Alignments for a candidate for hisN in Methylocapsa acidiphila B2

GapMind for Amino acid biosynthesis

Alignments for a candidate for hisN in Methylocapsa acidiphila B2

Align Histidinol-phosphatase [alternative form] (EC 3.1.3.15) (characterized)
to candidate WP_026607886.1 METAC_RS0116440 histidinol-phosphatase

Query= reanno::azobra:AZOBR_RS03845
         (260 letters)



>NCBI__GCF_000427445.1:WP_026607886.1
          Length = 265

 Score =  224 bits (572), Expect = 1e-63
 Identities = 120/259 (46%), Positives = 160/259 (61%), Gaps = 16/259 (6%)

Query: 14  ERLADASGPVIRQYFRTPVAVDDK---ADASPVTIADREAERTIRAIIEAERPDDGIYGE 70
           ERL D +  ++  +FRT ++  DK       PVT AD  AE  +R +IE   P  G+ GE
Sbjct: 11  ERLGDVASDMLLPFFRTALSARDKNIGGSFDPVTEADHAAETAMRRLIERTFPHHGVIGE 70

Query: 71  EFGTKNLDAEWVWVIDPIDGTKSFITGRPIFGTLIALLHRGRPVLGVIDQPIVRDRWLGV 130
           EFG+   DAE+VWV+DPIDGTKSFI G PI+G+LI LLH GRP  G++ QP  ++R++G 
Sbjct: 71  EFGSVRSDAEYVWVLDPIDGTKSFIAGLPIWGSLIGLLHNGRPTYGMMVQPFTQERFIG- 129

Query: 131 EGRPTLFNG---------QPARVRECAGGLAAATLGTTSPDLFPGADQDAFRRVAGAAKV 181
           +G   ++ G         +    R C   L  ATL TTSP L+     +AFRRV    ++
Sbjct: 130 DGAGAVWRGPGHDHKIIERKLTARSCE-DLGKATLLTTSPSLYAEPQLEAFRRVEAQTRL 188

Query: 182 SVYGGDCYSYGLLAAGYYDLVVESGLKLYDFAALVPVVTGAGGLMTDWDGRPLDATSSGR 241
           S Y GDCY++ +LAAG+ D V+ESGLK YD A L+P++ GAGG++TDW G   DAT  G 
Sbjct: 189 SRYSGDCYAFAMLAAGHVDCVIESGLKTYDIAPLIPIIEGAGGVVTDWKGG--DATGGGD 246

Query: 242 VVAAGDARTHRETLAALAG 260
           VVA GD + H + L  L G
Sbjct: 247 VVACGDRKLHEQALKLLRG 265


Lambda     K      H
   0.320    0.139    0.424 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 249
Number of extensions: 15
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 260
Length of database: 265
Length adjustment: 25
Effective length of query: 235
Effective length of database: 240
Effective search space:    56400
Effective search space used:    56400
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 47 (22.7 bits)

Align candidate WP_026607886.1 METAC_RS0116440 (histidinol-phosphatase)
to HMM TIGR02067 (hisN: histidinol-phosphatase (EC 3.1.3.15))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR02067.hmm
# target sequence database:        /tmp/gapView.25228.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR02067  [M=252]
Accession:   TIGR02067
Description: his_9_HisN: histidinol-phosphatase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
    2.7e-97  311.2   0.0      3e-97  311.0   0.0    1.0  1  lcl|NCBI__GCF_000427445.1:WP_026607886.1  METAC_RS0116440 histidinol-phosp


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000427445.1:WP_026607886.1  METAC_RS0116440 histidinol-phosphatase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  311.0   0.0     3e-97     3e-97       2     251 ..       7     263 ..       6     264 .. 0.97

  Alignments for each domain:
  == domain 1  score: 311.0 bits;  conditional E-value: 3e-97
                                 TIGR02067   2 lalalelaeaageailkyfrasdlkvdkksdk..tpVteADraaEeaireliaakfPddgilGEEfgee 68 
                                               +a++++l ++a +++l++fr+  ++ dk+     +pVteAD+aaE a+r+li+++fP++g++GEEfg+ 
  lcl|NCBI__GCF_000427445.1:WP_026607886.1   7 AAFVERLGDVASDMLLPFFRTALSARDKNIGGsfDPVTEADHAAETAMRRLIERTFPHHGVIGEEFGSV 75 
                                               6899******************999999999899*********************************** PP

                                 TIGR02067  69 eedaeyvWvlDPiDGTksFirGvPvwgtLiaLlekgkpvlGvisqPalgerfvaakgegallngge... 134
                                               ++daeyvWvlDPiDGTksFi+G+P+wg Li+Ll++g+p+ G++ qP+++erf+++  +++++ +g+   
  lcl|NCBI__GCF_000427445.1:WP_026607886.1  76 RSDAEYVWVLDPIDGTKSFIAGLPIWGSLIGLLHNGRPTYGMMVQPFTQERFIGDGAGAVWRGPGHdhk 144
                                               **********************************************************99999855689 PP

                                 TIGR02067 135 ...relrvsevaklsdAvlvttspkaledeeereafeklrrkarltryggdcyayalvAsGrvdlvvea 200
                                                  r+l+++++ +l +A+l+ttsp +l +e + eaf+++++++rl+ry gdcya+a++A+G+vd v+e+
  lcl|NCBI__GCF_000427445.1:WP_026607886.1 145 iieRKLTARSCEDLGKATLLTTSP-SLYAEPQLEAFRRVEAQTRLSRYSGDCYAFAMLAAGHVDCVIES 212
                                               999*********************.8999**************************************** PP

                                 TIGR02067 201 elspyDiaalipiieeAggvitdwkGkeaeeggeavaaanaalhdevlell 251
                                               +l++yDia+lipiie+Aggv+tdwkG +a+ gg++va+++++lh+++l+ll
  lcl|NCBI__GCF_000427445.1:WP_026607886.1 213 GLKTYDIAPLIPIIEGAGGVVTDWKGGDATGGGDVVACGDRKLHEQALKLL 263
                                               ************************************************998 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (252 nodes)
Target sequences:                          1  (265 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.00
# Mc/sec: 7.79
//
[ok]

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis