Align Aspartate--tRNA(Asp/Asn) ligase; Aspartyl-tRNA synthetase; AspRS; Non-discriminating aspartyl-tRNA synthetase; ND-AspRS; EC 6.1.1.23 (characterized)
to candidate WP_019865998.1 METMI_RS0109240 aspartate--tRNA ligase
Query= SwissProt::Q51422 (591 letters) >NCBI__GCF_000384075.1:WP_019865998.1 Length = 595 Score = 781 bits (2016), Expect = 0.0 Identities = 384/586 (65%), Positives = 467/586 (79%), Gaps = 3/586 (0%) Query: 2 MRSHYCGQLNESLDGQEVTLCGWVHRRRDHGGVIFLDVRDREGLAQVVFDPDRAETFAKA 61 MR+H CG+L+ G+ VT+CGWVHRRRDHGGVIF+D+RDR GL QVV DPD FA A Sbjct: 1 MRTHKCGELDTQQLGETVTICGWVHRRRDHGGVIFIDLRDRAGLVQVVIDPDTEAAFAAA 60 Query: 62 DRVRSEFVVKITGKVRLRPEGARNPNMASGSIEVLGYELEVLNQAETPPFPLDEYSDVGE 121 + VRSE+V+KI+G VR RP G N NM SG+IE+L E+EVLN++ETPPFP++ +V E Sbjct: 61 EHVRSEYVLKISGIVRHRPGGTVNANMRSGAIEILAKEIEVLNESETPPFPIESEIEVNE 120 Query: 122 ETRLRYRFIDLRRPEMAAKLKLRARITSSIRRYLDDNGFLDVETPILGRPTPEGARDYLV 181 E RLRYR+IDLRR M K+K+R +T +R +LDDN F ++ETP L + TPEGARDY+V Sbjct: 121 ELRLRYRYIDLRRVAMQEKMKVRRDVTRVLRNFLDDNEFFEIETPYLTKATPEGARDYIV 180 Query: 182 PSRTYPGHFFALPQSPQLFKQLLMVAGFDRYYQIAKCFRDEDLRADRQPEFTQIDIETSF 241 PSRT+ FFALPQSPQL+KQ+LMVAG DRYYQ+ +CFRDEDLRADRQPEFTQ+DIETSF Sbjct: 181 PSRTHVNSFFALPQSPQLYKQILMVAGMDRYYQVVRCFRDEDLRADRQPEFTQLDIETSF 240 Query: 242 LDESDIIGITEKMVRQLFKEVLDVEFD-EFPHMPFEEAMRRYGSDKPDLRIPLELVDVAD 300 + E I+ I E+M+RQLF +V++V EFP M ++EAM R+G D+PDLRIPLELVDVA+ Sbjct: 241 MTEDQIMAIMEEMIRQLFAKVINVALPAEFPRMTYQEAMSRFGIDRPDLRIPLELVDVAE 300 Query: 301 QLKEVEFKVFSGPANDPKGRVAALRVPGAA-SMPRSQIDDYTKFVGIYGAKGLAYIKVNE 359 LK+VEFKVFSGPANDPKGRV A+RVPGA + R ID+ TK+VGIYGAKGLAYIKVN+ Sbjct: 301 DLKDVEFKVFSGPANDPKGRVVAMRVPGAGEKLSRKDIDELTKYVGIYGAKGLAYIKVND 360 Query: 360 RAKGVEGLQSPIVKFIPEANLNVILDRVGAVDGDIVFFGADKAKIVCDALGALRIKVGHD 419 A G+EGLQSPIVKF P I+ + GA +GD++FFGADK IV +A+GALR+K+GHD Sbjct: 361 LAAGLEGLQSPIVKFAPAEAWASIMAKTGAQNGDLLFFGADKTSIVNEAMGALRVKLGHD 420 Query: 420 LKLLTREWAPMWVVDFPMFE-ENDDGSLSALHHPFTSPKCTPAELEANPGAALSRAYDMV 478 L L+ EW P+WVVDFPMF+ + G +A+HHPFT+P C+ +L ANPGAALSRAYD+V Sbjct: 421 LNLIEGEWQPVWVVDFPMFDWDEKSGRYNAIHHPFTAPSCSVEDLVANPGAALSRAYDLV 480 Query: 479 LNGTELGGGSIRIHDKSMQQAVFRVLGIDEAEQEEKFGFLLDALKYGAPPHGGLAFGLDR 538 LNGTE+GGGSIRI+ +MQQ VF +LGI E E +EKFGFLLDALKYGAPPHGGLAFGLDR Sbjct: 481 LNGTEVGGGSIRINRPAMQQTVFGILGIGEEEAQEKFGFLLDALKYGAPPHGGLAFGLDR 540 Query: 539 LVMLMTGASSIREVIAFPKTQSAGDVMTQAPGSVDGKALRELHIRL 584 LVMLMTG+ SIR+VIAFPKTQSA + AP V + L+EL IRL Sbjct: 541 LVMLMTGSHSIRDVIAFPKTQSAACPLVSAPAVVSDEQLKELGIRL 586 Lambda K H 0.321 0.140 0.411 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1081 Number of extensions: 37 Number of successful extensions: 4 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 591 Length of database: 595 Length adjustment: 37 Effective length of query: 554 Effective length of database: 558 Effective search space: 309132 Effective search space used: 309132 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 53 (25.0 bits)
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory