Alignments for a candidate for thrC in Methylosarcina fibrata AML-C10

GapMind for Amino acid biosynthesis

Alignments for a candidate for thrC in Methylosarcina fibrata AML-C10

Align threonine synthase (EC 4.2.3.1) (characterized)
to candidate WP_020562611.1 A3OW_RS0106470 threonine synthase

Query= BRENDA::O66740
         (352 letters)



>NCBI__GCF_000372865.1:WP_020562611.1
          Length = 364

 Score =  407 bits (1045), Expect = e-118
 Identities = 195/354 (55%), Positives = 267/354 (75%), Gaps = 7/354 (1%)

Query: 4   WQGIIKQYKKYLPVDENTPIVTLYEGNTPLIEADNLARAIGFKGKIYLKYEGLNPTGSFK 63
           + G+I++Y+  LPV  +T I++L EGNTPLI   N+ R  G +  I++K+EGLNPTGSFK
Sbjct: 7   YTGLIERYRDRLPVSASTRIISLNEGNTPLIRLQNIPRLTGKEVDIHVKFEGLNPTGSFK 66

Query: 64  DRGMTLAISKAVEAGKRAVICASTGNTSASAAAYAARAGLRAYVLLPKGAVAIGKLSQAM 123
           DRGMT+A++KAVE G RA+ICASTGNTSASAAAYAARAG++A+V++P+G +A+GKL+Q +
Sbjct: 67  DRGMTMAVTKAVEEGSRAIICASTGNTSASAAAYAARAGIKAFVIIPEGKIALGKLAQTL 126

Query: 124 IYGAKVLAIQGTFDDALNIVRKIGENFPVEIVNSVNPYRIEGQKTAAFEICDTLGEAPDY 183
           +Y A ++ I+G FD  + +V++I ++ PV IVNS+NP+RI+GQKTA+FEI D LG+APDY
Sbjct: 127 MYDATIIQIRGNFDQGMTLVKEIADHAPVTIVNSINPFRIDGQKTASFEIIDALGDAPDY 186

Query: 184 HFIPVGNAGNITAYWKGFKIYYEEGK-----ITKLPRMMGWQAEGAAPIVKGYPIKNPQT 238
           H +PVGNAGNITAYWKG+K Y  + +       K P M G+QA GAAP + G+PI++P+T
Sbjct: 187 HCLPVGNAGNITAYWKGYKEYSTDSETHQAVTRKRPVMCGYQAAGAAPFLAGHPIEHPET 246

Query: 239 IATAIKIGNPYSWKSALKAAQESGGKIDAVSDSEILYAYKLIASTEGVFCEPASAASVAG 298
           +ATAI+IGNP SW  A  A +ESGG  D  +D EIL A K+++  EG+FCEPASA S+AG
Sbjct: 247 VATAIRIGNPQSWDYAWAAQKESGGWFDKFTDEEILAAQKMLSQYEGIFCEPASATSLAG 306

Query: 299 LIKLVREGFFKGGEVVTCTLTGNGLKDPDTAIKVC--EEPITVPPDFDEVVKVL 350
            +K +R G    G  + CTLTG GLKDPDTAI+ C   +P+T+  + + V + +
Sbjct: 307 ALKDIRSGKIPEGSRIVCTLTGVGLKDPDTAIRQCAATQPLTIEAELNAVKRAI 360


Lambda     K      H
   0.317    0.136    0.400 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 454
Number of extensions: 21
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 352
Length of database: 364
Length adjustment: 29
Effective length of query: 323
Effective length of database: 335
Effective search space:   108205
Effective search space used:   108205
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 49 (23.5 bits)

Align candidate WP_020562611.1 A3OW_RS0106470 (threonine synthase)
to HMM TIGR00260 (thrC: threonine synthase (EC 4.2.3.1))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00260.hmm
# target sequence database:        /tmp/gapView.25696.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00260  [M=340]
Accession:   TIGR00260
Description: thrC: threonine synthase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
    1.9e-96  309.1   0.0    2.2e-96  308.9   0.0    1.0  1  lcl|NCBI__GCF_000372865.1:WP_020562611.1  A3OW_RS0106470 threonine synthas


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000372865.1:WP_020562611.1  A3OW_RS0106470 threonine synthase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  308.9   0.0   2.2e-96   2.2e-96       1     340 []      11     335 ..      11     335 .. 0.97

  Alignments for each domain:
  == domain 1  score: 308.9 bits;  conditional E-value: 2.2e-96
                                 TIGR00260   1 vvryreilevt.ekdlvdlaegstelfrspklaeevga.enlyvkelfhgPtlaFKDrglqfvavlltk 67 
                                               ++ryr  l+v+  +++++l+eg+t+l+r +++++  g+  +++vk ++++Pt++FKDrg   +++++tk
  lcl|NCBI__GCF_000372865.1:WP_020562611.1  11 IERYRDRLPVSaSTRIISLNEGNTPLIRLQNIPRLTGKeVDIHVKFEGLNPTGSFKDRG---MTMAVTK 76 
                                               78*******************************99999899******************...******* PP

                                 TIGR00260  68 alelgnetvlcAtsGdtgaaaaealagkanvkvvvLyPkgkispvkeklvtalaenakvlaikGdFDda 136
                                               a e+g  +++cA++G+t+a+aa a+a++a++k++v++P+gki+   +kl+++l++ a++++i+G+FD+ 
  lcl|NCBI__GCF_000372865.1:WP_020562611.1  77 AVEEGSRAIICASTGNTSASAA-AYAARAGIKAFVIIPEGKIA--LGKLAQTLMYDATIIQIRGNFDQG 142
                                               **********************.********************..************************ PP

                                 TIGR00260 137 qdlvkeifedkeklklnsvNsinparieaqktyafeiveqlgkespdkvvvpvp.sgnfgailkGflek 204
                                                +lvkei+++ + + +ns+N   p+ri +qkt+ fei+++lg ++pd  ++pv+ +gn++a++kG++e+
  lcl|NCBI__GCF_000372865.1:WP_020562611.1 143 MTLVKEIADHAPVTIVNSIN---PFRIDGQKTASFEIIDALG-DAPDYHCLPVGnAGNITAYWKGYKEY 207
                                               ********998877777777...*******************.************************** PP

                                 TIGR00260 205 kelg.........lpieklaiaaegaadivrrflksgdlepkedkeTlstAmdignpsnveralelarr 264
                                                +           +p  + + +a gaa+++          p+e++eT++tA++ignp ++++a+++ ++
  lcl|NCBI__GCF_000372865.1:WP_020562611.1 208 STDSethqavtrkRP-VMCGYQAAGAAPFLAGH-------PIEHPETVATAIRIGNPQSWDYAWAAQKE 268
                                               999899999999999.99999****98888777.......***************************** PP

                                 TIGR00260 265 slgnledlkesvsdeeileaikklaeeegyllephtavavaalkklvekgvsatadpaKFeevvealtg 333
                                               s+g +++     +deeil a+k l + eg+++ep++a+++a+ +k + +g   +++++  +++v+ ltg
  lcl|NCBI__GCF_000372865.1:WP_020562611.1 269 SGGWFDKF----TDEEILAAQKMLSQYEGIFCEPASATSLAGALKDIRSG---KIPEG--SRIVCTLTG 328
                                               ********....**************************************...*****..********* PP

                                 TIGR00260 334 nklkdpe 340
                                                +lkdp+
  lcl|NCBI__GCF_000372865.1:WP_020562611.1 329 VGLKDPD 335
                                               *****97 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (340 nodes)
Target sequences:                          1  (364 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.00u 0.01s 00:00:00.01 Elapsed: 00:00:00.01
# Mc/sec: 9.84
//
[ok]

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis