Alignments for a candidate for hisC in Nocardiopsis lucentensis DSM 44048

GapMind for Amino acid biosynthesis

Alignments for a candidate for hisC in Nocardiopsis lucentensis DSM 44048

Align Histidinol-phosphate aminotransferase 1; EC 2.6.1.9; Imidazole acetol-phosphate transaminase 1 (uncharacterized)
to candidate WP_017599139.1 D471_RS0113730 histidinol-phosphate transaminase

Query= curated2:Q46Y48
         (376 letters)



>NCBI__GCF_000341125.1:WP_017599139.1
          Length = 360

 Score =  233 bits (594), Expect = 6e-66
 Identities = 138/326 (42%), Positives = 183/326 (56%), Gaps = 9/326 (2%)

Query: 45  VKLASNENPLGMPESAKHAAAAAIAELGRYPDSNGFELKAALSTKLGVPQDWLTLGNGSN 104
           +KL+SNE+P G   S + A A A AEL RYPD     L   L+  LGVP++ + LG GS 
Sbjct: 34  IKLSSNESPFGPLPSVREAVAQASAELNRYPDPGATALVTELARHLGVPEEHVALGAGSV 93

Query: 105 DILELAAHALVTPGQSIVYAEYSFAVYALATQEIGARAIVVKARDYGHDLDAMAAAITSD 164
            +L+    A+  PG  +VYA  SF  Y L  +  G  ++ V  RD  HDLDA+A AIT  
Sbjct: 94  GLLQQLLEAVGEPGAEVVYAWRSFEAYPLLAELAGVTSVRVPLRDETHDLDAIADAITES 153

Query: 165 TRLVFIANPNNPTGTFVPAAALETFLAKVPAEVVVVLDEAYNEYLDDDQQYDSVAWVRRY 224
           TR+V + NPNNPTGT VP   L  FL +VP  V+V+LDEAY+EY+ D +  D V   R  
Sbjct: 154 TRMVLVCNPNNPTGTTVPKGELLAFLDRVPEHVLVILDEAYHEYVRDPRVPDGVELYRDR 213

Query: 225 PNLLVSRTFSKAYGLAGLRIGYAVAQPELTDLLNRIRQPFNVNSVAQAAAVAALGDTAFL 284
           PN+ V RTFSKAYGLA +R+GY VA P +     +   PF VN +AQAA +A+L     L
Sbjct: 214 PNVAVLRTFSKAYGLAAVRLGYLVAHPHVAAAARKTLVPFAVNHLAQAAGIASLAARDEL 273

Query: 285 QRSAELNRAGKAQLVEAFSRLGLEFVASSGNFVLVRVGDDD-DAGARVNVALLRQGVIVR 343
               E     + ++ +A    G     +  NFV +RVG+D  D  A       + GV VR
Sbjct: 274 MERVEATVGERTRVRDALIASGWTVPPTEANFVWLRVGEDTLDFAAE----CAKVGVSVR 329

Query: 344 PVGNYGMPRWLRVTIGLPDENAAFIA 369
           P    G     RV++G P+EN  F++
Sbjct: 330 PFEGEG----ARVSLGTPEENDVFLS 351


Lambda     K      H
   0.318    0.134    0.382 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 336
Number of extensions: 9
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 376
Length of database: 360
Length adjustment: 30
Effective length of query: 346
Effective length of database: 330
Effective search space:   114180
Effective search space used:   114180
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 49 (23.5 bits)

Align candidate WP_017599139.1 D471_RS0113730 (histidinol-phosphate transaminase)
to HMM TIGR01141 (hisC: histidinol-phosphate transaminase (EC 2.6.1.9))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR01141.hmm
# target sequence database:        /tmp/gapView.16166.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01141  [M=349]
Accession:   TIGR01141
Description: hisC: histidinol-phosphate transaminase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
    2.6e-91  292.1   0.0    2.9e-91  291.9   0.0    1.0  1  lcl|NCBI__GCF_000341125.1:WP_017599139.1  D471_RS0113730 histidinol-phosph


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000341125.1:WP_017599139.1  D471_RS0113730 histidinol-phosphate transaminase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  291.9   0.0   2.9e-91   2.9e-91       2     345 ..      12     352 ..      11     356 .. 0.97

  Alignments for each domain:
  == domain 1  score: 291.9 bits;  conditional E-value: 2.9e-91
                                 TIGR01141   2 ekikklepYqpgarelgek.evvkLnsnEnPfgpsekvkealkeelkklhrYpdpqalelkealakylg 69 
                                                 ++++ +Y+pg + +g +   +kL+snE+Pfgp ++v+ea+ ++ ++l+rYpdp a++l ++la++lg
  lcl|NCBI__GCF_000341125.1:WP_017599139.1  12 SVLESIPAYKPGRKVVGPDgRSIKLSSNESPFGPLPSVREAVAQASAELNRYPDPGATALVTELARHLG 80 
                                               5688999*****888888899************************99********************** PP

                                 TIGR01141  70 veeenillgnGsdelielliraflepgdavlvleptysmYevsakiagaevkevplkedgqedleavle 138
                                               v ee ++lg+Gs +l++ l++a+ epg++v+++  +++ Y++ a++ag+  + vpl+++ ++dl+a+++
  lcl|NCBI__GCF_000341125.1:WP_017599139.1  81 VPEEHVALGAGSVGLLQQLLEAVGEPGAEVVYAWRSFEAYPLLAELAGVTSVRVPLRDE-THDLDAIAD 148
                                               **********************************************************9.6******** PP

                                 TIGR01141 139 aakekvklvflasPnnPtGnllkreeiekvleev.edalVVvDeAYieFsee...asvlellaeypnlv 203
                                               a++e++++v +++PnnPtG+ + + e+ ++l++v e++lV++DeAY e+ ++   ++ +el +++pn++
  lcl|NCBI__GCF_000341125.1:WP_017599139.1 149 AITESTRMVLVCNPNNPTGTTVPKGELLAFLDRVpEHVLVILDEAYHEYVRDprvPDGVELYRDRPNVA 217
                                               **********************************99****************9999************* PP

                                 TIGR01141 204 vlrTlSKafgLAglRvGyaianaeiiealekvrapynvsslaleaavaalrdsdkiektveevkkerer 272
                                               vlrT+SKa+gLA++R+Gy++a+++++ a +k   p+ v+ la++a++a+l  +d+++++ve+   er+r
  lcl|NCBI__GCF_000341125.1:WP_017599139.1 218 VLRTFSKAYGLAAVRLGYLVAHPHVAAAARKTLVPFAVNHLAQAAGIASLAARDELMERVEATVGERTR 286
                                               ********************************************************************* PP

                                 TIGR01141 273 lleelkkleglevyeSkaNFvlikvkedaeelleallekgiivRdlksaeglleeclRitvGtreener 341
                                               + ++l +  g  v + +aNFv ++v ed  +++ +  + g+ vR ++   g      R++ Gt+een+ 
  lcl|NCBI__GCF_000341125.1:WP_017599139.1 287 VRDALIAS-GWTVPPTEANFVWLRVGEDTLDFAAECAKVGVSVRPFEGE-G-----ARVSLGTPEENDV 348
                                               ******98.89***********************************966.6.....5************ PP

                                 TIGR01141 342 llea 345
                                               +l +
  lcl|NCBI__GCF_000341125.1:WP_017599139.1 349 FLSV 352
                                               *987 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (349 nodes)
Target sequences:                          1  (360 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01
# Mc/sec: 9.79
//
[ok]

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis