Alignments for a candidate for cysE in Methyloferula stellata AR4T

GapMind for Amino acid biosynthesis

Alignments for a candidate for cysE in Methyloferula stellata AR4T

Align serine O-acetyltransferase (EC 2.3.1.30) (characterized)
to candidate WP_020176769.1 A3OQ_RS0117705 serine O-acetyltransferase

Query= BRENDA::C4IRW0
         (281 letters)



>NCBI__GCF_000385335.1:WP_020176769.1
          Length = 274

 Score =  336 bits (862), Expect = 3e-97
 Identities = 162/258 (62%), Positives = 200/258 (77%)

Query: 20  LDQVDPIWHSIRAEAEEATRNDPVLGAFLYATILNQPSLEEAVMHRIAERLGHPDVSADI 79
           ++ VDPI+  +R EAEE    +P +   + +TILN  +LE A++HR+A RLGHP+V  D+
Sbjct: 10  IESVDPIFARMRTEAEETIHREPEISGIMMSTILNHDTLESAIIHRLAARLGHPEVPGDL 69

Query: 80  LRQTFDTMLEANPEWSHVLRVDIQAVYDRDPAYSRFMDPVLYLKGFHAIQTHRLAHWLYK 139
           +R+T+  +L  +P  +   R D+ AV DRDPA  R ++PVLY KGFHAIQTHRLAH L+ 
Sbjct: 70  IRKTYLDVLAHDPSIAEAFRADLVAVVDRDPACVRVIEPVLYFKGFHAIQTHRLAHALWN 129

Query: 140 QGRKDFAYYLQSRSSSIFQTDIHPAARLGSGLFLDHATGLVVGETAVVEDNVSILHGVTL 199
            GR+DFA YLQSRSS +FQTDIHPAA+ G  +FLDHATGLVVG TAV+ED VS+L  VTL
Sbjct: 130 AGRRDFALYLQSRSSEVFQTDIHPAAQFGKSIFLDHATGLVVGSTAVIEDGVSMLQDVTL 189

Query: 200 GGTGKSSGDRHPKIRQGVLIGAGAKILGNIQVGQCSKIAAGSVVLKSVPHNVTVAGVPAR 259
           GGTGK  GDRHPKIR GVLIGAGAKILGNI+VG C++IAAGSVVL  VP+N TVAGVPA+
Sbjct: 190 GGTGKEKGDRHPKIRHGVLIGAGAKILGNIEVGHCARIAAGSVVLHPVPNNKTVAGVPAK 249

Query: 260 IIGETGCTEPSRVMDQML 277
           I+GE GC EP+  MDQ+L
Sbjct: 250 IVGEAGCAEPALSMDQIL 267


Lambda     K      H
   0.320    0.135    0.403 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 332
Number of extensions: 9
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 281
Length of database: 274
Length adjustment: 25
Effective length of query: 256
Effective length of database: 249
Effective search space:    63744
Effective search space used:    63744
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 47 (22.7 bits)

Align candidate WP_020176769.1 A3OQ_RS0117705 (serine O-acetyltransferase)
to HMM TIGR01172 (cysE: serine O-acetyltransferase (EC 2.3.1.30))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR01172.hmm
# target sequence database:        /tmp/gapView.16594.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01172  [M=162]
Accession:   TIGR01172
Description: cysE: serine O-acetyltransferase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
    4.5e-71  224.2   1.1    2.6e-70  221.7   1.0    1.9  2  lcl|NCBI__GCF_000385335.1:WP_020176769.1  A3OQ_RS0117705 serine O-acetyltr


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000385335.1:WP_020176769.1  A3OQ_RS0117705 serine O-acetyltransferase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 ?    0.4   0.0     0.032     0.032       4      40 ..      23      60 ..      20      66 .. 0.79
   2 !  221.7   1.0   2.6e-70   2.6e-70       2     162 .]      89     249 ..      88     249 .. 0.99

  Alignments for each domain:
  == domain 1  score: 0.4 bits;  conditional E-value: 0.032
                                 TIGR01172  4 dlkavlerDPaaes.alevlllykglhallayrlahal 40
                                              + +++++r+P ++  +++ +l++ +l + +++rla++l
  lcl|NCBI__GCF_000385335.1:WP_020176769.1 23 EAEETIHREPEISGiMMSTILNHDTLESAIIHRLAARL 60
                                              56778899998876156789999**********99876 PP

  == domain 2  score: 221.7 bits;  conditional E-value: 2.6e-70
                                 TIGR01172   2 kedlkavlerDPaaesalevlllykglhallayrlahalykrklkllarllselvrvltgvdihPaaki 70 
                                               ++dl av++rDPa+ +++e++l++kg+ha++++rlahal++ +++ +a +l+++++ +++ dihPaa++
  lcl|NCBI__GCF_000385335.1:WP_020176769.1  89 RADLVAVVDRDPACVRVIEPVLYFKGFHAIQTHRLAHALWNAGRRDFALYLQSRSSEVFQTDIHPAAQF 157
                                               6799***************************************************************** PP

                                 TIGR01172  71 grgvliDhatGvviGetavigddvsiyqgvtLGgtgkekgkRhPtvkegvvigagakvLGnievgenak 139
                                               g+ +++DhatG+v+G+tavi+d vs++q+vtLGgtgkekg+RhP++++gv+igagak+LGnievg+ a+
  lcl|NCBI__GCF_000385335.1:WP_020176769.1 158 GKSIFLDHATGLVVGSTAVIEDGVSMLQDVTLGGTGKEKGDRHPKIRHGVLIGAGAKILGNIEVGHCAR 226
                                               ********************************************************************* PP

                                 TIGR01172 140 iGansvvlkdvpaeatvvGvpar 162
                                               i a+svvl++vp++ tv+Gvpa+
  lcl|NCBI__GCF_000385335.1:WP_020176769.1 227 IAAGSVVLHPVPNNKTVAGVPAK 249
                                               *********************97 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (162 nodes)
Target sequences:                          1  (274 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.00u 0.00s 00:00:00.00 Elapsed: 00:00:00.00
# Mc/sec: 8.32
//
[ok]

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis