Align Dihydroxy-acid dehydratase, mitochondrial; DAD; EC 4.2.1.9 (characterized)
to candidate WP_020562313.1 A3OW_RS0104905 dihydroxy-acid dehydratase
Query= SwissProt::P39522 (585 letters) >NCBI__GCF_000372865.1:WP_020562313.1 Length = 565 Score = 613 bits (1580), Expect = e-180 Identities = 305/563 (54%), Positives = 404/563 (71%), Gaps = 8/563 (1%) Query: 22 KLNKYSYIITEPKGQGASQAMLYATGFKKEDFKKPQVGVGSCWWSGNPCNMHLLDLNNRC 81 K YS + + + S+AML+A GF EDFKKPQ+G+ S W PCNMH+ L + Sbjct: 8 KTRTYSSQVVDGMERAPSRAMLHAVGFTNEDFKKPQIGIASTWSMVTPCNMHINKLADNA 67 Query: 82 SQSIEKAGLKAMQFNTIGVSDGISMGTKGMRYSLQSREIIADSFETIMMAQHYDANIAIP 141 ++ +++ G KA+ FNTI +SDGISMGT+GM+YSL SRE+IADS ET++ Q +D +AI Sbjct: 68 ARGVDQTGGKAVIFNTITISDGISMGTEGMKYSLVSREVIADSIETVVGCQGFDGIVAIG 127 Query: 142 SCDKNMPGVMMAMGRHNRPSIMVYGGTILPGHPTCGSSKISKNIDIVSAFQSYGEYISKQ 201 CDKNMPG M+A+ R NRP+I VYGGTILPG C K +D+VS F++ G + + Sbjct: 128 GCDKNMPGCMIALSRLNRPAIFVYGGTILPG---CHKDK---KLDVVSVFEAVGARANNK 181 Query: 202 FTEEEREDVVEHACPGPGSCGGMYTANTMASAAEVLGLTIPNSSSFPAVSKEKLAECDNI 261 + E + A PG GSCGGMYTANTMASA E LG+++PNSS+ AVS++K +C+ Sbjct: 182 IDDAELAAIEAKAIPGAGSCGGMYTANTMASAIEALGMSLPNSSAQAAVSEDKRLDCERA 241 Query: 262 GEYIKKTMELGILPRDILTKEAFENAITYVVATGGSTNAVLHLVAVAHSAGVKLSPDDFQ 321 G + + ++ GI PRDI+TKEAFENAIT V+A GGSTNAVLHL+A+A++AGV L+ DDF Sbjct: 242 GAAVLELLKKGIKPRDIMTKEAFENAITVVIALGGSTNAVLHLLAMANAAGVDLTLDDFT 301 Query: 322 RISDTTPLIGDFKPSGKYVMADLINVGGTQSVIKYLYENNMLHGNTMTVTGDTLAERAKK 381 RI P++ D KPSG+Y MA+LI +GG Q ++K L + +LHG+ +TVTG TLAE Sbjct: 302 RIGKNVPMVADLKPSGRYQMAELIEIGGIQPLMKELLDRGLLHGHCLTVTGKTLAENLAD 361 Query: 382 APSLPEGQEIIKPLSHPIKANGHLQILYGSLAPGGAVGKITGKEGTYFKGRARVFEEEGA 441 PEGQ++I PL PIK + HL +LYG+LA GAV KITGKEG F G+A+VF+ E Sbjct: 362 VKPYPEGQDMIHPLDQPIKKDSHLVVLYGNLAAEGAVAKITGKEGLVFTGKAKVFDAEEQ 421 Query: 442 FIEALERGEIKKGEKTVVVIRYEGPRGAPGMPEMLKPSSALMGYGLGKDVALLTDGRFSG 501 ++A+ G+I KG+ V+VIRYEGP+G PGM EML P+SA+MG GLGK+VAL+TDGRFSG Sbjct: 422 ALQAILNGDIVKGD--VIVIRYEGPKGGPGMREMLSPTSAVMGKGLGKEVALITDGRFSG 479 Query: 502 GSHGFLIGHIVPEAAEGGPIGLVRDGDEIIIDADNNKIDLLVSDKEMAQRKQSWVAPPPR 561 G+HGF++GHI PEA GG + +V++GDEI IDA+ ++ L V++ E+A+R W P PR Sbjct: 480 GTHGFVVGHITPEAYTGGALAIVQNGDEITIDAETRQLTLHVNEHEIARRFDKWKQPAPR 539 Query: 562 YTRGTLSKYAKLVSNASNGCVLD 584 YTRG L+KYAKLVS+AS G V D Sbjct: 540 YTRGVLAKYAKLVSSASKGAVTD 562 Lambda K H 0.315 0.134 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 980 Number of extensions: 52 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 585 Length of database: 565 Length adjustment: 36 Effective length of query: 549 Effective length of database: 529 Effective search space: 290421 Effective search space used: 290421 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 53 (25.0 bits)
Align candidate WP_020562313.1 A3OW_RS0104905 (dihydroxy-acid dehydratase)
to HMM TIGR00110 (ilvD: dihydroxy-acid dehydratase (EC 4.2.1.9))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00110.hmm # target sequence database: /tmp/gapView.14982.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00110 [M=543] Accession: TIGR00110 Description: ilvD: dihydroxy-acid dehydratase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 1e-239 782.5 9.3 1.2e-239 782.3 9.3 1.0 1 lcl|NCBI__GCF_000372865.1:WP_020562313.1 A3OW_RS0104905 dihydroxy-acid de Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_000372865.1:WP_020562313.1 A3OW_RS0104905 dihydroxy-acid dehydratase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 782.3 9.3 1.2e-239 1.2e-239 1 542 [. 24 562 .. 24 563 .. 1.00 Alignments for each domain: == domain 1 score: 782.3 bits; conditional E-value: 1.2e-239 TIGR00110 1 aarallkatGlkdedlekPiiavvnsyteivPghvhlkdlaklvkeeieaaGgvakefntiavsDGiam 69 ++ra+l+a+G+++ed++kP+i+++++++ ++P+++h+++la+ ++++++++Gg+a+ fnti++sDGi+m lcl|NCBI__GCF_000372865.1:WP_020562313.1 24 PSRAMLHAVGFTNEDFKKPQIGIASTWSMVTPCNMHINKLADNAARGVDQTGGKAVIFNTITISDGISM 92 589****************************************************************** PP TIGR00110 70 gheGmkysLpsreiiaDsvetvvkahalDalvvissCDkivPGmlmaalrlniPaivvsGGpmeagktk 138 g+eGmkysL+sre+iaDs+etvv + +D++v+i+ CDk++PG ++a rln+Pai+v+GG++ +g k lcl|NCBI__GCF_000372865.1:WP_020562313.1 93 GTEGMKYSLVSREVIADSIETVVGCQGFDGIVAIGGCDKNMPGCMIALSRLNRPAIFVYGGTILPGCHK 161 *******************************************************************99 PP TIGR00110 139 lsekidlvdvfeavgeyaagklseeeleeiersacPtagsCsGlftansmacltealGlslPgsstlla 207 +k+d+v+vfeavg+ a++k++++el +ie a P+agsC+G++tan+ma++ ealG+slP+ss+ +a lcl|NCBI__GCF_000372865.1:WP_020562313.1 162 -DKKLDVVSVFEAVGARANNKIDDAELAAIEAKAIPGAGSCGGMYTANTMASAIEALGMSLPNSSAQAA 229 .8******************************************************************* PP TIGR00110 208 tsaekkelakksgkrivelvkknikPrdiltkeafenaitldlalGGstntvLhllaiakeagvklsld 276 +s++k+ ++++g+ + el+kk ikPrdi+tkeafenait+++alGGstn+vLhlla+a+ agv+l+ld lcl|NCBI__GCF_000372865.1:WP_020562313.1 230 VSEDKRLDCERAGAAVLELLKKGIKPRDIMTKEAFENAITVVIALGGSTNAVLHLLAMANAAGVDLTLD 298 ********************************************************************* PP TIGR00110 277 dfdrlsrkvPllaklkPsgkkviedlhraGGvsavlkeldkegllhkdaltvtGktlaetlekvkvlrv 345 df r+ ++vP++a+lkPsg++ +++l + GG++ ++kel +gllh ++ltvtGktlae+l++vk ++ lcl|NCBI__GCF_000372865.1:WP_020562313.1 299 DFTRIGKNVPMVADLKPSGRYQMAELIEIGGIQPLMKELLDRGLLHGHCLTVTGKTLAENLADVKPYPE 367 ********************************************************************* PP TIGR00110 346 dqdvirsldnpvkkegglavLkGnlaeeGavvkiagveedilkfeGpakvfeseeealeailggkvkeG 414 +qd+i++ld+p+kk+++l vL+Gnla eGav+ki+g+e l+f+G+akvf+ ee+al+ail+g++ +G lcl|NCBI__GCF_000372865.1:WP_020562313.1 368 GQDMIHPLDQPIKKDSHLVVLYGNLAAEGAVAKITGKEG--LVFTGKAKVFDAEEQALQAILNGDIVKG 434 ***************************************..9*************************** PP TIGR00110 415 dvvviryeGPkGgPGmremLaPtsalvglGLgkkvaLitDGrfsGgtrGlsiGhvsPeaaegGaialve 483 dv+viryeGPkGgPGmremL Ptsa++g GLgk+vaLitDGrfsGgt+G+++Gh++Pea++gGa+a+v+ lcl|NCBI__GCF_000372865.1:WP_020562313.1 435 DVIVIRYEGPKGGPGMREMLSPTSAVMGKGLGKEVALITDGRFSGGTHGFVVGHITPEAYTGGALAIVQ 503 ********************************************************************* PP TIGR00110 484 dGDkikiDienrkldlevseeelaerrakakkkearevkgaLakyaklvssadkGavld 542 +GD+i+iD+e r+l l+v+e+e+a+r +k+k++ +r+++g+Lakyaklvssa+kGav+d lcl|NCBI__GCF_000372865.1:WP_020562313.1 504 NGDEITIDAETRQLTLHVNEHEIARRFDKWKQPAPRYTRGVLAKYAKLVSSASKGAVTD 562 *********************************************************97 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (543 nodes) Target sequences: 1 (565 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.03u 0.01s 00:00:00.04 Elapsed: 00:00:00.03 # Mc/sec: 9.39 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory