Alignments for a candidate for trpED in Haloglycomyces albus DSM 45210

GapMind for Amino acid biosynthesis

Alignments for a candidate for trpED in Haloglycomyces albus DSM 45210

Align candidate WP_025273780.1 HALAL_RS0109490 (anthranilate synthase component I)
to HMM TIGR01815 (anthranilate synthase (EC 4.1.3.27))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR01815.hmm
# target sequence database:        /tmp/gapView.22700.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01815  [M=717]
Accession:   TIGR01815
Description: TrpE-clade3: anthranilate synthase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
   7.3e-287  939.0   0.0   8.2e-287  938.8   0.0    1.0  1  lcl|NCBI__GCF_000527155.1:WP_025273780.1  HALAL_RS0109490 anthranilate syn


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000527155.1:WP_025273780.1  HALAL_RS0109490 anthranilate synthase component I
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  938.8   0.0  8.2e-287  8.2e-287       1     706 [.       4     703 ..       4     708 .] 0.95

  Alignments for each domain:
  == domain 1  score: 938.8 bits;  conditional E-value: 8.2e-287
                                 TIGR01815   1 yrtkGgvvvtrratevkletaleevvdalderrGallsssyeypGrykrwalgfvdppvalsasGrtlr 69 
                                               y+t Gg ++tr a  +++ + l+ + + +++rrG++lss++eypGry r+ l++v+p+ ++  sGrt++
  lcl|NCBI__GCF_000527155.1:WP_025273780.1   4 YTTAGGARITRTAATLNT-DDLDYISELVESRRGGVLSSGMEYPGRYSRFHLAYVNPAAEVVVSGRTVT 71 
                                               89**********999986.5699999999**************************************** PP

                                 TIGR01815  70 iealnerGevllpviaealkkvedlakleeaarrvtasvkspdevlaeeerskqpsvftvlravldlfa 138
                                                +alnerG+v+lpv   al++v+ ++        v+  v   + v++ee+rs+q +vft lra++  f 
  lcl|NCBI__GCF_000527155.1:WP_025273780.1  72 AQALNERGQVVLPVFEHALSQVAPVKVEG---DAVSIEVPTDPGVVTEEQRSRQLTVFTALRAIVSAFG 137
                                               ******************99998876544...446677777889************************8 PP

                                 TIGR01815 139 seedellGlyGafgydlafqfeaieqklerpddqrdlvlylpdelvvvdyyaakaflveydfitaagst 207
                                               + ed+ lGl+Gafgydla+qfe+ie   erp d rd vl+l de+ vvd   + a l +ydf  + +st
  lcl|NCBI__GCF_000527155.1:WP_025273780.1 138 C-EDPYLGLWGAFGYDLAYQFEPIEFAKERPSDVRDAVLHLADEVYVVDRKRETASLYRYDFEVDGAST 205
                                               7.79***************************************************************** PP

                                 TIGR01815 208 egleadgedepyrsdtsiepqadhkpGeyaklvekakeafrrGdlfevvpgqkffeaceaapsevferl 276
                                                gle++ge+ +yr+ ++++++    pG y+++v +ake fr G+lfevvpg +++++c++ p++ +erl
  lcl|NCBI__GCF_000527155.1:WP_025273780.1 206 VGLERTGEHFDYRPAEEVPAG--PTPGAYSEVVRQAKEEFREGNLFEVVPGHEMYTTCDS-PASFYERL 271
                                               *****************9987..57*********************************75.8899**** PP

                                 TIGR01815 277 kainpspyeffvnlGrgeylvgaspemfvrveGrrvetcpisGtikrGddaiddaaqilkllnskkdea 345
                                               k+ npspyeff+nlG++eylvg+spemfvrv+G+rvetcpisGti+rG+d+++da qil llns+k+ea
  lcl|NCBI__GCF_000527155.1:WP_025273780.1 272 KKSNPSPYEFFFNLGHDEYLVGTSPEMFVRVNGNRVETCPISGTIARGNDPLQDAGQILSLLNSEKEEA 340
                                               ********************************************************************* PP

                                 TIGR01815 346 eltmctdvdrndksrvcepGsvkviGrrqielysrlihtvdhveGrlrdeldaldaflshawavtvtGa 414
                                               eltmctdvdrndk+rvc+pGsv+viGrrqielys+lihtvdh+eGrlr+++da+dafl+h wavtvtGa
  lcl|NCBI__GCF_000527155.1:WP_025273780.1 341 ELTMCTDVDRNDKARVCVPGSVRVIGRRQIELYSHLIHTVDHIEGRLREDMDAFDAFLTHMWAVTVTGA 409
                                               ********************************************************************* PP

                                 TIGR01815 415 pkraalqfiekteksprrwyGGavGrlgfnGdlntGltlrtirlkdgiaevraGatllydsdpeaeeae 483
                                               pk +a++fie +e +prrwyGGavG++gf+G +ntGltlrt  ++dg++ vraGatlly sdp+ eeae
  lcl|NCBI__GCF_000527155.1:WP_025273780.1 410 PKTWAMRFIERNETTPRRWYGGAVGYVGFDGSMNTGLTLRTAHIRDGLTSVRAGATLLYYSDPDMEEAE 478
                                               ********************************************************************* PP

                                 TIGR01815 484 telkaaalieairdakaadaakees...avakvgeGkrillvdhedsfvhtlanylrqtGasvttlrhs 549
                                               t lka+al+ea    + + +++ ++   a  + g+G+r+ll+dhedsfv tla+y+rq  a vttlr +
  lcl|NCBI__GCF_000527155.1:WP_025273780.1 479 TFLKASALLEAAEGKHRMRPEPPAAidvAGGAAGTGRRVLLIDHEDSFVNTLADYFRQHDAEVTTLRYG 547
                                               ***********997777766554331003445669********************************** PP

                                 TIGR01815 550 va.eelldeerpdlvvlspGpGrpkdfdvketikaalardlpvfGvclGlqglveafGGeldvlaepvh 617
                                               +   ++ld+ +pdlvvlspGpGrp+df +++ +    +r+lpvfGvclGlq++ve  GG+l+ l+ep h
  lcl|NCBI__GCF_000527155.1:WP_025273780.1 548 FDlGQALDDINPDLVVLSPGPGRPTDFACQALLRDLERRELPVFGVCLGLQAMVEYTGGKLSLLDEPAH 616
                                               973689*************************************************************** PP

                                 TIGR01815 618 GkasrirvlepdvlfaglpeeltvGryhslfarsdrlpkelkvtaesedgvimaiehrklpvaavqfhp 686
                                               Gk+ +++v ++  lf++++++lt  ryhsl a+ d++   ++ t+ + d++ m+ie        vqfhp
  lcl|NCBI__GCF_000527155.1:WP_025273780.1 617 GKPGNVKVFGG-GLFSDVGDRLTTARYHSLHATIDQVQGGFV-TTSALDDIAMSIEDPMRGWYGVQFHP 683
                                               *********99.79***********************99986.55677889****************** PP

                                 TIGR01815 687 esiltlaGeaGlalienvvd 706
                                               esilt +   Gl+li+nv+ 
  lcl|NCBI__GCF_000527155.1:WP_025273780.1 684 ESILTSERASGLRLIGNVLR 703
                                               *****************985 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (717 nodes)
Target sequences:                          1  (708 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.04u 0.02s 00:00:00.06 Elapsed: 00:00:00.06
# Mc/sec: 7.82
//
[ok]

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis