Align acetohydroxyacid isomeroreductase (EC 1.1.1.86) (characterized)
to candidate WP_018232000.1 THITHI_RS0105105 ketol-acid reductoisomerase
Query= metacyc::MONOMER-18814 (338 letters) >NCBI__GCF_000378965.1:WP_018232000.1 Length = 338 Score = 489 bits (1259), Expect = e-143 Identities = 239/338 (70%), Positives = 284/338 (84%) Query: 1 MKVFYDKDADLSLIKGKNVTIIGYGSQGHAHALNLKDSGVNVTVGLRKSGASWNKAANAG 60 M+V+YDKDADLS+I+G V I+GYGSQGHAHA NLKDSGV+VTV LR AS KA AG Sbjct: 1 MQVYYDKDADLSIIQGMKVAIVGYGSQGHAHANNLKDSGVDVTVALRAGSASARKAEGAG 60 Query: 61 LQVKEVAEAVKGADVVMILLPDEQIADVYKNEVHDNIKEGAALAFAHGFNVHYGAVIPRA 120 L+V+ + +AVKGAD+VMIL PDE A +Y+++V N+K+G LAFAHGFN+H+ + PR+ Sbjct: 61 LKVQGIEDAVKGADLVMILAPDEHQAALYQDQVGPNLKQGGVLAFAHGFNIHFEQIEPRS 120 Query: 121 DLDVIMIAPKAPGHTVRATYTQGGGVPHLIAVHQNKSGAARDIALSYATANGGGRAGIIE 180 DLDVIM+APK PGH VR+TYTQGGGVP LIAV+Q+ SG ARDIALSYA+ANGGGRAGIIE Sbjct: 121 DLDVIMVAPKGPGHLVRSTYTQGGGVPSLIAVYQDASGRARDIALSYASANGGGRAGIIE 180 Query: 181 TNFREETETDLFGEQAVLCGGTVELIKAGFETLVEAGYAPEMAYFECLHELKLIVDLIYE 240 T+F++ETETDLFGEQAVLCGG L++AGFETLVEAGY PEMAYFECLHELKLIVDL+YE Sbjct: 181 TSFKDETETDLFGEQAVLCGGATALVQAGFETLVEAGYPPEMAYFECLHELKLIVDLMYE 240 Query: 241 GGIANMNYSISNNAEYGEYVTGPRVVTEETKKAMKQCLTDIQTGEYAKSFLLENKAGAPT 300 GGIA+M YSISN AEYG+ GPRVVTE+TK MK+ LT+IQTG +A+ F+LENKAGA T Sbjct: 241 GGIADMRYSISNTAEYGDITRGPRVVTEQTKAEMKKILTEIQTGRFAREFILENKAGAAT 300 Query: 301 LISRRRLTAEHQIEEVGAKLRAMMPWIAKNKMVDQSKN 338 L + RR+ EH IE VG++LR MMPWI NKMVD+SKN Sbjct: 301 LKASRRIAREHPIEVVGSRLRDMMPWIKANKMVDKSKN 338 Lambda K H 0.316 0.133 0.379 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 459 Number of extensions: 12 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 338 Length of database: 338 Length adjustment: 28 Effective length of query: 310 Effective length of database: 310 Effective search space: 96100 Effective search space used: 96100 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 49 (23.5 bits)
Align candidate WP_018232000.1 THITHI_RS0105105 (ketol-acid reductoisomerase)
to HMM TIGR00465 (ilvC: ketol-acid reductoisomerase (EC 1.1.1.86))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00465.hmm # target sequence database: /tmp/gapView.13064.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00465 [M=314] Accession: TIGR00465 Description: ilvC: ketol-acid reductoisomerase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 1.5e-140 453.6 0.4 1.7e-140 453.4 0.4 1.0 1 lcl|NCBI__GCF_000378965.1:WP_018232000.1 THITHI_RS0105105 ketol-acid redu Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_000378965.1:WP_018232000.1 THITHI_RS0105105 ketol-acid reductoisomerase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 453.4 0.4 1.7e-140 1.7e-140 1 313 [. 14 327 .. 14 328 .. 0.99 Alignments for each domain: == domain 1 score: 453.4 bits; conditional E-value: 1.7e-140 TIGR00465 1 lkgkkvaiiGyGsqGeaqalnlrdsglnvivglrkeaaswkkAeedGfkvltveeaikkadlimiLlpD 69 ++g kvai+GyGsqG+a+a nl+dsg++v+v+lr ++as +kAe G+kv +e+a+k adl+miL pD lcl|NCBI__GCF_000378965.1:WP_018232000.1 14 IQGMKVAIVGYGSQGHAHANNLKDSGVDVTVALRAGSASARKAEGAGLKVQGIEDAVKGADLVMILAPD 82 6899***************************************************************** PP TIGR00465 70 evqkevyeaeikpllkegkallfsHGfnivfkqivipkdvdvvlvAPKgpGalvReeykegrGvpsliA 138 e q + y+ ++ p+lk+g +l f+HGfni+f+qi+++ d+dv++vAPKgpG+lvR++y +g GvpsliA lcl|NCBI__GCF_000378965.1:WP_018232000.1 83 EHQAALYQDQVGPNLKQGGVLAFAHGFNIHFEQIEPRSDLDVIMVAPKGPGHLVRSTYTQGGGVPSLIA 151 ********************************************************************* PP TIGR00465 139 veqdvtgeakeiAlayAkaiGgaragvlettFkeEvesDLfGEqavLcGglealikaafdtLveaGyqp 207 v+qd++g a++iAl+yA a Gg+rag++et+Fk+E+e+DLfGEqavLcGg +al++a+f+tLveaGy p lcl|NCBI__GCF_000378965.1:WP_018232000.1 152 VYQDASGRARDIALSYASANGGGRAGIIETSFKDETETDLFGEQAVLCGGATALVQAGFETLVEAGYPP 220 ********************************************************************* PP TIGR00465 208 elAyfeivhelklivdllkekGlelmrdavsntAklgalelr.eilkeelkkemqkilkeiqnGefake 275 e+Ayfe++helklivdl++e+G++ mr ++sntA++g+++++ ++++e++k+em+kil eiq+G+fa+e lcl|NCBI__GCF_000378965.1:WP_018232000.1 221 EMAYFECLHELKLIVDLMYEGGIADMRYSISNTAEYGDITRGpRVVTEQTKAEMKKILTEIQTGRFARE 289 ********************************************************************* PP TIGR00465 276 walekeagkpafeearkkekeqeiekvGkelralvkae 313 ++le++ag++++++ r+ +e+ ie vG++lr+++++ lcl|NCBI__GCF_000378965.1:WP_018232000.1 290 FILENKAGAATLKASRRIAREHPIEVVGSRLRDMMPWI 327 ***********************************986 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (314 nodes) Target sequences: 1 (338 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01 # Mc/sec: 9.70 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory