBI__GCF_000021925.1:WP_015944706.1  DHAF_RS18270 aspartate-semialdeh


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000021925.1:WP_015944706.1  DHAF_RS18270 aspartate-semialdehyde dehydrogenase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  465.8   0.1  3.9e-144  3.9e-144       1     336 [.       3     328 ..       3     329 .] 0.99

  Alignments for each domain:
  == domain 1  score: 465.8 bits;  conditional E-value: 3.9e-144
                                 TIGR01296   1 nvaivGatGavGqellkvLeernfpidklvllasersaGkkvkfkgkeleveeaekesfegidialfsa 69 
                                               nvaivGatGavGqe+lk+L ernfp+++l+lla +rsaGkk+  +g+e+ev+e+++ sf+gidialf a
  lcl|NCBI__GCF_000021925.1:WP_015944706.1   3 NVAIVGATGAVGQEFLKILVERNFPVEELRLLATKRSAGKKILWQGREIEVQETTHDSFKGIDIALF-A 70 
                                               79*****************************************************************.6 PP

                                 TIGR01296  70 GgsvskefapkaakagviviDntsafrldedvPLvvpevnaeelkeakkkgiianPnCstiqlvvvLkp 138
                                               Ggs s efap+a+k+g++viDn+sa+rld++vPLvvpevn e++k +k  giianPnCsti + v+Lkp
  lcl|NCBI__GCF_000021925.1:WP_015944706.1  71 GGSSSTEFAPSAVKSGAVVIDNSSAYRLDPEVPLVVPEVNPEDVKWHK--GIIANPNCSTIIMAVALKP 137
                                               8888*****************************************998..******************* PP

                                 TIGR01296 139 lkdeaklkrvvvstYqavsGaGkkgveeLknqtkavlegkekepeidalkakkfakqiafnaiplidkl 207
                                               + d a++krvvvstYqavsGaG++g+eeL++q++ +++g+e +       +++f++qiafn+ip id +
  lcl|NCBI__GCF_000021925.1:WP_015944706.1 138 IFDLAGIKRVVVSTYQAVSGAGREGIEELEDQVRNWVQGEEVK-------SQTFPYQIAFNLIPRIDVF 199
                                               ***************************************9987.......69***************** PP

                                 TIGR01296 208 kedGytkeelkllfetrkilgiedlkvsatcvrvPvftghsesvsiefekelsveevkelLkeapgvvv 276
                                               +e  ytkee k++ et+ki++++ ++++at+vrvPv+++hses++ie+e+ + ++evk  +++apgv+v
  lcl|NCBI__GCF_000021925.1:WP_015944706.1 200 QEGDYTKEEWKMVKETQKIFHVDTMPITATTVRVPVLRSHSESINIETERSVGLDEVKAAFQKAPGVIV 268
                                               ********************************************************************* PP

                                 TIGR01296 277 iddpsenlyptPleavgkdevfvgrirkDlskekglalfvvaDnlrkGaalnavqiaell 336
                                                d+p e+ yp+P  ++++dev++grirkD s e+g++l+vv+D++rkGaa+n++qiaell
  lcl|NCBI__GCF_000021925.1:WP_015944706.1 269 EDNPAEDRYPMPWFTSDTDEVYIGRIRKDFSIENGINLWVVGDQIRKGAATNTIQIAELL 328
                                               **********************************************************97 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (339 nodes)
Target sequences:                          1  (329 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01
# Mc/sec: 10.04
//
[ok]

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis