Alignments for a candidate for ilvI in Methylohalobius crimeensis 10Ki

GapMind for Amino acid biosynthesis

Alignments for a candidate for ilvI in Methylohalobius crimeensis 10Ki

Align acetohydroxyacid synthase subunit B (EC 2.2.1.6) (characterized)
to candidate WP_022947184.1 H035_RS0101175 acetolactate synthase 3 large subunit

Query= metacyc::MONOMER-18810
         (585 letters)



>NCBI__GCF_000421465.1:WP_022947184.1
          Length = 576

 Score =  780 bits (2014), Expect = 0.0
 Identities = 376/565 (66%), Positives = 448/565 (79%), Gaps = 3/565 (0%)

Query: 18  EMIGAEILVHALAEEGVEYVWGYPGGAVLYIYDELHKQTKFEHILVRHEQAAVHAADGYA 77
           E+ GAEI++ AL +EGVE+++GYPGGAVL+IYD L  Q   +H+LVRHEQ A H ADGYA
Sbjct: 2   ELSGAEIVIQALKDEGVEFIFGYPGGAVLHIYDALFGQEDVKHVLVRHEQGAAHMADGYA 61

Query: 78  RATGKVGVALVTSGPGVTNAVTGIATAYLDSIPMVVITGNVPTHAIGQDAFQECDTVGIT 137
           RATGK GV L TSGPG TN +TGIATA++DSIP+VV+TG V T  IG DAFQECDTVGIT
Sbjct: 62  RATGKPGVVLATSGPGATNLITGIATAHMDSIPLVVLTGQVATPVIGSDAFQECDTVGIT 121

Query: 138 RPIVKHNFLVKDVRDLAATIKKAFFIAATGRPGPVVVDIPKDVSRNACK--YEYPKSIDM 195
            P VKHNFLVKD R LA TIKKAF+IA TGRPGPVVVDIPKDV+    K  Y YP ++ +
Sbjct: 122 LPCVKHNFLVKDRRQLAETIKKAFYIATTGRPGPVVVDIPKDVTDPNVKIPYHYPDNVSL 181

Query: 196 RSYNPVNKGHSGQIRKAVALLQGAERPYIYTGGGVVLANASDELRQLAALTGHPVTNTLM 255
           RSY P  +G+  QIRKA  L+  A RP +YTGGGV+L  A+ EL +L    G P+TNTLM
Sbjct: 182 RSYRPKIQGNPRQIRKAAELILEARRPMLYTGGGVILGEAAAELTELTRWLGFPITNTLM 241

Query: 256 GLGAFPGTSKQFVGMLGMHGTYEANMAMQNCDVLIAIGARFDDRVIGNPAHFTSQARKII 315
           GLGA+PG  +QF+GMLGMHGT+EANMAM  CDVLIA+GARFDDRV G    F   A KII
Sbjct: 242 GLGAYPGADRQFLGMLGMHGTFEANMAMHECDVLIAVGARFDDRVTGKLDEFCPYA-KII 300

Query: 316 HIDIDPSSISKRVKVDIPIVGNVKDVLQELIAQIKASDIKPKREALAKWWEQIEQWRSVD 375
           HID+DP+SI+K V VDIPIVG VK VL  LI ++K++  +P  +ALA WWEQIE WR++D
Sbjct: 301 HIDVDPASIAKTVPVDIPIVGQVKPVLAALIERLKSTGKRPNPDALAAWWEQIENWRAID 360

Query: 376 CLKYDRSSEIIKPQYVVEKIWELTKGDAFICSDVGQHQMWAAQFYKFDEPRRWINSGGLG 435
           CL+Y++ S +IKPQYV+E++WE T+G A++ SDVGQHQMW AQ+YKFD PR WINSGGLG
Sbjct: 361 CLRYEQDSPLIKPQYVIEQLWEATRGKAYVTSDVGQHQMWVAQYYKFDRPRHWINSGGLG 420

Query: 436 TMGVGLPYAMGIKKAFPEKEVVTITGEGSIQMCIQELSTCLQYDTPVKICSLNNGYLGMV 495
           TMG G+P A+G+K  FPE++VV +TGE SIQMCIQELST LQY  P+KI +LNN Y+GMV
Sbjct: 421 TMGFGMPAAIGVKLGFPEEDVVCVTGEASIQMCIQELSTALQYKAPIKIVNLNNRYMGMV 480

Query: 496 RQWQEIEYDNRYSHSYMDALPDFVKLAEAYGHVGMRVEKTSDVEPALREAFRLKDRTVFL 555
           RQWQE  Y++RYSHSYMD LPDFVKLAEAYGHVGMR+EK  DV PAL EAF++KDR VFL
Sbjct: 481 RQWQEFSYESRYSHSYMDTLPDFVKLAEAYGHVGMRIEKPEDVRPALEEAFQMKDRLVFL 540

Query: 556 DFQTDPTENVWPMVQAGKGISEMLL 580
           D  TD TENV+PMV+AGK   +M L
Sbjct: 541 DCITDRTENVYPMVEAGKAQHQMRL 565


Lambda     K      H
   0.319    0.135    0.407 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1063
Number of extensions: 40
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 585
Length of database: 576
Length adjustment: 36
Effective length of query: 549
Effective length of database: 540
Effective search space:   296460
Effective search space used:   296460
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 53 (25.0 bits)

Align candidate WP_022947184.1 H035_RS0101175 (acetolactate synthase 3 large subunit)
to HMM TIGR00118 (ilvB: acetolactate synthase, large subunit, biosynthetic type (EC 2.2.1.6))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00118.hmm
# target sequence database:        /tmp/gapView.27773.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00118  [M=557]
Accession:   TIGR00118
Description: acolac_lg: acetolactate synthase, large subunit, biosynthetic type
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
   2.9e-266  870.2   0.0   3.6e-266  869.9   0.0    1.0  1  lcl|NCBI__GCF_000421465.1:WP_022947184.1  H035_RS0101175 acetolactate synt


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000421465.1:WP_022947184.1  H035_RS0101175 acetolactate synthase 3 large subunit
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  869.9   0.0  3.6e-266  3.6e-266       1     553 [.       3     563 ..       3     566 .. 0.98

  Alignments for each domain:
  == domain 1  score: 869.9 bits;  conditional E-value: 3.6e-266
                                 TIGR00118   1 lkgaeilveslkkegvetvfGyPGGavlpiydaly.dselehilvrheqaaahaadGyarasGkvGvvl 68 
                                               l+gaei++++lk+egve +fGyPGGavl+iydal+ +++++h+lvrheq+aah+adGyara+Gk+Gvvl
  lcl|NCBI__GCF_000421465.1:WP_022947184.1   3 LSGAEIVIQALKDEGVEFIFGYPGGAVLHIYDALFgQEDVKHVLVRHEQGAAHMADGYARATGKPGVVL 71 
                                               68*********************************9********************************* PP

                                 TIGR00118  69 atsGPGatnlvtgiatayldsvPlvvltGqvatsliGsdafqeidilGitlpvtkhsflvkkaedlpei 137
                                               atsGPGatnl+tgiata++ds+PlvvltGqvat +iGsdafqe+d +Gitlp++kh+flvk+ ++l+e+
  lcl|NCBI__GCF_000421465.1:WP_022947184.1  72 ATSGPGATNLITGIATAHMDSIPLVVLTGQVATPVIGSDAFQECDTVGITLPCVKHNFLVKDRRQLAET 140
                                               ********************************************************************* PP

                                 TIGR00118 138 lkeafeiastGrPGPvlvdlPkdvteaeieleve..ekvelpgykptvkghklqikkaleliekakkPv 204
                                               +k+af+ia+tGrPGPv+vd+Pkdvt+++++++++  ++v+l++y+p+++g+++qi+ka+eli +a++P+
  lcl|NCBI__GCF_000421465.1:WP_022947184.1 141 IKKAFYIATTGRPGPVVVDIPKDVTDPNVKIPYHypDNVSLRSYRPKIQGNPRQIRKAAELILEARRPM 209
                                               *****************************99988789******************************** PP

                                 TIGR00118 205 llvGgGviiaeaseelkelaerlkipvtttllGlGafpedhplalgmlGmhGtkeanlavseadlliav 273
                                               l+ GgGvi +ea +el+el+  l  p+t tl+GlGa+p  ++++lgmlGmhGt ean+a++e+d+liav
  lcl|NCBI__GCF_000421465.1:WP_022947184.1 210 LYTGGGVILGEAAAELTELTRWLGFPITNTLMGLGAYPGADRQFLGMLGMHGTFEANMAMHECDVLIAV 278
                                               ********************************************************************* PP

                                 TIGR00118 274 GarfddrvtgnlakfapeakiihididPaeigknvkvdipivGdakkvleellkklkee....ekkeke 338
                                               Garfddrvtg+l++f+p akiihid+dPa+i+k+v vdipivG++k vl+ l+++lk++    + +   
  lcl|NCBI__GCF_000421465.1:WP_022947184.1 279 GARFDDRVTGKLDEFCPYAKIIHIDVDPASIAKTVPVDIPIVGQVKPVLAALIERLKSTgkrpNPDALA 347
                                               ********************************************************9998776444444 PP

                                 TIGR00118 339 .WlekieewkkeyilkldeeeesikPqkvikelskllkdeaivttdvGqhqmwaaqfyktkkprkfits 406
                                                W+e+ie+w++  +l++++++  ikPq+vi++l++ ++++a+vt+dvGqhqmw+aq+yk+++pr++i+s
  lcl|NCBI__GCF_000421465.1:WP_022947184.1 348 aWWEQIENWRAIDCLRYEQDSPLIKPQYVIEQLWEATRGKAYVTSDVGQHQMWVAQYYKFDRPRHWINS 416
                                               6******************************************************************** PP

                                 TIGR00118 407 gGlGtmGfGlPaalGakvakpeetvvavtGdgsfqmnlqelstiveydipvkivilnnellGmvkqWqe 475
                                               gGlGtmGfG+Paa+G+k++ pee+vv+vtG++s+qm +qelst+ +y+ p+kiv+lnn+++Gmv+qWqe
  lcl|NCBI__GCF_000421465.1:WP_022947184.1 417 GGLGTMGFGMPAAIGVKLGFPEEDVVCVTGEASIQMCIQELSTALQYKAPIKIVNLNNRYMGMVRQWQE 485
                                               ********************************************************************* PP

                                 TIGR00118 476 lfyeerysetklaselpdfvklaeayGvkgiriekpeeleeklkealesk.epvlldvevdkeeevlPm 543
                                               + ye rys++++ + lpdfvklaeayG++g+riekpe++  +l+ea+++k ++v+ld   d++e+v+Pm
  lcl|NCBI__GCF_000421465.1:WP_022947184.1 486 FSYESRYSHSYMDT-LPDFVKLAEAYGHVGMRIEKPEDVRPALEEAFQMKdRLVFLDCITDRTENVYPM 553
                                               *************5.********************************998799**************** PP

                                 TIGR00118 544 vapGagldel 553
                                               v  G++ +++
  lcl|NCBI__GCF_000421465.1:WP_022947184.1 554 VEAGKAQHQM 563
                                               ******9998 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (557 nodes)
Target sequences:                          1  (576 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.03u 0.01s 00:00:00.04 Elapsed: 00:00:00.03
# Mc/sec: 9.05
//
[ok]

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis