Align Ribose-phosphate pyrophosphokinase; RPPK; 5-phospho-D-ribosyl alpha-1-diphosphate synthase; Phosphoribosyl diphosphate synthase; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase; PRPPase; EC 2.7.6.1 (characterized)
to candidate WP_017364368.1 MCA_RS05175 ribose-phosphate diphosphokinase
Query= SwissProt::P0A1V6 (315 letters) >NCBI__GCF_000008325.1:WP_017364368.1 Length = 317 Score = 393 bits (1010), Expect = e-114 Identities = 196/308 (63%), Positives = 248/308 (80%) Query: 6 LFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQINENVRGGDIFIIQSTCAPTND 65 +F+GNA LA+ + +L LG A VGRFSDGE++V+I E+VRG ++F+IQ T P N+ Sbjct: 8 VFSGNANLPLAEGVVRKLNMRLGMATVGRFSDGEIAVEIEEHVRGREVFVIQPTSQPINE 67 Query: 66 NLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVRSARVPITAKVVADFLSSVGVDRV 125 NLMEL+VM+DALRR+SA ITAVIPYFGYARQDRR+RSARVPITAK+VA + + G DRV Sbjct: 68 NLMELLVMIDALRRSSASVITAVIPYFGYARQDRRIRSARVPITAKLVAKMICAAGADRV 127 Query: 126 LTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLNLDNPIVVSPDIGGVVRARAIAKLLN 185 LTVDLHA+QIQGFFDVPVDNV+ SPILL D+ + + +VVSPD+GGVVRARA+AK L+ Sbjct: 128 LTVDLHADQIQGFFDVPVDNVYASPILLGDVWRQKYPDLMVVSPDVGGVVRARALAKRLD 187 Query: 186 DTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVLVDDMIDTGGTLCKAAEALKERGAKRVF 245 D D+AIIDKRRPRAN ++VM+IIGDV GR CV+VDD++DT GTLCKAA ALKE GA+RV Sbjct: 188 DADLAIIDKRRPRANEAKVMNIIGDVRGRTCVMVDDLVDTAGTLCKAAGALKEHGAERVV 247 Query: 246 AYATHPIFSGNAANNLRNSVIDEVVVCDTIPLTDEIKALPNVRTLTLSGMLAEAIRRISN 305 AY THP+ SG A +N+ S++DE+VV DTIPL + A +R LT++ MLAE IRRI+ Sbjct: 248 AYCTHPVLSGPAVDNIEASMLDELVVTDTIPLQERASACNRIRQLTIAEMLAETIRRIAM 307 Query: 306 EESISAMF 313 ES+S+++ Sbjct: 308 GESVSSLY 315 Lambda K H 0.322 0.137 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 334 Number of extensions: 9 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 315 Length of database: 317 Length adjustment: 27 Effective length of query: 288 Effective length of database: 290 Effective search space: 83520 Effective search space used: 83520 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 48 (23.1 bits)
Align candidate WP_017364368.1 MCA_RS05175 (ribose-phosphate diphosphokinase)
to HMM TIGR01251 (prs: ribose-phosphate diphosphokinase (EC 2.7.6.1))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR01251.hmm # target sequence database: /tmp/gapView.28250.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR01251 [M=309] Accession: TIGR01251 Description: ribP_PPkin: ribose-phosphate diphosphokinase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 2e-121 390.6 0.2 2.3e-121 390.4 0.2 1.0 1 lcl|NCBI__GCF_000008325.1:WP_017364368.1 MCA_RS05175 ribose-phosphate dip Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_000008325.1:WP_017364368.1 MCA_RS05175 ribose-phosphate diphosphokinase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 390.4 0.2 2.3e-121 2.3e-121 2 308 .. 7 315 .. 6 316 .. 0.98 Alignments for each domain: == domain 1 score: 390.4 bits; conditional E-value: 2.3e-121 TIGR01251 2 kilsgssskelaekvaknlglelgdvevkkFadgElyvrieesvrgkdvfiivqstsapvndalmelll 70 +++sg+++ lae v ++l+++lg ++v +F+dgE+ v+iee+vrg++vf+i q ts+p+n++lmell+ lcl|NCBI__GCF_000008325.1:WP_017364368.1 7 MVFSGNANLPLAEGVVRKLNMRLGMATVGRFSDGEIAVEIEEHVRGREVFVI-QPTSQPINENLMELLV 74 79**************************************************.**************** PP TIGR01251 71 lidalkrasaksvtaviPyygYaRqdkkak.srepisaklvaklleeaGadrvltvdlHseqiqgfFdv 138 +idal+r+sa+ +taviPy+gYaRqd++ + r pi+aklvak++ +aGadrvltvdlH++qiqgfFdv lcl|NCBI__GCF_000008325.1:WP_017364368.1 75 MIDALRRSSASVITAVIPYFGYARQDRRIRsARVPITAKLVAKMICAAGADRVLTVDLHADQIQGFFDV 143 ****************************9857************************************* PP TIGR01251 139 pvenlsaspklieelkkkelknlvvvsPDkGaverakkvakklg.lelaiieKeRdskenevevtnllg 206 pv+n++asp l+ ++ +++ +l+vvsPD G+v ra+++ak+l+ ++laii+K+R+ ++ne++v+n++g lcl|NCBI__GCF_000008325.1:WP_017364368.1 144 PVDNVYASPILLGDVWRQKYPDLMVVSPDVGGVVRARALAKRLDdADLAIIDKRRP-RANEAKVMNIIG 211 ********************************************9***********.889********* PP TIGR01251 207 dvegkdvvivDDiisTggTlvkaaelLkekGAkkvivaathgvfsgdAlerlaeagveevivtntilve 275 dv g+++v+vDD+++T+gTl+kaa +Lke+GA++v++++th+v+sg+A+ +++ ++++e++vt+ti+ + lcl|NCBI__GCF_000008325.1:WP_017364368.1 212 DVRGRTCVMVDDLVDTAGTLCKAAGALKEHGAERVVAYCTHPVLSGPAVDNIEASMLDELVVTDTIPLQ 280 ********************************************************************5 PP TIGR01251 276 e..kklpkvseisvapliaeaiarihenesvsslf 308 e +++++++++++a+++ae+i+ri+ +esvssl+ lcl|NCBI__GCF_000008325.1:WP_017364368.1 281 EraSACNRIRQLTIAEMLAETIRRIAMGESVSSLY 315 55899****************************98 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (309 nodes) Target sequences: 1 (317 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01 # Mc/sec: 7.63 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory