Alignments for a candidate for proA in Hydrogenovibrio halophilus DSM 15072

GapMind for Amino acid biosynthesis

Alignments for a candidate for proA in Hydrogenovibrio halophilus DSM 15072

Align Gamma-glutamyl phosphate reductase; GPR; Glutamate-5-semialdehyde dehydrogenase; Glutamyl-gamma-semialdehyde dehydrogenase; GSA dehydrogenase; EC 1.2.1.41 (characterized)
to candidate WP_019895556.1 A377_RS0107085 glutamate-5-semialdehyde dehydrogenase

Query= SwissProt::P07004
         (417 letters)



>NCBI__GCF_000384235.1:WP_019895556.1
          Length = 422

 Score =  363 bits (931), Expect = e-105
 Identities = 185/416 (44%), Positives = 276/416 (66%), Gaps = 4/416 (0%)

Query: 1   MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILNANAQDVADARANGLSE 60
           ++ Q+G AA+ A+ +L+   + +KN  LE IA E+E ++E + + NA+D+   +ANGL  
Sbjct: 10  LMHQLGQAARTAATQLSVADTAQKNAALEAIAVEIERRAEFLQSENARDLDAGKANGLDA 69

Query: 61  AMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSGLRLERRRVPLGVIGVIYE 120
           AMLDRL L+   L G+   +RQ+  L DP+G+V D   L SG+ + + RVPLGV+G+IYE
Sbjct: 70  AMLDRLTLSDKALNGMVTGLRQIAALKDPIGEVSDLSPLPSGIEVGKMRVPLGVVGIIYE 129

Query: 121 ARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQDALKSCGLPAGAVQAIDNPD 180
           +RPNVT+D A+LCLK+GNA ILRGG E   +N A    IQ  LK+  LP  AVQ +   D
Sbjct: 130 SRPNVTIDAAALCLKSGNAAILRGGSEARHSNQALAQCIQAGLKAADLPEAAVQVVPTTD 189

Query: 181 RALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVITGGIGVCHIYVDESVEIAEALK 240
           RA V E++ M +++D++IPRGG GL +     + +PVI    G+CH+Y+D++ +  +A +
Sbjct: 190 RAAVGELIAMPQFVDVIIPRGGKGLIERISADARVPVIKHLDGICHVYIDDAADAEKAHR 249

Query: 241 VIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQMAESGVTLHADAAALAQLQAGPAK 300
           V  NAKT R   CN +ETLLV ++ A   LP L++   + GV L       A+ +   A+
Sbjct: 250 VAFNAKTHRYGVCNAMETLLVAESRAAEILPRLAQAFEQKGVELR----GCARTREIIAE 305

Query: 301 VVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIREHGTQHSDAILTRDMRNAQRFVNEVD 360
           + A   E++  E+L   L++K+V+D   A+AHI ++ + H+++I+T ++  A+RF+ +VD
Sbjct: 306 MKAATDEDWATEYLDAILSIKVVADASAAMAHIAQYSSGHTESIITENLTTARRFLAQVD 365

Query: 361 SSAVYVNASTRFTDGGQFGLGAEVAVSTQKLHARGPMGLEALTTYKWIGIGDYTIR 416
           SS+V VNASTRF DG ++GLGAE+ +ST K HARGP+GLE LT+ K+I  GD TIR
Sbjct: 366 SSSVMVNASTRFADGFEYGLGAEIGISTDKFHARGPVGLEGLTSQKYIVFGDGTIR 421


Lambda     K      H
   0.317    0.133    0.369 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 423
Number of extensions: 14
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 417
Length of database: 422
Length adjustment: 32
Effective length of query: 385
Effective length of database: 390
Effective search space:   150150
Effective search space used:   150150
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 50 (23.9 bits)

Align candidate WP_019895556.1 A377_RS0107085 (glutamate-5-semialdehyde dehydrogenase)
to HMM TIGR00407 (proA: glutamate-5-semialdehyde dehydrogenase (EC 1.2.1.41))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00407.hmm
# target sequence database:        /tmp/gapView.19725.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00407  [M=398]
Accession:   TIGR00407
Description: proA: glutamate-5-semialdehyde dehydrogenase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
   3.5e-152  492.7   2.8   4.1e-152  492.5   2.8    1.0  1  lcl|NCBI__GCF_000384235.1:WP_019895556.1  A377_RS0107085 glutamate-5-semia


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000384235.1:WP_019895556.1  A377_RS0107085 glutamate-5-semialdehyde dehydrogenase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  492.5   2.8  4.1e-152  4.1e-152       1     398 []      18     411 ..      18     411 .. 0.99

  Alignments for each domain:
  == domain 1  score: 492.5 bits;  conditional E-value: 4.1e-152
                                 TIGR00407   1 akeaalklaqlstaeknealskiadeLkaeaelilaanakdiaaakenGladalldrLlLteeklksia 69 
                                               a+ aa++l    ta+kn al++ia e++++ae++  +na+d+ a+k+nGl  a+ldrL+L+++ l++++
  lcl|NCBI__GCF_000384235.1:WP_019895556.1  18 ARTAATQLSVADTAQKNAALEAIAVEIERRAEFLQSENARDLDAGKANGLDAAMLDRLTLSDKALNGMV 86 
                                               899****************************************************************** PP

                                 TIGR00407  70 ddvkdvieLadPvGkvieareldeGLklervrvPlGvlgviyearPevivdvasLclktGnaviLkGgk 138
                                                ++++++ L+dP+G+v +   l +G+ + ++rvPlGv+g+iye+rP+v++d+a+Lclk+Gna+iL+Gg+
  lcl|NCBI__GCF_000384235.1:WP_019895556.1  87 TGLRQIAALKDPIGEVSDLSPLPSGIEVGKMRVPLGVVGIIYESRPNVTIDAAALCLKSGNAAILRGGS 155
                                               ********************************************************************* PP

                                 TIGR00407 139 eavrsnkalveviqdaleqtglpveavqliedpdreevkellkldeyvdlliPrGgnelvklikeesti 207
                                               ea +sn+al++ iq  l+ + lp  avq++ ++dr+ v el+ + ++vd++iPrGg++l++ i  ++++
  lcl|NCBI__GCF_000384235.1:WP_019895556.1 156 EARHSNQALAQCIQAGLKAADLPEAAVQVVPTTDRAAVGELIAMPQFVDVIIPRGGKGLIERISADARV 224
                                               ********************************************************************* PP

                                 TIGR00407 208 PvlehadGvChiyldesadlakakkvivdaktqrPstCnaietLLvnkaiaeefleeLekqleekgvel 276
                                               Pv++h dG+Ch+y+d+ ad +ka++v  +akt+r  +Cna+etLLv ++ a+e l++L+++ e+kgvel
  lcl|NCBI__GCF_000384235.1:WP_019895556.1 225 PVIKHLDGICHVYIDDAADAEKAHRVAFNAKTHRYGVCNAMETLLVAESRAAEILPRLAQAFEQKGVEL 293
                                               ********************************************************************* PP

                                 TIGR00407 277 radalvlkllelekateaevskedfdkeflsldLsvkivedleeaiehirqygtkhsdailtedkknae 345
                                               r+ a + +++   ka    +++ed+ +e+l+ +Ls+k+v d  +a++hi qy++ h+++i+te+ ++a+
  lcl|NCBI__GCF_000384235.1:WP_019895556.1 294 RGCARTREIIAEMKA----ATDEDWATEYLDAILSIKVVADASAAMAHIAQYSSGHTESIITENLTTAR 358
                                               ********9987744....4589********************************************** PP

                                 TIGR00407 346 kfvkevdsaavyvnastrfadGfrfGfGaevgistqklharGPvGLeaLvsyk 398
                                               +f+ +vds++v vnastrfadGf++G+Gae+gist+k+harGPvGLe+L+s k
  lcl|NCBI__GCF_000384235.1:WP_019895556.1 359 RFLAQVDSSSVMVNASTRFADGFEYGLGAEIGISTDKFHARGPVGLEGLTSQK 411
                                               **************************************************986 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (398 nodes)
Target sequences:                          1  (422 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.02u 0.01s 00:00:00.03 Elapsed: 00:00:00.01
# Mc/sec: 9.05
//
[ok]

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis