Alignments for a candidate for trpE in Desulfovibrio zosterae DSM 11974

GapMind for Amino acid biosynthesis

Alignments for a candidate for trpE in Desulfovibrio zosterae DSM 11974

Align Anthranilate synthase; EC 4.1.3.27 (characterized, see rationale)
to candidate WP_027721082.1 H589_RS0105320 anthranilate synthase component I family protein

Query= uniprot:B8DM47_DESVM
         (520 letters)



>NCBI__GCF_000425265.1:WP_027721082.1
          Length = 471

 Score =  540 bits (1392), Expect = e-158
 Identities = 279/456 (61%), Positives = 328/456 (71%), Gaps = 1/456 (0%)

Query: 53  EIVLRQTGRWLEADVDTPISVFLGMVGSGQGILLESAEVDGRWGRFSVIAFNFLLRLGCR 112
           +I L Q G+WL AD  TPIS++LG+VG   GILLESAEVDGR GR+S+IA++F L+L   
Sbjct: 2   KIELTQYGKWLPADTQTPISLYLGLVGDAPGILLESAEVDGRLGRYSLIAWDFRLKLSPV 61

Query: 113 DGKLEVAVRDPRLAPLRRFDGMGFIEGTRAVMRALRIEPDAAFADQPPITRALYGYFGYG 172
            GKL V   D RLA L  + GM F++G RAVM+AL I P     + P +TR LYG  GYG
Sbjct: 62  SGKLSVECADSRLAGLATYSGMDFLDGMRAVMKALHINPQPEVGELPSLTRGLYGTMGYG 121

Query: 173 VSGLFEPKLAKVLPTSSAEACLALPGTVVLFDHLYNRLCQLSLTDLPGGRVDRSQVDRTP 232
           ++G+ EPKL   LP   AE  LALPG VVLFDHL +  C LSL      +          
Sbjct: 122 IAGMLEPKLKDKLPAEDAEVRLALPGRVVLFDHLKHSCCFLSLDKDATPQFTPPVFGAEC 181

Query: 233 EPPEVGPVVNVPEQAVYTRAVARVKDMIRQGEAIQVVLSTRFQASFSGDPFTLYRRLRRI 292
           EP +VG  V VP +  Y   V +VKD+I  GE IQVVLSTRF A FSGD F LYRRLR+ 
Sbjct: 182 EPTKVGEPVAVPGKEKYMSGVRKVKDLIADGECIQVVLSTRFSAPFSGDSFDLYRRLRQA 241

Query: 293 NPSPYMFFMRLPGVS-LLGSSPEVMVRCRADKLQVSPIAGTRPRGTDDAHDAALARELLE 351
           NPSP+MF+M+      LLGSSPE+M RC   +L+V PIAGTRPRG D A D   A EL+ 
Sbjct: 242 NPSPFMFYMKFSREEILLGSSPEMMARCEKGRLEVRPIAGTRPRGKDAAGDRKYAEELMA 301

Query: 352 DPKERAEHVMLVDLGRNDLGRIAAPGTVQVERFMDVEKFSHVMHLTSRVTAQIEPGRDAL 411
           DPKERAEHVMLVDLGRNDLGRIA PG+V VE+FM +E FSHVMH+TS V A +    DAL
Sbjct: 302 DPKERAEHVMLVDLGRNDLGRIAKPGSVSVEKFMQIEYFSHVMHITSYVEADLRDDHDAL 361

Query: 412 DVLAATFPAGTVSGAPKVRAMEIISEAEGLARGPYAGAIGWLGLDRDSVNLDTGITIRSL 471
           DVL ATFPAGT+SGAPK+RAMEIISE E + RGPY G IG++GLD+DSVNLDTGITIRS+
Sbjct: 362 DVLQATFPAGTLSGAPKIRAMEIISEIEDVPRGPYGGCIGFIGLDKDSVNLDTGITIRSM 421

Query: 472 WVRDGQVHWQAGAGIVFDSVPEMEWKECNNKAAVIR 507
           W+RDG+ HWQAGAGIV+DS  EMEW ECNNKA V+R
Sbjct: 422 WIRDGKCHWQAGAGIVYDSDLEMEWLECNNKARVLR 457


Lambda     K      H
   0.320    0.137    0.413 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 717
Number of extensions: 18
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 520
Length of database: 471
Length adjustment: 34
Effective length of query: 486
Effective length of database: 437
Effective search space:   212382
Effective search space used:   212382
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis