Alignments for a candidate for lysA in Methylovulum miyakonense HT12

GapMind for Amino acid biosynthesis

Alignments for a candidate for lysA in Methylovulum miyakonense HT12

Align diaminopimelate decarboxylase (EC 4.1.1.20) (characterized)
to candidate WP_019866655.1 METMI_RS0112590 diaminopimelate decarboxylase

Query= BRENDA::Q9KVL7
         (417 letters)



>NCBI__GCF_000384075.1:WP_019866655.1
          Length = 415

 Score =  559 bits (1440), Expect = e-164
 Identities = 271/417 (64%), Positives = 339/417 (81%), Gaps = 3/417 (0%)

Query: 1   MDYFNYQEDGQLWAEQVPLADLANQYGTPLYVYSRATLERHWHAFDKSVGDYPHLICYAV 60
           MDYFNY+ D +L+AE V + D+  ++G+P Y+YSRATLERHW AFD + G+ PHL+CYAV
Sbjct: 1   MDYFNYR-DNELFAEDVAVQDIVYKFGSPCYIYSRATLERHWKAFDGAFGNTPHLVCYAV 59

Query: 61  KANSNLGVLNTLARLGSGFDIVSVGELERVLAAGGDPSKVVFSGVGKTEAEMKRALQLKI 120
           KANSNLG+LN LARLGSGFDIVS+GE+ERVLAAGGD  K+VFSGVGK E E+  AL+  I
Sbjct: 60  KANSNLGLLNVLARLGSGFDIVSLGEMERVLAAGGDAKKIVFSGVGKREDEILAALKTGI 119

Query: 121 KCFNVESEPELQRLNKVAGELGVKAPISLRINPDVDAKTHPYISTGLRDNKFGITFDRAA 180
           +CFN+E   EL R+N +AG+LGV AP+S R+NPDVDAKTHPYISTGL++NKFGI  D A 
Sbjct: 120 RCFNIEVSGELDRINTLAGQLGVAAPVSFRVNPDVDAKTHPYISTGLKENKFGIDIDTAI 179

Query: 181 QVYRLAHSLPNLDVHGIDCHIGSQLTALAPFIDATDRLLALIDSLKAEGIHIRHLDVGGG 240
             YR A ++PN++V GIDCHIGSQLT   PF+DA D+LL L+ +L+ EGI ++HLD+GGG
Sbjct: 180 AEYRRAAAMPNINVVGIDCHIGSQLTETRPFLDALDKLLELVANLQKEGIRLQHLDLGGG 239

Query: 241 LGVVYRDELPPQPSEYAKALLDRLERHRDLELIFEPGRAIAANAGVLVTKVEFLKHTEHK 300
           LG+ Y+DE PP+PSEY +A+L RL +H D E++ EPGRAI  NAG+LVT+VE+LK T +K
Sbjct: 240 LGICYKDEQPPEPSEYIQAVLARLGQH-DYEILLEPGRAIVGNAGILVTQVEYLKPTANK 298

Query: 301 NFAIIDAAMNDLIRPALYQAWQDIIPLRPRQGEAQ-TYDLVGPVCETSDFLGKDRDLVLQ 359
           NFAI+DAAMNDL+RP+LY AWQ+IIP+ P     +  +D+VGPVCET DFLGK R L L 
Sbjct: 299 NFAIVDAAMNDLLRPSLYSAWQNIIPVSPHDDAPEIQWDIVGPVCETGDFLGKGRVLKLA 358

Query: 360 EGDLLAVRSSGAYGFTMSSNYNTRPRVAEVMVDGNKTYLVRQREELSSLWALESVLP 416
           +GDLLA+RS+GAYGF+MSSNYN+RPRVAEVMVDG + +LVR RE L+ LWA E +LP
Sbjct: 359 QGDLLAIRSAGAYGFSMSSNYNSRPRVAEVMVDGGQCHLVRARETLAQLWAGERLLP 415


Lambda     K      H
   0.319    0.137    0.405 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 541
Number of extensions: 17
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 417
Length of database: 415
Length adjustment: 31
Effective length of query: 386
Effective length of database: 384
Effective search space:   148224
Effective search space used:   148224
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 50 (23.9 bits)

Align candidate WP_019866655.1 METMI_RS0112590 (diaminopimelate decarboxylase)
to HMM TIGR01048 (lysA: diaminopimelate decarboxylase (EC 4.1.1.20))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR01048.hmm
# target sequence database:        /tmp/gapView.576.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01048  [M=417]
Accession:   TIGR01048
Description: lysA: diaminopimelate decarboxylase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
   4.2e-165  535.2   0.0   5.1e-165  534.9   0.0    1.0  1  lcl|NCBI__GCF_000384075.1:WP_019866655.1  METMI_RS0112590 diaminopimelate 


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000384075.1:WP_019866655.1  METMI_RS0112590 diaminopimelate decarboxylase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  534.9   0.0  5.1e-165  5.1e-165       5     416 ..       7     411 ..       4     412 .. 0.99

  Alignments for each domain:
  == domain 1  score: 534.9 bits;  conditional E-value: 5.1e-165
                                 TIGR01048   5 kdgeleiegvdlkelaeefgtPlYvydeetlrerlealkeafkaeeslvlYAvKAnsnlavlrllaeeG 73 
                                               +d el++e+v++++++ +fg+P Y+y+++tl+++++a++ af+++ +lv+YAvKAnsnl +l++la++G
  lcl|NCBI__GCF_000384075.1:WP_019866655.1   7 RDNELFAEDVAVQDIVYKFGSPCYIYSRATLERHWKAFDGAFGNTPHLVCYAVKANSNLGLLNVLARLG 75 
                                               67899**************************************************************** PP

                                 TIGR01048  74 lgldvvsgGEleralaAgvkaekivfsgngkseeeleaaleleiklinvdsveelelleeiakelgkka 142
                                               +g+d+vs GE+er+laAg +a+kivfsg+gk+e+e+ aal+++i+++n++   el++++++a++lg  a
  lcl|NCBI__GCF_000384075.1:WP_019866655.1  76 SGFDIVSLGEMERVLAAGGDAKKIVFSGVGKREDEILAALKTGIRCFNIEVSGELDRINTLAGQLGVAA 144
                                               ********************************************************************* PP

                                 TIGR01048 143 rvllRvnpdvdaktheyisTGlkesKFGieveeaeeayelalkleslelvGihvHIGSqildlepfvea 211
                                               +v++Rvnpdvdakth+yisTGlke+KFGi++++a + y++a++++++++vGi++HIGSq++++ pf +a
  lcl|NCBI__GCF_000384075.1:WP_019866655.1 145 PVSFRVNPDVDAKTHPYISTGLKENKFGIDIDTAIAEYRRAAAMPNINVVGIDCHIGSQLTETRPFLDA 213
                                               ********************************************************************* PP

                                 TIGR01048 212 aekvvklleelkeegieleeldlGGGlgisyeeeeeapdleeyaeklleklekeaelglklklilEpGR 280
                                               ++k+++l+++l++egi+l++ldlGGGlgi+y++e+ +p+++ey +++l++l +     ++ +++lEpGR
  lcl|NCBI__GCF_000384075.1:WP_019866655.1 214 LDKLLELVANLQKEGIRLQHLDLGGGLGICYKDEQ-PPEPSEYIQAVLARLGQ-----HDYEILLEPGR 276
                                               ********************************999.**************999.....7********** PP

                                 TIGR01048 281 slvanagvlltrVesvKevesrkfvlvDagmndliRpalYeayheiaalkrleeeetetvdvvGplCEs 349
                                               ++v+nag+l+t+Ve++K + +++f++vDa+mndl+Rp+lY+a+++i++++  +++++ ++d+vGp+CE+
  lcl|NCBI__GCF_000384075.1:WP_019866655.1 277 AIVGNAGILVTQVEYLKPTANKNFAIVDAAMNDLLRPSLYSAWQNIIPVSPHDDAPEIQWDIVGPVCET 345
                                               ********************************************************************* PP

                                 TIGR01048 350 gDvlakdrelpeveeGdllavasaGAYgasmssnYnsrprpaevlveegkarlirrretledllale 416
                                               gD+l+k r l+ ++ Gdlla++saGAYg+smssnYnsrpr+aev+v++g+++l+r retl++l+a e
  lcl|NCBI__GCF_000384075.1:WP_019866655.1 346 GDFLGKGRVLKLAQ-GDLLAIRSAGAYGFSMSSNYNSRPRVAEVMVDGGQCHLVRARETLAQLWAGE 411
                                               **********9998.*************************************************976 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (417 nodes)
Target sequences:                          1  (415 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.02u 0.01s 00:00:00.03 Elapsed: 00:00:00.02
# Mc/sec: 6.67
//
[ok]

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis