Align glycine hydroxymethyltransferase (EC 2.1.2.1) (characterized)
to candidate WP_028310448.1 H566_RS0104140 serine hydroxymethyltransferase
Query= BRENDA::B4ECY9 (415 letters) >NCBI__GCF_000482785.1:WP_028310448.1 Length = 417 Score = 673 bits (1737), Expect = 0.0 Identities = 337/414 (81%), Positives = 365/414 (88%) Query: 1 MFDRAQSTIANVDPEIFAAIEQENRRQEDHIELIASENYTSPAVMAAQGSQLTNKYAEGY 60 MF+RA TIA+VDPE+++AI+ EN RQ+DHIELIASENYTSPAVM AQGSQLTNKYAEGY Sbjct: 1 MFNRATHTIASVDPELWSAIQAENGRQQDHIELIASENYTSPAVMEAQGSQLTNKYAEGY 60 Query: 61 PGKRYYGGCEYVDVVEQLAIDRVKQLFGAEAANVQPNSGSQANQGVFFAMLKPGDTIMGM 120 PGKRYYGGCEYVDVVEQLAIDR+K+LFGAEAANVQPNSGSQANQ VF A LKPGDTIMGM Sbjct: 61 PGKRYYGGCEYVDVVEQLAIDRLKKLFGAEAANVQPNSGSQANQAVFMAFLKPGDTIMGM 120 Query: 121 SLAHGGHLTHGSPVNMSGKWFNVVSYGLNENEDIDYDAAEKLANEHKPKLIVAGASAFAL 180 SLA GGHLTHG +N SGKWFNVVSYGLNE EDIDYD E LA EHKPKLI+AGASAFAL Sbjct: 121 SLAEGGHLTHGMALNQSGKWFNVVSYGLNEAEDIDYDRMEALAREHKPKLIIAGASAFAL 180 Query: 181 KIDFERLAKIAKSVGAYLMVDMAHYAGLIAAGVYPNPVPHADFVTTTTHKSLRGPRGGVI 240 +IDFER KIAK VGA MVDMAHYAGLIAAGVYPNPVP AD VT+TTHKSLRGPRGG+I Sbjct: 181 RIDFERFGKIAKEVGAIFMVDMAHYAGLIAAGVYPNPVPFADVVTSTTHKSLRGPRGGII 240 Query: 241 LMKAEYEKPINSAIFPGIQGGPLMHVIAAKAVAFKEALSPEFKEYQQKVVENARVLAETL 300 LMKAE+EK INSAIFPGIQGGPLMHVIA KAVAF+EAL PEFK YQ++V NA+V+AETL Sbjct: 241 LMKAEHEKAINSAIFPGIQGGPLMHVIAGKAVAFQEALQPEFKAYQEQVARNAKVMAETL 300 Query: 301 VKRGLRIVSGRTESHVMLVDLRAKHITGKAAEAALGAAHITVNKNAIPNDPEKPFVTSGI 360 KRGLRIVSGRTESHVMLVDLRA ITGK AE LG AH+TVNKN IPNDPEKPFVTSGI Sbjct: 301 TKRGLRIVSGRTESHVMLVDLRALKITGKEAEKLLGEAHLTVNKNGIPNDPEKPFVTSGI 360 Query: 361 RLGSPAMTTRGFGPAEAEQVGNLIADVLENPEDAATIERVRAQVAELTKRFPVY 414 R+G+PA+TTRGF AEAE+ NLIADVL P DAA +ERVRA+V +LT FPVY Sbjct: 361 RIGTPALTTRGFKEAEAEKTANLIADVLAAPGDAAVLERVRAEVKKLTDAFPVY 414 Lambda K H 0.316 0.132 0.376 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 623 Number of extensions: 20 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 415 Length of database: 417 Length adjustment: 31 Effective length of query: 384 Effective length of database: 386 Effective search space: 148224 Effective search space used: 148224 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 50 (23.9 bits)
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory