Alignments for a candidate for ilvI in Nocardiopsis lucentensis DSM 44048

GapMind for Amino acid biosynthesis

Alignments for a candidate for ilvI in Nocardiopsis lucentensis DSM 44048

Align Probable acetolactate synthase large subunit; AHAS; EC 2.2.1.6; Acetohydroxy-acid synthase large subunit; ALS (uncharacterized)
to candidate WP_017599575.1 D471_RS0116105 glyoxylate carboligase

Query= curated2:O08353
         (599 letters)



>NCBI__GCF_000341125.1:WP_017599575.1
          Length = 571

 Score =  387 bits (995), Expect = e-112
 Identities = 218/586 (37%), Positives = 333/586 (56%), Gaps = 36/586 (6%)

Query: 1   MNGAEAMIKALEAEKVEILFGYPGGALLPFYDALHH-SDLIHLLTRHEQAAAHAADGYAR 59
           M    A+I+ L+ E V+  FG PG A+LP Y A+     + HL  RHE+ A H ADG++R
Sbjct: 4   MPAMNAVIEVLKDEGVDTAFGCPGAAILPLYKAMEDVGGIEHLTVRHEEGATHMADGWSR 63

Query: 60  ASGKVGVCIGTSGPGATNLVTGVATAHSDSSPMVALTGQVPTKLIGNDAFQEIDALGLFM 119
            +GKVGV IGTSGP  TN++TG+ TA +DS P+V +TGQ  T L+  + FQ +D + +  
Sbjct: 64  TTGKVGVAIGTSGPAGTNMITGLYTAMADSVPIVCITGQAMTSLLDKEGFQAVDIVEIAK 123

Query: 120 PIVKHNFQIQKTCQIPEIFRSAFEIAQTGRPGPVHIDLPKDVQELELDIDKHPIPSKVKL 179
           P+ K   QI++    P IFR AF +AQ GRPGPV +D+P DV +  ++ D   I + +++
Sbjct: 124 PVTKWAVQIREAATAPWIFREAFRVAQEGRPGPVLVDIPLDVAKQVIEYDP-AIDAPLRI 182

Query: 180 IGYNPTTIGHPRQIKKAIKLIASAKRPIILAGGGVLLSGANEELLKLVELLNIPVCTTLM 239
               P    H  ++++A+ ++ +A+RP++LAGGGV+LS A+EEL +L E LN+PV  TLM
Sbjct: 183 NSVEP----HQPRVERALDMLLAAERPLLLAGGGVILSEASEELKELAEYLNVPVQVTLM 238

Query: 240 GKGCISENHPLALGMVGMH-GTKPANYCLSESDVLISIGCRFSDRITGDIKSFATNAKII 298
           GKG   E+ PL  GM G+    +  N    ESD+++++G RF DR TG I  +  +   I
Sbjct: 239 GKGSFDEDSPLYSGMTGVQTSQRYGNASFLESDLVLAVGARFGDRHTGQIDVYRGDRTFI 298

Query: 299 HIDIDPAEIGKNVNVDVPIVGDAKLILKEVIKQLDYIINKDSKENNDKENISQWIENVNS 358
           H+DI+P +IG+    D+ +V DAKL L  +++         +K   +K  +  W++ V  
Sbjct: 299 HVDIEPTQIGRVFEPDLGVVSDAKLFLSALLEA--------AKARGEKAEVQPWVDRVGE 350

Query: 359 LKKSSIPVMDYDDIPIKPQKIVKELMAVIDDLNINKNTIITTDVGQNQMWMAHYFKTQTP 418
           LK++     D+D +PIK  ++ KE+  V D      +T   T +G  Q+W   + K   P
Sbjct: 351 LKRTMTRREDFDSVPIKAPRVYKEINEVFD-----ADTYFVTAIGLYQIWGGQHQKAYKP 405

Query: 419 RSFLSSGGLGTMGFGFPSAIGAKVA----KPDSKVICITGDGGFMMNCQELGTIAEYNIP 474
           R +   G  G +G+  P+AIG K A    +PD++V+ I GD GF    +EL   A+YN+P
Sbjct: 406 RHYQICGQAGPLGWEIPAAIGVKKALKNTEPDAEVVGIVGDYGFQYMVEELAVAAQYNVP 465

Query: 475 VVICIFDNRTLGMVYQWQNLFYGKRQCSVNFGG-APDFIKLAESYGIKARRIESPNEINE 533
            VI + +N  LG++ Q    +    Q  +++     D +K+ E+YG   RR+  PN+I E
Sbjct: 466 YVIIMINNEYLGLIRQASIPYEMNYQVDIHYDEFGTDNVKIMEAYGCSGRRVADPNDIRE 525

Query: 534 AL----KEAINCDEPYLLDFAIDPSSALSMVPPGAKLTNIIDAVQE 575
           +L    KEA     P L++  I+  +     P        IDAV+E
Sbjct: 526 SLEWARKEAKATSRPVLVEIMIEREANTPHGPS-------IDAVKE 564


Lambda     K      H
   0.319    0.137    0.405 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 816
Number of extensions: 43
Number of successful extensions: 8
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 599
Length of database: 571
Length adjustment: 37
Effective length of query: 562
Effective length of database: 534
Effective search space:   300108
Effective search space used:   300108
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis